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Disinfectant used for aquiculture and preparation method of same

A disinfectant and solvent technology, applied in the directions of disinfectants, botanical equipment and methods, and chemicals for biological control, can solve the problems of high cost and few disinfection and sterilization products in aquaculture environments, and achieve cost-effective Low effect, simple preparation method

Inactive Publication Date: 2013-01-16
QINGDAO SANDING SANITARY PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the industrial production and utilization of Bdellovibrio is mainly solid preparations, which are generally prepared by freeze-drying methods, and the cost is relatively high, and there are few disinfection and sterilization products for aquaculture environments.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The disinfectant is obtained through the following steps:

[0019] A. prepare the liquid culture medium of the host bacterium of Bdellovibrio, culture medium is made up of following composition: sodium chloride, potassium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, percentage by weight is 5%, the peptone of 5%, 5% yeast powder, 5% beef extract, the pH of the medium is 7.0, and the medium is sterilized at 121°C for 20 minutes;

[0020] B. Put the culture medium prepared in step A into a fermenter, and implant it into the host bacteria of Bdellovibrio, and cultivate it with ventilation at 37°C for 24 hours. The total number of colonies is detected to ensure that the number of host bacteria is greater than 1×10 9 CFU / mL;

[0021] C. Take 3g Bdellovibrio preparation, prepare 3% Bdellovibrio species with sterile water, inoculate the Bdellovibrio species in the fermenter equipped with step B preparation, and culture with aeration at 37°C for 48 hou...

Embodiment 2

[0027] The disinfectant is obtained through the following steps:

[0028] A. Utilize sterile water to prepare Bdellovibrio liquid culture medium, culture medium is made up of following composition: sodium chloride, potassium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, percentage by weight is 1%, the peptone of 5%, 1% yeast powder, 1% beef extract, and use phosphate buffer to make the p of the medium 8.0, and sterilize the medium at 121°C for 20 minutes;

[0029] B. Put the culture medium prepared in step A into the fermenter, and put Escherichia coli, the number of bacteria is greater than 1×10 9 CFU / mL, 35°C ventilation culture for 24 hours, the total number of colonies detected to ensure that the number of host bacteria is greater than 1×10 9 CFU / mL

[0030] C. Take 1g of Bdellovibrio preparation, prepare 1% Bdellovibrio species with sterile water, inoculate it in the fermenter prepared in step B, culture it with ventilation at 35°C for 24 hours,...

Embodiment 3

[0035] The disinfectant prepared by the present invention is applied to the breeding test of abalone, and its influence on specific breeding animals is detected.

[0036] Using the disinfectant in Example 1, and setting up a blank control, observe the growth status of abalone and the number of main microorganisms in the water body under different culture conditions.

[0037] In this experiment, the method of adding Bdellovibrio preparations combined with periodic water changes was used to study the application potential of Bdellovibrio in Abalone abalone culture. The current production ponds were used as the control group A, and different concentrations of disinfectant were added to the water body of the experimental group. agent, so that the concentration of Bdellovibrio in the culture pond was 10 2 CFU / mL, 10 4 CFU / mL and 10 6 CFU / mL, respectively for group B, group C and group D, and the rest of the culture conditions are exactly the same. At the same time, the experimen...

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PUM

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Abstract

The invention discloses a disinfectant used for aquiculture and a preparation method of the disinfectant. The disinfectant is a biological disinfectant and contains 109 to 1013 CFU / mL of bdellovibrio bacteriovorus. The preparation method comprises the following steps: A, preparing liquid culture medium for host bacteria of the bdellovibrio bacteriovorus; B, placing the culture medium prepared in step A in a fermentation tank and placing the host bacteria of the bdellovibrio bacteriovorus; and C, inoculating bdellovibrio bacteriovorus strain into the fermentation tank used for preparation in step B, carrying out aerobic culture at the temperature of 35 DEG C to 37 DEG C for 24 to 48 hours, and detecting the total number of colonies to ensure that the content of bdellovibrio bacteriovorus in the fermentation tank is 109 to 1013 CFU / mL. According to the invention, the disinfectant which is efficient and environment-friendly and does not cause residues of poisonous substances to aquiculture animals is obtained through a biological control method; and besides, the preparation method is simple, the cost is relatively low, and the invention is suitable for large scale application.

Description

technical field [0001] The invention relates to a disinfectant for aquaculture, in particular to a preparation method thereof, in particular, preparing a disinfectant from a biological agent for disinfection and sterilization of an aquaculture environment. Background technique [0002] Bdellovibrio is a type of bacteria that parasitizes other bacteria and can cause them to lyse. Although it is smaller than ordinary bacteria, it can pass through bacterial filters and has a similar effect as a phage, but it is not a virus, it is indeed a type of "energy" Bacteria that eat "bacteria." In 1962, it was first discovered in Pseudomonas spp., which was subsequently isolated from soil and sewage. According to its basic characteristics, it is named Bdellovibrio bacteriovorus. Among them, the word "Bdello" comes from the Greek word, which means "leech", "vibrio" means "vibrio", and the species name "bacteriovorus" means "bacteria-eating". They have aroused the interest of many scien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P1/00C02F1/50
Inventor 邵绪霞
Owner QINGDAO SANDING SANITARY PROD
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