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Tissue culture method for pyrethrum cinerariifolium

A technology of tissue culture and pyrethrum, which is applied in the field of agricultural biotechnology, can solve the problems of not meeting the needs of industrialized planting, low survival rate, slow reproduction speed, etc., and achieve simple and easy management, high survival rate and fast reproduction speed Effect

Active Publication Date: 2013-09-04
YUNNAN NANBAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problems in the prior art that the industrialized cultivation and planting of pyrethrum is carried out by adopting the division method for asexual reproduction, the reproduction speed is slow, the survival rate is low, and the needs of industrialized planting cannot be met. The present invention provides a pyrethrum tissue culture method, which The technical solution includes the following steps:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of pyrethrum tissue culture method, its technical scheme comprises the following steps:

[0023] (a) Selection of flower buds and sterilization: select unopened flower buds of excellent varieties of pyrethrum as materials for tissue culture, the selected flower buds must be robust, disease-free, and pest-free, and the selected flower buds must not have unfolded;

[0024] Put the selected flower buds into running water for 5 minutes, then place the washed flower buds in a sodium hypochlorite solution with a mass percentage concentration of 15% for soaking, and constantly stir and vibrate during the soaking process. The soaking time is 10 minutes. After the soaking is completed, the flower buds are taken out from the sodium hypochlorite solution, and then the flower buds are rinsed with sterile water for 3 times, and the rinsed flower buds are inoculated for later use;

[0025] (b) Callus induction: put the washed flower buds in the ultra-clean workbench, peel off ...

Embodiment 2

[0037] A kind of pyrethrum tissue culture method, its technical scheme comprises the following steps:

[0038] (a) Selection of flower buds and sterilization: select unopened flower buds of excellent varieties of pyrethrum as materials for tissue culture, the selected flower buds must be robust, disease-free, and pest-free, and the selected flower buds must not have unfolded;

[0039] Put the selected flower buds into running water for 10 minutes, then place the washed flower buds in a sodium hypochlorite solution with a mass percentage concentration of 15% for soaking, constantly stir and vibrate during the soaking process, and the soaking time is 20 minutes. After the soaking is completed, the flower buds are taken out from the sodium hypochlorite solution, and then the flower buds are rinsed with sterile water for 5 times, and the rinsed flower buds are inoculated for later use;

[0040] (b) Callus induction: put the washed flower buds in the ultra-clean workbench, peel off...

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Abstract

A tissue culture method for pyrethrum cinerariifolium includes using unopened buds of pyrethrum cinerariifolium as explant material, subjecting the buds to sterilization, inoculating receptacles of the buds to callus induction medium, culturing the receptacles to obtain green callus, transferring the obtained callus to callus differentiation medium, culturing to obtain a seedling clump of pyrethrum cinerariifolium, subjecting the obtained seedling clump of pyrethrum cinerariifolium to aseptic seedling multiplication to obtain mass seedling clumps of pyrethrum cinerariifolium, transferring the seedling clumps of pyrethrum cinerariifolium to rooting medium, culturing to obtain rooted seedlings of pyrethrum cinerariifolium, exercising the obtained rooted seedlings of pyrethrum cinerariifolium, transplanting the seedlings to a field, and growing flowers of pyrethrum cinerariifolium. The seedlings obtained by the tissue culture method for pyrethrum cinerariifolium can retain excellent characters of female parents of pyrethrum cinerariifolium and are fast in breeding and high in survival rate. Hydroponic seedling exercising is simple to operate, management is simple and easy, cost is low, and survival rate of seedling exercising and transplanting is high.

Description

technical field [0001] The invention belongs to the application field of agricultural biotechnology, and relates to a pyrethrum tissue culture method. Background technique [0002] Pyrethrum is a plant belonging to the Compositae family, and its flowers contain pyrethrin, a highly effective insecticidal active substance. In recent years, with the increasing emphasis on food safety and the increasing intensity of regulation in various countries around the world, the planting and processing industry of pyrethrum has developed rapidly. . Planting and cultivation of pyrethrum has two kinds of propagation methods: seed propagation and asexual propagation. Seed propagation cannot maintain the original excellent traits of pyrethrum. Therefore, in the actual industrial planting process, pyrethrum is mainly planted and cultivated by asexual propagation. The commonly used method is the division method, which has slow propagation speed and low survival rate, and cannot meet the needs ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 刘科新朱者舍李仙
Owner YUNNAN NANBAO BIOTECH
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