Strain used for fermenting rice bran and wheat bran extracts for producing grifolan
A technology of Grifola frondosa polysaccharide and extract, applied in the directions of microorganism-based methods, fermentation, microorganisms, etc.
Inactive Publication Date: 2014-08-20
JIANGSU UNIV
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Problems solved by technology
If a suitable strain of Grifola frondosa can be screened out, a breakthrough may be made in research and production
Method used
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Embodiment 1
[0052] Example 1 Laser mutagenesis of Grifola frondosa protoplasts
[0053] a. Preparation of osmotic pressure stabilizer
[0054] 0.6mol / L of mannitol.
[0055] b. Preparation of enzymatic solution
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Abstract
The invention belongs to the field of microorganism application technology and food biotechnology, and discloses a bacterial strain for fermenting rice bran and bran extract to produce grifola frondosa polysaccharide. The grifola frondosa strain has been preserved in the China Center for Type Culture Collection (CCTCC) in Wuhan University, Wuhan, China on April 7, 2011. The preserved strain number is CCTCC No: M2011113, and the name is Grifolasp.JSU10-2. The present invention obtains a bacterial strain that utilizes cheap raw materials to produce high-yield mycelia polysaccharides through protoplast laser mutagenesis. The bacterial strain and the original strain respectively liquid-ferment rice bran and bran compound medium, and the mycelium dry weight and polysaccharide of the mutagenic strain are respectively higher than those of the original The strains improved by 31.7% and 32.6%.
Description
technical field [0001] The invention relates to the technical field of food microbiology application, in particular to a new grifola frondosa mutagenic strain suitable for growing in a compound medium of rice bran and bran extract and high-yielding the frondosa frondosa polysaccharide. Background technique [0002] Scientific research at home and abroad and the domestic market have shown that edible fungus polysaccharides can enhance human immunity and assist in the treatment of tumors and other diseases, and have become an auxiliary product for tumor biotherapy. Domestic medicinal products made from the fruiting bodies of edible fungi include Ganoderma lucidum capsules from Wuhu, Anhui, and Grifola frondosa capsules from Qingyuan, Zhejiang. Non-pharmaceutical health products made from edible fungus extracts are also sold. Internationally, New Zealand produces "GanoPoly" polysaccharide extract series products, which are sold to the United States, Australia, China (including ...
Claims
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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/04C12R1/645
Inventor 刘伟民郭春梅张建徐立平顾慧敏任晓锋李永转张志才崔凤杰赵杰文沈国栋
Owner JIANGSU UNIV
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