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Method for measuring contents of three flavonoid compounds in rhododendron seniavinii syrup simultaneously

A technology of flavonoids and white syrup, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long time consumption, and achieve the effect of reducing steps and time consumption

Inactive Publication Date: 2012-11-21
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The reported method is the application of high performance liquid chromatography for separate quantitative determination of hyperoside, quercitrin and quercetin, making the time-consuming determination of the content of various flavonoids in Manshan white syrup longer

Method used

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  • Method for measuring contents of three flavonoid compounds in rhododendron seniavinii syrup simultaneously
  • Method for measuring contents of three flavonoid compounds in rhododendron seniavinii syrup simultaneously
  • Method for measuring contents of three flavonoid compounds in rhododendron seniavinii syrup simultaneously

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Preparation of standard solution: Weigh and mix 20mg hyperin, 10mg quercetin, and 10mg quercetin reference substances, and then use methanol to dissolve the mixed reference substances and dilute to obtain a total concentration of 4.000mg / mL of mother liquor, and then diluted the mother liquor into 6 concentration gradients, the concentration gradients were 0.01, 0.1, 0.2, 0.5, 0.8 and 1.0 times of the concentration of the mother liquor; among them, hyperoside, quercetin, and quercetin were compared The purity of the product is 98%.

[0027] 2. Preparation of the test solution: take 18mL syrup and add 6mL water-saturated n-butanol for extraction (that is, the volume ratio of syrup and water-saturated n-butanol is 3:1), extract 3 times in total, combine the extracts, and combine The extract was concentrated to dryness under reduced pressure, then dissolved in methanol and transferred to a 25mL volumetric flask to constant volume (, and then filtered with a 45nm pore...

Embodiment 2

[0041] Preparation of test solution: take 18mL syrup and add 18mL water-saturated n-butanol for extraction (i.e. the volume ratio of syrup and water-saturated n-butanol is 1:1), extract 5 times in total, combine the extracts, and combine The extract was concentrated to dryness under reduced pressure, then dissolved in methanol and transferred to a 25mL volumetric flask to constant volume, and then filtered with a 45nm pore size filter membrane to obtain the extract of flavonoids in Manshanbai syrup, which was the solution to be tested ;

[0042] Determination: Get the liquid to be tested prepared in this embodiment and inject it into a high-performance liquid chromatograph with the same chromatographic conditions as in Example 1 for analysis and determination to obtain the high-performance liquid chromatogram of the liquid to be tested. Specifically, the high-efficiency liquid chromatogram of the liquid to be tested The liquid chromatogram is as image 3 As shown, and the pea...

Embodiment 3

[0044] Preparation of test solution: take 18mL syrup and add 36mL water-saturated n-butanol for extraction (i.e. the volume ratio of syrup and water-saturated n-butanol is 1:2), extract once, and concentrate the extract to dryness under reduced pressure. Then add methanol to dissolve and transfer to a 25mL volumetric flask to constant volume, then filter with a 45nm pore size filter membrane to obtain the extract of flavonoids in Manshanbai syrup, which is the solution to be tested;

[0045] Determination: Get the liquid to be tested prepared in this embodiment and inject it into a high-performance liquid chromatograph with the same chromatographic conditions as in Example 1 for analysis and determination to obtain the high-performance liquid chromatogram of the liquid to be tested. Specifically, the high-efficiency liquid chromatogram of the liquid to be tested The liquid chromatogram is as Figure 4 As shown, and the peak area was obtained by integrating the software of the ...

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Abstract

The invention provides a method for measuring the contents of three flavonoid compounds in rhododendron seniavinii syrup simultaneously. The method comprises the following steps of: firstly preparing standard solution and liquid to be measured, then carrying out high-performance liquid chromatography detection on the standard solution and the liquid to be measured, wherein the chromatographic condition is as follows: the chromatographic column is a C18 chromatographic column; the mobile phase is acetonitrile and water; the column temperature is 25 DEG C; the detection wavelength is 360nm; the sample size is 20muL; the change process of the mobile phase is 0-25min; the volume ratio of the acetonitrile and the water is 70:30; in 25-26min, the volume ratio of the acetonitrile and the water is changed from 70:30 isocratic gradient to 60:40; and in 26-55min, the volume ratio of the acetonitrile and the water is 60:40. The method has the advantages that the contents of the three flavonoid compounds in the rhododendron seniavinii syrup can be measured simultaneously, so that the measurement steps are reduced and the time consumed by measurement is reduced.

Description

【Technical field】 [0001] The invention relates to a method for simultaneous determination of multiple components, in particular to a method for simultaneous determination of the contents of three flavonoids in Manshanbai syrup. 【Background technique】 [0002] Manshanbai is the dry twigs and leaves of Rhododendron senniavinii Maxim., a plant in the Rhododendaceae family. It has the effect of eliminating phlegm and relieving cough. It is a local medicinal material in Fujian Province; Raw materials for clinical application of Chinese patent medicines. The flavonoids such as hyperin, quercetin and quercetin in Manshanbai cough syrup series products are the material basis of their pharmacological activity, and the content levels of hyperin, quercetin and quercetin It directly affects the quality of medicinal raw materials, and ultimately affects the quality of finished medicine. The development and application of a rapid quantitative determination method for flavonoids in Mansh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02
Inventor 黄颖桢陈菁瑛赵云青林峰
Owner 福建省农业科学院农业生物资源研究所
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