Total flavone of Hypericum ascyron L and extraction method and application thereof

An extraction method and technology for Eclipta chinensis, which are applied in the field of medicine, can solve the problem that the research on the functional components of Eclipta chinensis is few and the like, and achieve the effects of good antioxidant capacity, large adsorption capacity and simple purification process.

Inactive Publication Date: 2014-07-16
NANCHANG UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, there is still little research on the functional components of Eclipta chinensis. Application No. 200610038949 discloses a method for extracting total flavonoids in Eclipta chinensis, including 40-95% alcohol extraction, concentration, AB-8, HPD-100 macropores Steps such as resin or polyamide column, elution, concentration and drying

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  • Total flavone of Hypericum ascyron L and extraction method and application thereof

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Embodiment 1

[0032] The raw material of the whole herb of Eclipta chinensis is taken, dried at 50-60°C until the water content is 10-15%, and crushed. Take 20g of the powder, add 800mL of 60% ethanol solution, put it into a microwave oven, and use 640W power microwave to assist extraction for 4min. After the extraction, cool to room temperature, vacuum filter and determine the yield of flavonoids to be 5.9%. The filtrate was concentrated in vacuo at 50°C until no alcohol smell. Dilute the flavone concentrate to 3.5 mg / mL with distilled water and adjust the pH to 6 with dilute hydrochloric acid. The adsorption solution of flavonoids and resin was prepared at the ratio of wet weight of resin: loading volume of 1:25. The solution was placed on a constant temperature oscillator at 35 °C for 6 h of vibration adsorption. After the adsorption was completed, the solution was filtered, the resin was rinsed with 2 times the volume of distilled water, and 200 mL of 60% ethanol was added and placed...

Embodiment 2

[0034] The raw material of the whole herb of Eclipta chinensis is taken, dried at 50-60°C until the water content is 10-15%, and crushed. Take 20g of the powder, add 1000mL of 50% ethanol solution, put it into a microwave oven, and extract it with 800W power microwave for 3.5min. After the extraction, cool to room temperature, vacuum filter and determine the yield of flavonoids to be 5.6%. The filtrate was concentrated in vacuo at 50°C until no alcohol smell. The flavonoid concentrate was diluted to 3 mg / mL with distilled water and the pH was adjusted to 5 with dilute hydrochloric acid. The adsorption solution of flavonoids and resin was prepared at the ratio of wet weight of resin: loading volume of 1:25. The solution was placed on a constant temperature oscillator at 40°C for 6 h of vibration adsorption. After the adsorption, the solution was filtered, the resin was rinsed with 2 times the volume of distilled water, and 100 mL of 70% ethanol was added and placed on a shak...

Embodiment 3

[0036] The raw material of the whole herb of Eclipta chinensis is taken, dried at 50-60°C until the water content is 10-15%, and crushed. Take 20g of the powder, add 600mL of 50% ethanol solution, put it into a microwave oven, and use 640W power microwave to assist extraction for 4.5min. After the extraction, cool to room temperature, vacuum filter and determine the yield of flavonoids to be 5.5%. The filtrate was concentrated in vacuo at 45°C until no alcohol smell. The flavone concentrate was diluted to 4 mg / mL with distilled water and the pH was adjusted to 7 with dilute sodium hydroxide. The adsorption solution of flavonoids and resin was prepared at the ratio of wet weight of resin: loading volume of 1:25. The solution was placed on a constant temperature oscillator at 30°C for 6 h of vibration adsorption. After the adsorption, the solution was filtered, the resin was rinsed with 2 times the volume of distilled water, and 200 mL of 70% ethanol was added and placed on a...

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Abstract

The invention relates to total flavonoids of Eclipta chinensis and its extraction method and application, belonging to the field of medicine. The present invention extracts the total flavonoids of Eclipta chinensis through steps such as raw material drying, pulverization, extraction, and NKA-9 type macroporous resin purification. Industrial production.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to eclipta flavonoids and its extraction method and application Background technique [0002] Red Eclipta is a plant of the genus Hypericumascyron L in the family Garcinia, also known as Huang Haitang, etc. It has the effects of calming the liver, stopping bleeding, and clearing away heat and detoxification. For the treatment of bleeding, bruises, sores, boils, swelling and pain embolism. At present, there is still little research on the functional components of Eclipta chinensis. Application No. 200610038949 discloses a method for extracting total flavonoids in Eclipta chinensis, including 40-95% alcohol extraction, concentration, AB-8, HPD-100 macropores Steps such as resin or polyamide column, elution, concentration and drying. Contents of the invention [0003] The purpose of the present invention is to provide a method for rapidly and efficiently extracting total flavonoids from Eclip...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P39/06
Inventor 张国文周佳胡明明潘军辉
Owner NANCHANG UNIV
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