Double-tray combined type plant tissue culture container

Abstract

The invention discloses a double-tray combined type plant tissue culture transparent container which comprises an upper tray, a lower tray, a ventilation filtering device, a locking mechanism, a sealing device, a liquid inlet hole, a liquid outlet hole, a culture sieve plate and a bracket, wherein the sealing device, the liquid inlet hole, the liquid outlet hole and the culture sieve plate are closely combined with one another. The container is capable of conveniently obtaining the culture conditions such as sterility, illumination, air, relative humidity, nutrition supply and the like which are needed by plant culture; and the container is proper in volume and is convenient to disassemble and store, and can be used for not only overcoming the defects of high equipment cost, easy pollution caused by inoculation and difficult moving of a large container, but also overcoming the defects of high production using cost, narrow interior space, poor air permeability, complicated working procedures and the like of the traditional small container. Therefore, the industrialized production cost of plant tissue culture can be effectively lowered, the seedling cultivation link of the plant tissue culture is fundamentally simplified, and the economic benefit of seedling breeding is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of agricultural, forestry and animal husbandry plants, and relates to a container for in vitro culture of plant tissue materials and a method for cultivating plant tissue materials using the container. Background technique [0002] Plant tissue culture, also known as plant cloning, refers to the inoculation of plant organs and tissues in artificially prepared medium containers containing nutrients and plant growth regulators through aseptic procedures, and in vitro culture in an artificially controlled environment. , the process of making it differentiate and grow to form a complete plant. Plant tissue culture provides a new means for the rapid propagation of plants. The inoculated explants can be induced to form callus and produce shoots and roots. After several generations, asexual reproduction lines can be established. Therefore, the use of plant tissue culture technology has played a d...

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Problems solved by technology

In order to ensure the safety of inoculation, the inventor designed the inoculation port to be 40cm 2 , although it can reduce the probability of contamination of culture materials, but due to the small opening, the effective expansion of container volume and the uniformity of inoculation are limited, and the problem of material contamination cannot be fundamentally solved.

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