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Stephanotis total alkaloid extract as well as preparation method and application thereof

A technology of total alkaloids and Baiyaozi, which is applied in the field of medicine, can solve the problems of unstable, unclear, and large dosage of medicinal components.

Active Publication Date: 2013-01-16
ANHUI JIUFANG DRUG RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the decoction of the decoction is cumbersome, backward, the dosage is large, and it cannot play a first-aid role, and the active ingredients are unstable, unclear, and there are too many invalid impurities, which is not conducive to the promotion and clinical application of Baiyaozi in the treatment of leukopenia. Therefore, it is imperative to develop high-standard, high-efficiency, long-acting, quick-acting, and safe drugs for the treatment of leukopenia.

Method used

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  • Stephanotis total alkaloid extract as well as preparation method and application thereof
  • Stephanotis total alkaloid extract as well as preparation method and application thereof
  • Stephanotis total alkaloid extract as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Extraction method of total alkaloids of Baiyaozi:

[0031] Take the prescribed amount of Baiyaozi and add 6 times the amount of ethanol for countercurrent dynamic heating extraction, each time for 3 hours, collect the extract, filter, and concentrate the filtrate under reduced pressure to (temperature 70°C, vacuum number -0.08Npa) per 1ml is equivalent to the original Dissolve 5g of medicinal material in hot water, add hydrochloric acid to acidify, stir, stand still, filter to obtain the filtrate, refrigerate and stand for 48 hours, filter, add alkali to alkalinize, stir, stand still, filter to obtain the filtrate, and the obtained medicinal solution Molecular interception filtration is carried out through a ceramic membrane filter. The pore size of the ceramic membrane filter is 0.1 μm to 0.3 μm, and the resulting filtrate is the fine filtrate; the fine filtrate is passed through the treated ionic resin column, washed with water to remove impurities, and then 85% Elute...

Embodiment 2

[0034] Extraction method of total alkaloids of Baiyaozi:

[0035] Take the prescribed amount of Baiyaozi and add 8 times the amount of ethanol for countercurrent dynamic heating extraction, each time for 2 hours, collect the extract, filter, and concentrate the filtrate under reduced pressure to (temperature 70°C, vacuum number -0.08Npa). Each 1ml is equivalent to the original 4g of medicinal materials, dissolved in hot water, acidified with hydrochloric acid, stirred, allowed to stand, filtered to obtain the filtrate, refrigerated and left to stand for 48 hours, filtered, added alkali to alkalize, stirred, left to stand, filtered to obtain the filtrate, and the obtained medicinal solution Molecular interception filtration is carried out through a ceramic membrane filter. The pore size of the ceramic membrane filter is 0.1 μm to 0.2 μm, and the resulting filtrate is the fine filtrate; the fine filtrate is passed through the treated ionic resin column, washed with water to remov...

Embodiment 3

[0037] Extraction method of total alkaloids of Baiyaozi:

[0038] Take the prescription amount of Baiyaozi and add 5 times the amount of ethanol for countercurrent dynamic heating extraction, each time for 4 hours, collect the extract, filter, and concentrate the filtrate under reduced pressure to (temperature 70°C, vacuum number -0.08Npa) per 1ml is equivalent to the original Dissolve 5g of medicinal material in hot water, add sulfuric acid to acidify, stir, stand still, filter to obtain the filtrate, refrigerate and stand for 48 hours, filter, add alkali to alkalinize, stir, stand still, filter to obtain the filtrate, and the obtained medicinal solution Molecular interception filtration is carried out through a ceramic membrane filter. The pore size of the ceramic membrane filter is 0.2 μm to 0.3 μm, and the resulting filtrate is the fine filtrate; the fine filtrate is passed through the treated ionic resin column, washed with water to remove impurities, and then washed with ...

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Abstract

The invention discloses a stephanotis total alkaloid extract as well as a preparation method of the stephanotis total alkaloid extract. The preparation method of the stephanotis total alkaloid extract comprises the following steps of: grinding the dried stephanotis, heating and extracting, and decompressing and condensing the filtrate; diluting with heating water, and acidifying and filtrating to obtain a filtrate; alkalifying and filtrating to obtain the filtrate, and performing molecular weight cut off filtration on the obtained filtrate through a ceramic membrane filter; extracting with n-butyl alcohol, decompressing and condensing; spray drying the concentrated solution to obtain a dried powder extract; adding ethanol for ultrasonic extraction, mixing the extracting solution, absorbing the extracting solution by a sephadex column, eluting with 90% to 95% of ethanol, segmentally collecting, identifying through a high performance thin layer chromatography, mixing the parts containing alkaloids, freezing at a low temperature, and separating out an alkaloid crystal crude product; and adding ethanol for dissolving, standing and freezing to obtain a stephanotis total alkaloid pure product. A stephanotis total alkaloid medicinal preparation provided by the invention has the effect of adjuvant therapy on leucopenia, in particular to leucopenia of the tumor patients caused by radiotherapy and radiotherapy, and the leucopenia caused by other medicines.

Description

technical field [0001] The invention mainly relates to the technical field of medicine, in particular to a total alkaloid extract of Baiyaozi and its preparation method and application. Background technique [0002] Baiyaozi is the root tuber of Stephania chinensis, a plant of Fangjiaceae. It grows in fertile and moist grass, in the shade of hillside roadsides or in shrubs, and also on limestone hills; harvested in summer and autumn, sliced ​​and dried in the sun. Rich in resources, it is distributed in central and southern my country. It has the effect of clearing heat and detoxifying; expelling wind and relieving pain; cooling blood and stopping bleeding. It is used in ancient Chinese medicine for treatment throat Swelling pain, pyretic carbuncle, rheumatic arthralgia, abdominal pain Diarrhea , vomiting blood and epistaxis, traumatic bleeding, etc. Bitter and pungent, cool. [0003] Modern Chinese medicine often uses this product to treat various infections, and has ...

Claims

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Application Information

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IPC IPC(8): A61K31/4748A61K36/59A61P7/00
Inventor 邵旭何万龙李晓亮牛海军
Owner ANHUI JIUFANG DRUG RES INST CO LTD
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