Chick embryo culture method for White spot syndrome virus

Abstract

The invention discloses a chick embryo culture method for White spot syndrome virus, which is characterized in that a SPF chick embryo is employed as a in vitro culture system of the White spot syndrome virus, a healthy chick embryo precultured for 6-7 days is selected, the prepared degermd White spot syndrome virus is inoculated to a chick embryo allantois membrane, and the death chick embryo with the culture time of 5-10 days is collected for homogenate and centrifugation to obtain a supernatant containing White spot syndrome virus, the culture method provided by the invention has the advantage that a WSSV in vitro culture method with high efficiency and effective application is provided for rapidly and effectively in vitro subcultring on WSSV, the problems of no appropriate culture system during WSSV culture process and difficult virus subcultring in a current culture method can be overcome, thereby the application of the chick embryo culture method in WSSV can supply the blank of the White spot syndrome virus chick embryo culture system and establish a base for further research and application.

Description

technical field [0001] The invention relates to the research field of culture technology of prawn white spot syndrome virus, in particular to a chicken embryo culture method of prawn white spot syndrome virus. Background technique [0002] Shrimp white spot syndrome virus (White Spot Syndrome Virus, WSSV) is the main pathogen that causes shrimp white spot syndrome. First discovered in Taipei, China in the early 1990s, it is the main viral pathogen that has caused large-scale and frequent outbreaks of artificially cultured prawns in my country's coastal areas since 1993. Similar outbreaks followed in other countries in Asia, as well as countries on the Pacific coast. The virus can cause various typical symptoms in prawns. For example, they do not like to eat, swim back and forth by the pool, their body color turns red, and they are slow to respond to the outside world. Easy to be peeled off, obvious white spots appear on the carapace. [0003] Shrimp white spot syndrome vi...

AI Technical Summary

Problems solved by technology

However, virus chicken embryo culture is more common in the cultivation of some higher animal viruses, and the research and application in the field of aquatic animal viruses is rarely reported.

At present, there is no relevant research report on the method of cultivating shrimp viruses including shrimp white spot syndrome virus using chicken embryo culture method at home and abroad

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