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Method for detecting potassium ion by preparing biosensor based on G-quadruplex and gold nanoparticle

A technology of gold nanoparticles and biosensors, which is applied in the field of biosensors, can solve problems such as narrow detection range, and achieve fast detection speed, high sensitivity, and real-time detection effects

Inactive Publication Date: 2013-01-09
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the law for K + The detection range is very narrow, 0.1-1mM, which is far from meeting the needs of the development of existing technologies

Method used

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  • Method for detecting potassium ion by preparing biosensor based on G-quadruplex and gold nanoparticle
  • Method for detecting potassium ion by preparing biosensor based on G-quadruplex and gold nanoparticle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The first step: pretreatment of gold electrodes:

[0023] (1) Immerse the gold electrode in concentrated H at a volume ratio of 3:1 at 70°C 2 SO 4 and H 2 o 2 Composed solution (piranha) for 5 minutes, then rinse with plenty of secondary water. The H 2 o 2 The quality score is 30%.

[0024] (2) The gold electrode should be polished on the polishing cloth continuously with 1.0μm, 0.3μm and 0.05μm alumina powder respectively.

[0025] (3) Clean the gold electrode ultrasonically in ethanol and secondary water: 1 minute in ethanol and 2 minutes in secondary water.

[0026] (4) The gold electrode is placed in 0.5mol L -1 H 2 SO 4 In solution, a cyclic voltammetry scan was performed between -0.2 and +1.6 V until a typical cyclic voltammetry spectrum was obtained, and a clean gold electrode was obtained.

[0027] The second step: the preparation of p-aminothiophenol (p-ATP): use absolute ethanol as a solvent, add p-ATP to the solvent to obtain an ethanol solution co...

Embodiment 2

[0037] The first step: pretreatment of the gold electrode: the pretreatment process is the same as in Example 1.

[0038] The second step: the preparation of p-aminothiophenol (p-ATP): use absolute ethanol as a solvent, add p-ATP to the solvent to obtain an ethanol solution containing 8mM p-ATP, and prepare the ethanol solution before use , Sealed and stored to prevent the liquid from evaporating.

[0039] The third step: modify p-ATP on the gold electrode;

[0040] Soak the gold electrode treated in the first step in 8mM p-ATP ethanol solution and place it at room temperature for 24 hours. Then rinse with ethanol and deionized water, and blow dry with nitrogen.

[0041] The fourth step: modifying gold nanoparticles on the electrode modified with p-ATP: add the prepared 13nm gold nanoparticle sol dropwise to the surface of the gold electrode modified with p-ATP, and leave it at room temperature for 10 hours until the electrode surface The gold nanoparticle sol almost dries ou...

Embodiment 3

[0048] The first step: pretreatment of gold electrodes:

[0049] With embodiment 1.

[0050] The second step: the preparation of p-aminothiophenol (p-ATP): use absolute ethanol as a solvent, add p-ATP to the solvent to obtain an ethanol solution containing 12mM p-ATP, and prepare the ethanol solution before use , Sealed and stored to prevent the liquid from evaporating.

[0051] Step 3: Modify p-ATP on the gold electrode:

[0052] Soak the gold electrode treated in the first step in 12mM p-ATP ethanol solution and place it at room temperature for 24 hours. Then rinse with ethanol and deionized water, and blow dry with nitrogen.

[0053] The fourth step: modifying gold nanoparticles on the electrode modified with p-ATP: add the prepared 17nm gold nanoparticle sol dropwise to the surface of the gold electrode modified with p-ATP, and place it at room temperature for 24 hours until the electrode surface The gold nanoparticle sol almost dries out. Rinse with water and blow dr...

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Abstract

The invention discloses a method for detecting a potassium ion by preparing a biosensor based on a G-quadruplex and a gold nanoparticle, and belongs to the field of the biosensor. A detection method comprises the following steps of: modifying the gold nanoparticle on the surface of a gold electrode by taking para-aminothiophenol (p-ATP) as a medium, fixing a DNA (Deoxyribose Nucleic Acid) marked by ferrocene (Fc) and capable of forming a G-quadruplex structure onto the surface of the gold nanoparticle by a sulfydryl self-assembly action, and taking the DNA as an aptamer to prepare an electrochemistry biosensor to detect the potassium ion. The method for detecting the potassium ion by preparing the biosensor based on the G-quadruplex and the gold nanoparticle has the advantages that the detection is simple and quick, the sensitivity is high, the real-time detection can be carried out on the potassium ion, the minimum detection concentration to the potassium ion reaches 0.1 mM, and the linear range of the detection is large. In a concentration range of 0.1-1 mM, a square wave volt-ampere peak current is in a well linear relation with the concentration of the potassium ion; and in a concentration range of 1-30 mM, the square wave volt-ampere peak current is in a well linear relation with the logarithm of the concentration of the potassium ion.

Description

technical field [0001] The invention belongs to the field of biosensors, and in particular relates to a method for preparing biosensors based on G-quadruplexes and gold nanoparticles to detect potassium ions. Background technique [0002] Potassium is an indispensable constant element in the human body. It plays an important role in regulating body fluid osmotic pressure and acid-base balance, maintaining cell metabolism, and ensuring normal neuromuscular functions. Its content is an important indicator of human physiological activities, and its level in blood and urine is particularly important for clinical diagnosis, so the determination of potassium ion content is of great significance. Reference [1]Abd-Elgawad Radi and Ciara K.O'Sullivan.Aptamer conformational switch as sensitive electrochemical biosensor for potassium ion recognition.Chem.Commun., 2006,3432-3434 records, Abd-Elgawad Radi et al. Ferrocene (Fc)-labeled nucleotide chains that can form a G-quadruplex struc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327
Inventor 李丽东黄晓青衣晓辉律宇丰
Owner BEIHANG UNIV
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