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Lactobacillus jensenii for preventing and curing bacterial vaginosis

A technology based on Lactobacillus jansnii and bacteria, which is applied in the field of Lactobacillus jansnii for the prevention and treatment of bacterial vaginosis, and can solve problems such as poor effect and toxic side effects

Active Publication Date: 2013-10-23
北京益生苑生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the ineffectiveness of antibiotics in the treatment of bacterial vaginosis and the side effects of liver and kidney damage caused by antibiotics, efforts have been made to explore microecological therapies based on probiotic Lactobacillus preparations at home and abroad.

Method used

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  • Lactobacillus jensenii for preventing and curing bacterial vaginosis
  • Lactobacillus jensenii for preventing and curing bacterial vaginosis
  • Lactobacillus jensenii for preventing and curing bacterial vaginosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, isolation and identification of Lactobacillus jansnii strain

[0044] 1. Isolation of Lactobacillus jansii strains

[0045] MRS medium: peptone 10.0g; beef extract 10.0g; yeast extract 5.0g; diammonium hydrogen citrate 2.0g; glucose 20.0g; Tween 80, 1.0mL; sodium acetate (CH 3 COONa·3H 2 O) 5.0g; Dipotassium hydrogen phosphate (K 2 HPO4·3H 2 O) 2.0g; magnesium sulfate (MgSO 4 ·7H 2 O) 0.58g; manganese sulfate (MnSO 4 ·H 2 O) 0.25g; distilled water 1000mL; adjust to pH 6.2~6.6.

[0046] More than 200 samples of vaginal secretions from healthy women without symptoms and signs of vaginitis were collected. The samples were numbered sequentially, inoculated in MRS liquid medium, and incubated anaerobically at 37°C for 24 hours. For each sample, 5 single colonies were randomly selected and cultured in 2 mL MRS liquid medium for 24 hours, and the colonies were numbered sequentially. Bacteria are amplified for bacterial conservation and DNA preparation. ...

Embodiment 2

[0058] Example 2. Functional Analysis of Lactobacillus jensenii IMJ-1CGMCC No.6558

[0059] 1. Lactobacillus jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 produces H 2 o 2 detection of H 2 o 2 Preparation of standard curve: firstly, 30% (v / v) H 2 o 2 (equivalent to 9.128mol / L) stock solution diluted to 1mol / L, and then 1mol / L H with 100mmol / L PIPES 2 o 2 Dilute to 0μmol / L, 20μmol / L, 40μmol / L, 60μmol / L, 80μmol / L, 100μmol / L working solution; then take 100μL of the above H 2 o 2 Mix the working solution with 100 μL of TMB (tetramethylbenzidine) at a concentration of 20 mmol / L and 2 μL of horseradish peroxidase (1 mg / mL), incubate at 16°C for 10 min, and measure the OD 630 value. end up with H 2 o 2 Concentration standard curve: y=0.0033x-0.0496, R 2 =0.992.

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Abstract

The invention discloses lactobacillus jensenii and application thereof. The lactobacillus jensenii is particularly lactobacillus jensenii IMJ-1, and the file No. of the lactobacillus jensenii IMJ-1 in the General Microbiological Culture Collection Center of China Microbial Culture Collection Management Committee is CGMCC No. 6558. The lactobacillus jensenii IMJ-1 CGMCC No.6558 has high H2O2 generation capability, can effectively suppress breeding of external pathogenic bacteria and other conditional pathogenic bacteria, and has a high binding function to epithelial cells of vagina, and accordingly, breeding of bacteria strains on the vaginal epithelium is guranteed. Besides, the lactobacillus jensenii IMJ-1 CGMCC No. 6558 has a certain application prospect in preventing and curing bacterial vaginosis.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to Lactobacillus jansnii and application thereof, in particular to a strain of Lactobacillus jansii isolated from a human body and used for preventing and treating bacterial vaginosis. Background technique [0002] Microorganisms inhabit almost every part of the human body, such as the skin, oral cavity, intestinal tract, reproductive tract, etc. There are a large number of bacteria, including many different types of bacteria, which can be roughly divided into "beneficial bacteria" and "conditional treatment". There are two types of germs, and the number and proportion of these bacteria in a healthy human body maintain a stable balance. A variety of bacteria can exist in the vagina of women, among which Lactobacillus is the most common and important beneficial bacteria to maintain a healthy vaginal micro-ecological environment, and Lactobacillus accounts for more than 90% of the total vaginal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P3/00A61K35/74A61P31/04C12R1/225A61K35/747
Inventor 朱宝利张瑞芬李晶王江
Owner 北京益生苑生物科技有限公司
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