Propionibacterium jensenii strain and application thereof

A technology for producing Propionibacterium jannaschii and Propionibacterium jannaschii, which can be applied to bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of low propionic acid yield and high propionic acid cost

Active Publication Date: 2012-11-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microbial production of propionic acid still has

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Screening method for propionic acid-producing Propionibacterium janesii

[0026] Take a certain amount of silt, put it into a 250mL Erlenmeyer flask filled with 100mL sterile water and glass beads to dilute different concentration gradients, and put the diluted samples of different concentration gradients into the enrichment medium and let it stand at 30°C Anaerobic culture for 4-5 days, respectively re-transfer fresh enrichment medium 30 ° C static anaerobic culture for 4-5 days.

[0027] The enriched culture solution was serially diluted, and the primary screening medium was respectively coated with anaerobic culture at 30°C for 4-5 days. The colonies were respectively inserted into the re-screening medium, and incubated anaerobically at 30°C for 4-5 days. The bacterial concentration was measured respectively, and 3-4 strains with the highest bacterial concentration were selected to be inserted into the fermentation medium for yield detection. The strain ...

Embodiment 2

[0028] Embodiment 2: Detection of Propionibacterium janesii propionic acid production

[0029] Inoculate the seed medium with 1% inoculum. When it grows to the logarithmic growth phase, inoculate the fermentation medium with 10% inoculum, cultivate anaerobically at 30°C for 150 hours, take the fermentation liquid at 8000rpm, and centrifuge at 4°C 2min. Take the supernatant and dilute to 10mL with 3.68mmol / L sulfuric acid.

[0030] Detection conditions: Agilent HPLC, Agilent ZORBAX SB-Aq liquid phase column, column temperature 35°C, detection wavelength 210nm, injection volume 10μL, mobile phase 19.7g disodium hydrogen phosphate and 10mL acetonitrile dissolved in 1000mL ultrapure water and adjust the pH to 2.0 with phosphoric acid.

[0031] After HPLC detection, the strain with the highest propionate production was named Propionibacterium jensenii JN925, and its propionate production was 20.96 g / L in 150 h of anaerobic bottle fermentation.

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Abstract

The invention discloses a Propionibacterium jensenii strain and application thereof. The Propionibacterium jensenii strain is preserved in the China Center for Type Culture Collection on May 3, 2012, and has a preservation number of CCTCC No.M 2012153. The Propionibacterium jensenii is screened out from sewage sludge from a cheese factory; and yield of propionic acid produced from fermentation bythe Propionibacterium jensenii reaches 20.96g / L.

Description

technical field [0001] The invention relates to a Propionibacterium janschii, in particular to a propionic acid-producing Propionibacterium janschii. Background technique [0002] Propionic acid and its derivatives are widely used in industry, mainly used in food preservation, feed storage, synthesis of pharmaceutical intermediates, synthesis of agricultural herbicides, organic synthesis intermediates, etc. In 2006, the world's total production capacity of propionic acid was about 350,000 tons / year, and in 2008 it reached 500,000 tons / year. The United States is the world's largest producer and consumer of propionic acid. At present, the actual annual output of propionic acid in my country is only about 200 tons, which is far from meeting the actual market demand, and a large amount of imports are still needed to make up for the domestic vacancy. [0003] The strains used for propionic acid fermentation are mainly Propionibacteria, which has multiple species and subspecies,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/52C12R1/01
Inventor 陈坚诸葛鑫刘龙堵国成
Owner JIANGNAN UNIV
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