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Method for polymerizing and screening anti-soybean mosaic virus genes for soybean

A technology of soybean mosaic virus and screening method, which is applied in the field of soybean germplasm, can solve problems such as poor quality, low yield per unit area, and decline in soybean yield, and achieve the effects of shortening the number of years, improving efficiency, and reducing blindness

Inactive Publication Date: 2012-12-05
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

In addition to low yields, another major problem in the region is the serious damage caused by soybean virus diseases, resulting in reduced soybean yield and poor quality

Method used

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  • Method for polymerizing and screening anti-soybean mosaic virus genes for soybean
  • Method for polymerizing and screening anti-soybean mosaic virus genes for soybean
  • Method for polymerizing and screening anti-soybean mosaic virus genes for soybean

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Embodiment Construction

[0018] According to the manual attached figure 1 The schematic diagram of the screening process for disease-resistant materials is shown for cross-breeding:

[0019] Specifically, Qihuang 1 and Kefeng 1 and Dabaima and Nannong 1138-2 were crossed by conventional methods to obtain F 1 Dai, Jiang (Qihuang No. 1 / Kefeng No. 1) F 1 And (big white hemp / Nannong 1138-2) F 1 Carry out compound crossing, planting multiple crossing F 1 Generation, F harvested after selfing 2 Generation seeds, planted F 2 Generation isolation population, extract DNA, and use the molecular markers shown in Table 1 by the screening method described below to perform assisted selection on the three disease resistance genes. Screening for F using SSR markers linked to 3 resistance genes (Table 1) 2 also contains R SC14Q , R SC8 and R SC4 Individual plants with disease-resistant genes were harvested to obtain F 3 Generation, repeat the above screening operation until a single plant containing 3 homozy...

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Abstract

The invention discloses a method for polymerizing and screening anti-soybean mosaic virus genes for soybean. The method is characterized by comprising the following steps of: identifying three resistant germplasms, i.e., Qihuang No.1, Kefeng No.1 and big white grain carrying different resistant genes respectively, and a variety with good economical character, i.e., Nannong 1138-2; hybridizing the Qihuang No.1, the Kefeng No.1 and the big white grain with the Nannong 1138-2 with the conventional method to obtain F1 generations; hybridizing two F1 in a compound way; planting a compound hybridized F1 generation; inbreeding to harvest an F2 generation seed; and screening a soybean plant polymerized by using three anti-soybean mosaic virus genes by continuously using a determined molecular marker which is tightly linked with the resistant gene from the generations F2 to F5. According to the method, three resistant genes can be selected simultaneously, the blindness of disease-resistant breeding selection is reduced, and a descendant material polymerized from a plurality of homozygous resistant genes can be selected quickly, so that the disease-resistant breeding period is shortened, and the disease-resistant breeding efficiency is increased.

Description

technical field [0001] The present invention relates to soybean mosaic virus-resistant soybean gene aggregation and screening methods, specifically, the identification and development of molecular markers closely linked to resistance genes, combined with the selection of molecular marker-assisted resistance genes, to obtain three resistance genes Genetic soybean germplasm. Background technique [0002] Soybean mosaic virus (SMV) disease is a worldwide disease in soybean production, seriously affecting soybean yield and quality. Because of its wide distribution and serious damage, it is difficult to control it with chemical agents. Breeding and planting disease-resistant varieties is the most economical, safe and effective way to control this disease. [0003] The Huang-Huai-Hai River Basin is the second soybean producing area in my country and the largest producing area of ​​summer soybeans. The perennial sown area is more than 30 million mu, and the average yield per mu is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 智海剑王大刚马莹李海朝赵琳
Owner NANJING AGRICULTURAL UNIVERSITY
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