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Use of sodium nitroprusside in ganoderma lucidum submerged fermentation for producing ganoderma lucidum polysaccharide

A technology of Ganoderma lucidum polysaccharide and deep fermentation, applied in the direction of fermentation, can solve the problem of undeveloped physiological and biochemical functions, and achieve the effect of increasing yield

Inactive Publication Date: 2012-11-28
ANHUI SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the physiological and biochemical functions of animal and plant signal molecule nitric oxide (NO) in edible and medicinal fungi have not yet been developed at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment one: get Ganoderma lucidum seed liquid and insert in five Erlenmeyer flasks that fill 95mL liquid culture medium (containing glucose 2.0 grams, peptone 1.0 grams, potassium dihydrogen phosphate 0.1 grams, magnesium sulfate 0.1 grams) by 5% inoculation amount When cultivating on a constant temperature shaker at 28°C and 140rpm for 3 days, add 0, 0.1, 0.2, 1.0, 2.0 mL of sodium nitroprusside aqueous solution with a concentration of 500 mmol / L, and continue culturing for 6 days to harvest the fermentation product containing Ganoderma lucidum polysaccharide ( fermentation broth). Centrifuge the fermentation broth at 5000rpm for 10min, concentrate the supernatant in a rotary evaporator at 60°C, add 4 times the volume of absolute ethanol and let it stand in a refrigerator at 4°C for 48h, centrifuge at 5000rpm for 10min to obtain a precipitate, remove the protein by Sevag method, and store at 4°C It was dialyzed with deionized water for 24 hours, concentrated in a r...

Embodiment 2

[0016] Embodiment two: get Ganoderma lucidum seed liquid and insert in five Erlenmeyer flasks that fill 95mL liquid culture medium (containing glucose 2.0 grams, peptone 1.0 grams, potassium dihydrogen phosphate 0.1 grams, magnesium sulfate 0.1 grams) by 5% inoculum When cultivating on a constant temperature shaker at 28°C and 140rpm for 5 days, add 0, 0.1, 0.2, 1.0, 2.0 mL of sodium nitroprusside aqueous solution with a concentration of 500 mmol / L, and continue culturing for 4 days to harvest the fermentation product containing Ganoderma lucidum polysaccharide ( fermentation broth). Centrifuge the fermentation broth at 5000rpm for 10min, concentrate the supernatant in a rotary evaporator at 60°C, add 4 times the volume of absolute ethanol and let it stand in a refrigerator at 4°C for 48h, centrifuge at 5000rpm for 10min to obtain a precipitate, remove the protein by Sevag method, and store at 4°C It was dialyzed with deionized water for 24 hours, concentrated in a rotary evap...

Embodiment 3

[0017] Embodiment three: get Ganoderma lucidum seed solution and insert in five Erlenmeyer flasks that fill 95mL liquid culture medium (containing 2.0 grams of glucose, 1.0 gram of peptone, 0.1 gram of potassium dihydrogen phosphate, and 0.1 gram of magnesium sulfate) by 5% inoculation amount When culturing on a constant temperature shaker at 28°C and 140rpm for 4 days, add 0, 0.1, 0.2, 1.0, 2.0 mL of sodium nitroprusside aqueous solution with a concentration of 500 mmol / L respectively, and continue culturing for 6 days to harvest the fermentation product containing Ganoderma lucidum polysaccharide ( fermentation broth). Centrifuge the fermentation broth at 5000rpm for 10min, concentrate the supernatant in a rotary evaporator at 60°C, add 4 times the volume of absolute ethanol and let it stand in a refrigerator at 4°C for 48h, centrifuge at 5000rpm for 10min to obtain a precipitate, remove the protein by Sevag method, and store at 4°C It was dialyzed with deionized water for 2...

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Abstract

The invention discloses a use of sodium nitroprusside in ganoderma lucidum submerged fermentation for producing ganoderma lucidum polysaccharide. After ganoderma lucidum submerged fermentation solution culture lasting for 3-5 days, sodium nitroprusside as an inducer is added into a ganoderma lucidum submerged fermentation solution to induce ganoderma lucidum polysaccharide production in ganoderma lucidum submerged fermentation. Sodium nitroprusside crystals as nitric oxide donors are dissolved in water so that a sodium nitroprusside aqueous solution is formed and is used as an inducer for ganoderma lucidum submerged fermentation used for producing ganoderma lucidum polysaccharide. Through slowly releasing nitric oxide in cells, sodium nitroprusside can promote secondary metabolite ganoderma lucidum polysaccharide synthesis. A result of an experimental research shows that through utilization of an appropriate amount of sodium nitroprusside in a ganoderma lucidum fermentation solution, a ganoderma lucidum polysaccharide yield can be effectively increased by 25 to 160%.

Description

technical field [0001] The invention relates to the submerged fermentation technology of ganoderma lucidum, especially the application of sodium nitroprusside (SNP) in ganoderma lucidum polysaccharide, a secondary metabolite of submerged ganoderma lucidum, so as to increase the yield of ganoderma lucidum polysaccharide. Background technique [0002] Ganoderma [ Ganoderma lucidum (Fr.) Krast (Polyporaceae)] belongs to the order Aphylloides (Aphylloides, Aphyllophorales ) Ganoderma lucidum ( Ganodermataceae ) Ganoderma lucidum ( Ganoderma ), known as "immortal grass" and "panacea", is a kind of fungus with both food and medicine, and has a long history of application in China, Japan, Korea and Taiwan. Modern biology has proved that Ganoderma lucidum fruiting bodies and mycelia contain polysaccharides, nucleosides, amino acid proteins, triterpenes, furans, alkaloids, oils, sterols, organic germanium, inorganic ions and other activities Substances, in which Ganoderma luci...

Claims

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Application Information

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IPC IPC(8): C12P19/04
Inventor 王松华李正鹏蒋圣娟祝嫦巍何华奇何庆元孙玉军
Owner ANHUI SCI & TECH UNIV
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