Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Zinc oxide/acid containing compositions and methods for treating and/or preventing enzymatic irritation

A composition, zinc oxide technology, applied in the direction of medical preparations containing active ingredients, anhydride/acid/halide active ingredients, drug combinations, etc., can solve problems such as inflammation and antimicrobial barrier defects, permeability barrier dysfunction, etc.

Inactive Publication Date: 2013-01-02
JOHNSON & JOHNSON CONSUMER COPANIES
View PDF22 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Increased protease activity in AD leads to abnormal desquamation, degradation of lipid processing enzymes and antimicrobial peptides, and activation of major cytokines, thereby leading to permeability barrier dysfunction, inflammation, and defects in the antimicrobial barrier

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Zinc oxide/acid containing compositions and methods for treating and/or preventing enzymatic irritation
  • Zinc oxide/acid containing compositions and methods for treating and/or preventing enzymatic irritation
  • Zinc oxide/acid containing compositions and methods for treating and/or preventing enzymatic irritation

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0080] Effect of Cations on Trypsin Inhibition .

[0081] The purpose of the following experiments was to determine whether and to what extent the following cations inhibit trypsin activity in an in vitro assay.

[0082] Inhibition of Trypsin-Induced Cleavage of Fluorescent Casein Peptides Using EnzChek TM Protease assay kit, according to the manufacturer's instructions (revised EnzChek TM Protease Assay Kit Product Information; Molecular Probes, Eugene OR) Measurements were performed Briefly, different concentrations of test material were prepared in 1x phosphate buffered saline (PBS, pH 7.4). Prepare a trypsin working solution (Sigma, St. Louis, MO, units / mL) in the digestion buffer provided in the assay kit. Prepare a stock solution of BODIPY FL casein (trypsin substrate, mg / mL) by adding 0.2 mL to the substrate vial (supplied in the kit) and prepare the final substrate by diluting in digestion buffer (pH 7.8) Working solution (10 µg / mL). After incubating trypsin (wit...

example 2

[0087] Example 2: Effect of Salt Forms of the Same Divalent Cation on Trypsin Inhibition .

[0088] The goal of the following experiments was to determine whether and to what extent different salt forms of the same divalent cation had an effect on the inhibition of trypsin activity in an in vitro assay.

[0089] Trypsin inhibitory activity was assayed as described in Example 1 and the effect of different salt forms of the same cation on trypsin inhibitory activity was assessed. Table 2 shows the molecular weight, concentration and trypsin inhibitory activity of the test compounds.

[0090] Table 2

[0091]

[0092]

[0093] The results obtained indicate that different salt forms of similar cations have different potencies in trypsin inhibitory activity.

[0094] As shown in Table 2, Zn in the chloride salt form when calibrated with molar concentration 2+ Has the highest trypsin inhibitory activity, followed by acetate, gluconate and lactate forms. Table 2 also sh...

example 3

[0096] The purpose of the following experiments was to determine whether and to what extent the addition of divalent cations to talc inhibited trypsin activity in an in vitro assay. Inhibition of Trypsin-Induced Cleavage of Fluorescent Casein Peptides Using EnzChek TM Protease assay kit, according to the manufacturer's instructions (revised EnzChek TM Protease Assay Kit Product Information; Molecular Probes, Eugene OR) for measurement. Briefly, talc stock solutions were prepared in 1x phosphate buffered saline (PBS, pH 7.4) with (1) different concentrations of divalent cation salts (as indicated in Table 3) and with (2) different concentrations of magnesium chloride (as indicated in Table 4). Prepare a trypsin working solution (Sigma, St. Louis, MO, units / mL) in the digestion buffer provided in the assay kit. Prepare a stock solution of BODIPY FL casein (trypsin substrate, mg / mL) by adding 0.2 mL to the substrate vial (supplied in the kit) and prepare the final substrate by...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Compositions and methods for treating and / or preventing conditions such as diaper rash and atopic dermatitis are disclosed. The compositions and methods are particularly useful in the treatment and prevention of diaper rash and diaper dermatitis caused by the prolonged contact of human skin with body waste. The methods employ the topical application of a trypsin-inhibiting agent to the area in need of such treatment, or the area where prevention is desired. The trypsin-inhibiting agent is preferably zinc oxide in combination with an acid so as to release a specified divalent cation, i.e., zinc.

Description

technical field [0001] The present invention relates to the use of divalent cations for the protection of human skin. More particularly, the present invention relates to the use of divalent cations in skin care compositions which can be applied topically to protect the skin from irritants, especially proteases, more particularly trypsin. The invention can be used for the prevention and treatment of diaper rash and the relief of atopic dermatitis. Background technique [0002] The skin is a natural barrier that prevents the penetration of foreign matter. The stratum corneum is the superficial keratinized layer of the skin that provides a barrier to water evaporation and reduces the penetration of undesirable molecules from the external environment. The stratum corneum is composed of dead cells called corneocytes embedded in a lipid-rich matrix of fatty acids, ceramides, and cholesterol. This structure of keratinocytes embedded in lipids is believed to provide many of the s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/30A61K8/27A61K8/92A61P17/00A61Q19/10A61L15/44
CPCA61K45/06A61K31/19A61K31/194A61K31/496A61K9/00A61K9/0014A61K9/06A61K33/30A61K31/375A61K31/7012A61K31/4174A61P17/00A61P17/02A61P17/16A61K2300/00A61K31/185
Inventor C·B·林E·T·G·E·耿恩Y-P·胡N·库尔卡尼M·C·麦克S·摩塞斯T·H·恩古耶
Owner JOHNSON & JOHNSON CONSUMER COPANIES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com