Total flavone of Hypericum ascyron L and extraction method and application thereof

An extraction method, the technology of Eclipta chinensis, applied in the field of medicine, can solve the problems of little research on the functional components of Eclipta chinensis, and achieve the effects of good antioxidant capacity, good selectivity, and rapid and uniform temperature rise

Inactive Publication Date: 2012-12-05
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still little research on the functional components of Eclipta chinensis. Application No. 200610038949 discloses a method for extracting total flavonoids in Eclipta chinensis, including 40-95% alcohol extraction, concentration, AB-8, HPD-100 macropores Steps such as resin or polyamide column, elution, concentration and drying

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The raw material of the whole herb of Eclipta chinensis is taken, dried at 50-60°C until the water content is 10-15%, and crushed. Take 20 g of the powder, add 800 mL of 60% ethanol solution, and put it into a microwave oven for 4 min with microwave-assisted extraction at a power of 640W. After the extraction, cool to room temperature, vacuum filter and determine the yield of flavonoids to be 5.9%. The filtrate was concentrated in vacuo at 50°C until no alcohol smell. Dilute the flavone concentrate to 3.5 mg / mL with distilled water and adjust the pH to 6 with dilute hydrochloric acid. The adsorption solution of flavonoids and resin was prepared at the ratio of wet weight of resin: loading volume of 1:25. The solution was placed on a constant temperature shaker at 35 °C for 6 h. After the adsorption was completed, the solution was filtered, the resin was rinsed with 2 times the volume of distilled water, and 200 mL of 60% ethanol was added and placed on a shaker for 2...

Embodiment 2

[0034] The raw material of the whole herb of Eclipta chinensis is taken, dried at 50-60°C until the water content is 10-15%, and crushed. Take 20 g of the powder, add 1000 mL of 50% ethanol solution, and put it into a microwave oven with 800W power for microwave-assisted extraction for 3.5 min. After the extraction, cool to room temperature, vacuum filter and determine the yield of flavonoids to be 5.6%. The filtrate was concentrated in vacuo at 50°C until no alcohol smell. Dilute the flavonoid concentrate to 3 mg / mL with distilled water and adjust the pH to 5 with dilute hydrochloric acid. The adsorption solution of flavonoids and resin was prepared at the ratio of wet weight of resin: loading volume of 1:25. The solution was placed on a constant temperature shaker at 40 °C for 6 h. After the adsorption, the solution was filtered, the resin was rinsed with 2 times the volume of distilled water, and 100 mL of 70% ethanol was added and placed on a shaker for 2 h for desorpti...

Embodiment 3

[0036] The raw material of the whole herb of Eclipta chinensis is taken, dried at 50-60°C until the water content is 10-15%, and crushed. Take 20 g of the powder, add 600 mL of 50% ethanol solution, put it into a microwave oven, and extract it for 4.5 minutes with a power of 640W. After the extraction, cool to room temperature, vacuum filter and determine the yield of flavonoids to be 5.5%. The filtrate was concentrated in vacuo at 45°C until no alcohol smell. Dilute the flavonoid concentrate to 4 mg / mL with distilled water and adjust the pH to 7 with dilute sodium hydroxide. The adsorption solution of flavonoids and resin was prepared at the ratio of wet weight of resin: loading volume of 1:25. The solution was placed on a constant temperature shaker at 30 °C for 6 h. After the adsorption was completed, the solution was filtered, the resin was rinsed with 2 times the volume of distilled water, and 200 mL of 70% ethanol was added and placed on a shaker for 2 h for desorptio...

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PUM

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Abstract

The invention relates to total flavone of Hypericum ascyron L and an extraction method and an application of the total flavone of Hypericum ascyron L, belonging to the field of medicine. The total flavone of Hypericum ascyron L is extracted through the steps of drying, smashing and extracting raw materials and purifying with NKA-9 type macroporous resin and the like. The scheme is fast in extraction speed and high in efficiency, and the extracted total flavone is high in purity and recovery rate, thereby being suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to eclipta flavonoids and its extraction method and application Background technique [0002] Red Eclipta is a plant of the genus Hypericum (Hypericum ascyron L) of Garciniaceae, also known as Huang Haitang, etc. It has the effects of calming the liver, stopping bleeding, clearing away heat and detoxifying. For the treatment of bleeding, bruises, sores, boils, swelling and pain embolism. At present, there is still little research on the functional components of Eclipta chinensis. Application No. 200610038949 discloses a method for extracting total flavonoids in Eclipta chinensis, including 40-95% alcohol extraction, concentration, AB-8, HPD-100 macropores Steps such as resin or polyamide column, elution, concentration and drying. Contents of the invention [0003] The purpose of the present invention is to provide a method for rapidly and efficiently extracting total flavonoids from Eclipt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P39/06
Inventor 张国文周佳胡明明潘军辉
Owner NANCHANG UNIV
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