Method for preparing pistacia chinensis bunge bud chromosome slide

Abstract

The invention provides a method for preparing a pistacia chinensis bunge bud chromosome slide. The method comprises the following steps of carrying out low-temperature pre-treatment by a 8-hydroxyquinoline solution having a concentration of 0.002 mol / L, fixing by a Carnoy's fixer, carrying out enzymolysis by a cellulase having content of 2% in a water bath kettle at a temperature of 30 DEG C for 40 minutes, fixing by the Carnoy's fixer for 20 minutes, carrying out dissociation by hydrochloric acid having a concentration of 1 mol / L in a water bath at a temperature of 60 DEG C for 5 minutes, carrying out rinsing by distilled water, cutting tissue blocks into fragments, carrying out dyeing by a carbol fuchsin dye for 15 minutes, preparing the pistacia chinensis bunge bud chromosome slide by a squashing method, carrying out microscopic examination, and carrying out photographing by an OLYMPUSCX / MD50 microscope. The method provided by the invention has simple and convenient processes, determinates optimal material drawing time, a material drawing position and a preparation method of a pistacia chinensis bunge bud chromosome slide, and realizes uniform chromosome dispersion, a shallow background, easy observation and high accuracy of the number of chromosomes.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing chromosomes of pistachio chinensis buds. Background technique [0002] Pistacia ( Pistacia chinensis Bunge) is native to my country. It is dioecious, also known as Kaimu, Kaishu, etc., and belongs to the deciduous trees of the genus Pistacia. It is resistant to drought, salinity, barrenness, strong adaptability, and wide distribution. It is an excellent tree species for greening, timber, ornamental, medicinal and oil. The oil content of Pistacia chinensis is 35%, which is an excellent raw material for biodiesel production. It has been listed by experts as one of the most promising biodiesel raw material tree species in the next 20 years, and plays an important role in solving the current and future energy crisis. . [0003] At present, there is an upsurge in research on Pistacia chinensis in our country. In the prior art, the method of direct ac...

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Problems solved by technology

[0003] At present, there has been an upsurge in research on Pistacia chinensis in our country. In the prior art, the method of direct acid hydrolysis or acid hydrolysis followed by enzymatic hydrolysis makes the chromosomes disperse unevenly In addition, because the chromosomes of Pistacia chinensis are tiny, only 1-2 μm, and the hydrolysis time is not properly controlled in the prior art, when the hydrolysis time is insufficient, the chromatin is also colored, which will reduce the contrast; and the hydrolysis time is too long, although the cytoplasm is not Coloring, chromosome staining will be lighter, and the chromosome shape and number of Pistacia chinensis cannot be clearly observed

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