Method for preparing thamnolic acid

A technology of sam alkyd and snow tea, which is applied in the field of preparing sam alkyd, can solve the problems that there is no industrialized preparation method of sam alkyd, and achieve the effects of controllable product quality, low cost, and increased content

Inactive Publication Date: 2013-01-02
NANJING ZELANG AGRI DEV
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  • Abstract
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  • Claims
  • Application Information

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Embodiment 1

[0016] Dry the snow tea in the shade, crush it to 60 mesh, extract it with 0.5% ascorbic acid aqueous solution, the ratio of liquid to material is 10:1, reflux extraction at 50°C for 2 times, each time for 4 hours, filter and combine the extracts, and adjust with dilute lye pH to 9.5, adsorbed through an anion exchange resin column at a flow rate of 1.5BV / h, first eluted with 2BV of deionized water, and then 4BV of 75% ethanol and hydrochloric acid (4mol / ml hydrochloric acid: 75% ethanol volume ratio is 1 :8) The mixed solution is eluted, and the ethanol is recovered to obtain the alkyd extract of Samu. Add water to disperse the extract of Sam’s alkyd, pass it through a clean macroporous adsorption resin column, wash with water to remove impurities, and then elute with 80% ethanol, collect the ethanol eluate, concentrate under reduced pressure, add 2.9 times the content of Sam’s alkyd Chromatographic silica gel, mixed evenly, dried under reduced pressure, placed on the top of ...

Embodiment 2

[0018] Dry the snow tea in the shade, crush it to 100 mesh, extract it with 0.4% sodium metabisulfite aqueous solution, the ratio of liquid to material is 12:1, reflux extraction at 50°C for 3 times, each time for 2 hours, filter and combine the extracts, and use dilute lye Adjust the pH to 10.2, and adsorb through an anion exchange resin column at a flow rate of 1.5BV / h, first elute with 1BV of deionized water, and then use 6BV of 90% ethanol and hydrochloric acid (3mol / ml 1:5) mixed solution elution, recovery of ethanol to get Sam's alkyd extract. Disperse the alkyd extract with water, pass through a clean macroporous adsorption resin column, wash with water to remove impurities, and then elute with 65% ethanol, collect the ethanol eluate, concentrate under reduced pressure, add 2.3 times the content of alkyd Chromatographic silica gel, mixed evenly, dried under reduced pressure, placed on the top of a silica gel wet column, eluted with n-hexane-acetone 7:3, collected Sam’s ...

Embodiment 3

[0020] Dried snow tea in the shade, crushed to 40 mesh, extracted with 0.2% phytic acid aqueous solution, the ratio of liquid to solid was 15:1, refluxed and extracted twice at 50°C for 6 hours each time, filtered and combined the extracts, and used dilute The lye adjusts the pH to 11.0, and adsorbs it through an anion exchange resin column at a flow rate of 1.5BV / h. The ratio is 1:6) mixed solution is eluted, and ethanol is recovered to obtain the alkyd extract of Samu. Disperse the alkyd extract with water, pass it through a clean macroporous adsorption resin column, wash with water to remove impurities, and then elute with 70% ethanol, collect the ethanol eluate, concentrate under reduced pressure, add 2.5 times the content of alkyd Chromatographic silica gel, mixed evenly, dried under reduced pressure, placed on the top of a silica gel wet column, eluted with n-hexane-acetone 7:3, collected Sam’s alkyd fraction, concentrated and dried to obtain Sam’s alkyd, wherein the con...

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Abstract

The invention discloses a method for preparing thamnolic acid, which belongs to the field of a plant extraction technology. The preparation method comprises the following steps: drying vermiculate thamnolia in the shade, crushing into powder of 40-100 meshes, then using antioxidant water as a solvent for extraction, adsorbing extracted liquid with ion exchange resin, then eluting with an eluting agent, collecting eluent to recycle the reagent, adding water for dispersion, performing chromatography with a clean macroporous resin column, washing with water and then eluting with 40-80% ethanol, collecting ethanol eluent, depressurizing and concentrating, and purifying by normal phase chromatography to obtain the thamnolic acid. The product prepared by the method has the advantages that the product quality is stable and controllable, the extraction process is simple, the cost is low and the extraction efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for preparing saam alkyd. Background technique [0002] Thamnolic acid, also known as genus acid, ground tea acid, and hard-haired usnic acid. Molecular formula is C 16 h 19 o 11 , with a molecular weight of 420.33, is a kind of phenolic acid compound, which is widely distributed in plants, and mainly exists in plants such as Litmusiaceae, Tea Stainsaceae, Ditheaceae, Chicken Skinaceae and Usneaceae. Pharmacological studies have shown that sam alkyd has antibacterial activity and can keep the growth of Staphylococcus aureus, and the highest dilution is less than 1:5000. [0003] snow tea Thamnolia vermicularis (Sw.) Ach. is a plant of the tea family, which was first recorded in "Compendium of Materia Medica Supplements". It has the effects of clearing away heat, quenching thirst, calming the nerves and nourishing the heart, and is used to treat ...

Claims

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Application Information

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IPC IPC(8): C07C69/92C07C67/48
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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