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Method for establishing hypothyroidism Wistar rat orbit model by adopting radioactive internal radiation

A technology for thyroid function and radioactivity, applied in X-ray/γ-ray/particle irradiation therapy, veterinary equipment, medical science, etc., to achieve easy-to-repeat, low-cost, and simple-to-make effects

Active Publication Date: 2015-06-03
SHANGHAI PUTUO DISTRICT LIQUN HOSPITAL
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, the orbital model of hypothyroidism induced by direct internal irradiation of hyperthyroid animals has not been found in the literature at home and abroad

Method used

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  • Method for establishing hypothyroidism Wistar rat orbit model by adopting radioactive internal radiation
  • Method for establishing hypothyroidism Wistar rat orbit model by adopting radioactive internal radiation
  • Method for establishing hypothyroidism Wistar rat orbit model by adopting radioactive internal radiation

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Embodiment

[0035] The purpose of the present invention is to simulate hyperthyroidism (hyperthyroidism) in the radioactive iodine-131 ( 131 I) The method of internal irradiation, to establish a simple and effective orbital animal model of hypothyroidism (hypothyroidism), whose pathological manifestations are similar to thyroid-associated ophthalmopathy (TAO), for observation and discussion of the pathogenesis of TAO. The present invention passes 131 I injected Wistar rats intraperitoneally, and then stimulated Wistar rats with high-purity human thyroid-stimulating hormone (hTSH). Wistar rats developed hypothyroidism. Observe the animal thyroid and orbital tissues to judge whether the model is successful.

[0036] 1. Materials and methods

[0037] 1 material

[0038] 1.1 Animals 20 Wister rats (half male and half male, average weight 220g) were provided and raised by the Animal Experiment Center of the Institute of Radiological Medicine, Fudan University.

[0039] 1.2 Main reagents 13...

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Abstract

The invention relates to a method for establishing a hypothyroidism Wistar rat orbit model by adopting radioactive internal radiation. The method comprises the following steps of: (a), performing radioactive 131I internal radiation on a Wistar rat; (b), performing intra-peritoneal injection on the Wistar rat by using human thyrotrophic hormone; and (c), observing distribution of orbit somatostatin receptors. The method has the advantages that the animal model is easy to manufacture, and the method is low in cost and easy to repeat; the success rate is high, 85 percent of rats have hypothyroidism, and the thyroid tissues have the universal characteristic of the hypothyroidism; complete characteristics of thyroid related eye diseases are reflected; the method discovers that the orbit somatostatin receptors are distributed abnormally; and the death rate of animals is low in experiments.

Description

technical field [0001] The invention relates to a method for constructing an animal model, specifically, a method for establishing an orbital model of Wistar rats with hypothyroidism by using radioactive internal irradiation for observing the distribution and expression of orbital receptors. Background technique [0002] clinically 131 If hypothyroidism occurs during the treatment of GD hyperthyroidism, there is a high risk of TAO deterioration, and some patients may induce the occurrence of Graves ophthalmopathy (GO). There is no suitable animal model to observe the pathological changes of such orbital tissues. [0003] long been looking for 131 The cause of hypothyroidism-induced GO after I treatment of GD, and the observation of orbital histological changes in animal models to understand its pathogenesis. Currently, animal models of hyperthyroidism or hypothyroidism and related eye diseases have been produced by various methods. [0004] 1. First of all, the choice of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61D7/00A61N5/10
Inventor 李方都褚俏梅刘兵蔡德生药小萍
Owner SHANGHAI PUTUO DISTRICT LIQUN HOSPITAL
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