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Method for inducing development of natural tetraploid loach male nucleus in cold shock way

A technology of tetraploid loach and androgenesis, which is applied in fish farming, application, climate change adaptation, etc., can solve problems such as death, low survival rate of diploid, destruction of cytoplasm and other organelles, and avoid operating procedures , It is conducive to large-scale production and use, and the effect of shortening the breeding time

Inactive Publication Date: 2012-12-26
DALIAN OCEAN UNIV +1
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, the most commonly used methods to inactivate egg chromosomes are ionizing radiation or ultraviolet (UV) irradiation, etc. Although the method of applying radiation to inactivate the genetic material of eggs is simple and easy, but the radiation destroys the nucleus of the egg, it also affects the The cytoplasm and other organelles also cause damage to varying degrees. After the egg is fertilized, although the haploid can pass through the embryonic development well, but in the hatching period or a few days after hatching, it will be due to "haploid syndrome". die
The main method of existing chromosome doubling is to use temperature, pressure stimulation or chemical mutagen to prevent the first cleavage, however, the survival rate of the obtained diploid is extremely low

Method used

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  • Method for inducing development of natural tetraploid loach male nucleus in cold shock way
  • Method for inducing development of natural tetraploid loach male nucleus in cold shock way

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Experimental program
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Effect test

Embodiment

[0011] Embodiment carries out as follows successively:

[0012] a. Take well-developed natural male tetraploid loach (4n=100) and female diploid loach (2n=50), use the existing technology to induce labor, the water temperature is 24±1℃, and the effect time is 10~12 Hours later, after confirming ovulation, lightly press the abdomen to collect the ovum of the female diploid loach, and use a capillary to collect the semen of the male tetraploid loach, and the gained semen is diluted 100 times with Kurokura's solution solution for later use; the preparation method of Kurokura's solution solution is present Available technology, i.e. NaCl 750 mg, CaCl 2 20 mg, NaHCO 3 20 mg, KCl 20 mg dissolved in 100 ml distilled water;

[0013] b. The diluted semen and ovum are mixed to form fertilized eggs according to the method of the prior art. The difference from the prior art is that the fertilized eggs are placed in 3°C ice water, soaked for 60min, and then placed in normal temperature...

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Abstract

The invention discloses a method for inducing the development of natural tetraploid loach male nucleus in a cold shock way. The method has the advantages that the ovum inactivation and the chromosome doubling treatment are not needed, the male nucleus development diploid loach can be fast and efficiently produced, and in addition, the hatching rate is high. The method is sequentially implemented according to the following steps that the natural male tetraploid loach and female diploid loach are taken for hastening parturition, the semen of the male tetraploid loach and the ovum of the female diploid loach are respectively taken, the obtained semen is diluted by Kurokura's solution for 100 times for use, the diluted semen is mixed with the ovum to form fertilized ovum, and the fertilized ovum is placed into ice water being 3 DEG C to be soaked for 60 minutes and is then placed into normal temperature water for hatching.

Description

technical field [0001] The invention relates to a method for androgenesis of polyploid fish, in particular to a cold shock method for rapidly and efficiently producing androgenetic diploid loach with high hatching rate without egg inactivation and chromosome doubling treatment A method for inducing androgenesis in natural tetraploid loach. Background technique [0002] Androgenesis refers to the reproductive method of using sperm to produce individuals with only paternal genetic material. Since the genetic material of androgenesis comes entirely from the father, without the participation of female pronuclei, it can be used for the establishment of pure lines, and at the same time, it can freeze the sperm of endangered species, which has special significance for the protection of species. In addition, in aquaculture, because different sexes of many important economic fishes often show different production performances, such as differences in body shape and body color, growth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 李雅娟姜志强王玉生高养春刘博
Owner DALIAN OCEAN UNIV
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