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Bdellovibrio disinfectant as well as preparation method and application thereof

A leech vibrio and disinfectant technology, applied in the field of microbial preparations, can solve the problems of large damage to microbial cells, high cost of leech vibrio granules, unsuitable for large-scale industrial production, etc., and achieves long storage time, vitality and mechanical strength. good effect

Inactive Publication Date: 2013-01-16
QINGDAO SANDING SANITARY PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the formation conditions of the polymer network are relatively severe, the damage to the microbial cells is relatively large, and the diversity and controllability of the formation are not good.
Zhang Xiaoneng et al. studied the optimization of the preparation conditions of Bdellovibrio granules by sodium alginate embedding method in "Optimization of Preparation Conditions for Bdellovibrio Phage Granules". Vibrio granules are expensive and not suitable for large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation of Bdellovibrio bacteria liquid:

[0023] Inoculate the Bdellovibrio strain in 100mL 1 / 10NB medium, and add 100L at the same time to a concentration of 1.0×10 6 CFU / mL Escherichia coli suspension was cultured on a shaker at a temperature of 35°C at 100r / min for 48h. The total number of colonies was determined to be 1.0×109 CFU / mL.

[0024] (2) Preparation of Bdellovibrio embedded particles:

[0025] Take 100g of agar powder, prepare a 1% mass fraction of agar colloid solution with sterile distilled water, take the Budovibrio bacteria solution prepared in step (1) and mix it with a volume ratio of 20:100, and put it in a mixer. The volume ratio of peanut oil, peanut oil and agar colloid-Budovibrio mixed solution is 1:100, and glutaraldehyde crosslinking agent is added to make the volume fraction of glutaraldehyde in the solution 0.1%.

[0026] Stir the stirrer at a constant temperature of 30°C for 30 minutes, quickly suck into a sterile syringe, and drop into 1% ...

Embodiment 2

[0028] (1) Preparation of Bdellovibrio bacteria liquid:

[0029] Inoculate the Bdellovibrio strains in 100mL 1 / 10NB medium, and add 100L of E. coli suspension with a concentration of 1.0×109CFU / mL at the same time, shake culture at 35℃ for 48h at 800r / min, and detect the total number of colonies The concentration of Bdellovibrio bacteria liquid is 1.0×10 13 CFU / mL.

[0030] (2) Preparation of Bdellovibrio embedded particles:

[0031] Take 1000g of agar powder, prepare a 10% concentration of agar colloid solution with sterile distilled water, take the Budovibrio bacteria solution prepared in step (1) and mix, the volume ratio of the two is 50:100, and put it into the mixer. The volume ratio of peanut oil, peanut oil and agar colloid-Bridal Vibrio mixed solution is 5:100, and glutaraldehyde crosslinking agent is added to make the volume fraction of glutaraldehyde in the solution 1%.

[0032] The stirrer was stirred at a constant temperature of 30°C for 30 minutes, quickly sucked into a...

Embodiment 3

[0034] (1) Preparation of Bdellovibrio bacteria liquid:

[0035] Inoculate Bdellovibrio strains in 100mL1 / 10NB medium, and add 100L at the same time to a concentration of 1.0×10 9 CFU / mL Escherichia coli bacteria suspension, shake culture at a temperature of 35°C at 500r / min for 48h on a shaker, and the total number of colonies detected Bdellovibrio bacteria concentration was 1.0×10 13 CFU / mL.

[0036] (2) Preparation of Bdellovibrio embedded particles:

[0037] Take 500g of agar powder and prepare a 5% mass fraction of agar colloid solution with sterile distilled water. Take the Budovibrio bacteria solution obtained in step (1) and mix it with a volume ratio of 30:100 and put it in a mixer. The volume ratio of peanut oil, peanut oil and agar colloid-Budovibrio mixed liquid is 3:100, and glutaraldehyde crosslinking agent is added to make the volume fraction of glutaraldehyde in the solution 0.5%.

[0038] The stirrer was stirred at a constant temperature of 30°C for 30 minutes, quickl...

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Abstract

The invention discloses a bdellovibrio disinfectant as well as a preparation method and application thereof and particularly discloses a phage bdellovibrio granula which belongs to the field of microbial immobilization. The method comprises the steps as follows: (1), preparation of bdellovibrio liquid: bdellovibrio strain is inoculated in colon bacillus suspension for enrichment culture so as to enable the concentration of the bdellovibrio liquid to be 1.0*109 CFU / mL to 1.0*1013 CFU / mL; and (2), preparation of bdellovibrio embedded granula: stirring the mixture of bdellovibrio liquid, agar colloidal solution, glutaraldehyde and peanut oil for 30 min at a constant temperature, then dripping the mixture in calcium chloride for immobilization, and freeze drying for 24 h to obtain bdellovibrio granula. By combination of the imbedding method and the glutaraldehyde crosslinking method, the invention prepares the phage bdellovibrio granula which has better vitality and mechanical strength, is economical and safe, can be stored for a long time, and can be applied to a plurality of industries like aquaculture and livestock and poultry raising.

Description

Technical field [0001] The present invention relates to a microbial preparation, in particular to its preparation method and use, in particular to a bacteriophagic Bdellovibrio granule for disinfection in aquaculture. Background technique [0002] Bdellovibrio bacteriovorus (hereinafter referred to as Bdellovibrio bacteriovorus) is a parasitic bacteria that specializes in preying on bacteria. Studies have pointed out that Bdellovibrio bacteriovorus can purify the water quality of aquaculture water and control or reduce the occurrence of aquatic animal diseases. The effect is remarkable with the popularity. In recent years, there have been more and more studies on the application of Bdellovibrio in shellfish, shrimp, crab, fish and other aquaculture. These studies have shown that the addition of Bdellovibrio in the breeding process can cause disease. The effects of prevention and treatment, improvement of survival rate, increase in body length and purification of the breeding eco...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N25/12A01P1/00
Inventor 邵绪霞
Owner QINGDAO SANDING SANITARY PROD
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