Cleft lip and palate susceptibility gene detection chip and detection method

Abstract

The invention relates to gene chip, and specifically relates to a cleft lip and palate susceptibility gene detection chip and a detection method employing the chip. The chip comprises a substrate and nucleic acid probes positioned on the substrate. The nucleic acid probes comprise nucleic acids with nucleotide sequences represented by SEQ ID No.1-34. The 3' sites of the probes are subjected to amino modification. The gene chip of the cleft lip and palate susceptibility gene is used for detecting the relationships between various genes and cleft lip and palate. According to the gene chip, probes of sites of all the genes and possible to be related with cleft lip and palate are integrated into one chip. A base complementary hybridization method is adopted, such that high-flux, raft, and accurate detections are realized. With the cleft lip and palate susceptibility gene detection chip, gene screening upon susceptible populations can be rapidly and conveniently carried out, high-risk populations can be detected within early pregnancy or pre-pregnancy periods, such that occurrences of cleft lip and palate can be prevented and reduced through means of reducing the contact with risk factors and improving conditions.

Description

technical field [0001] The invention relates to a gene chip, in particular to a cleft lip and palate susceptibility gene detection chip and a detection method. Background technique [0002] Nonsyndromic cleft with or without cleft palate (NSCL / P) is a relatively common congenital facial defect that does not belong to any syndrome and is not accompanied by other systemic organ abnormalities. One of the most common congenital malformations. The treatment of non-syndromic cleft lip and palate requires the intervention of various disciplines such as surgery, nutrition, dentistry, language, internal medicine, and behavior, which brings a significant economic burden to society and families. The incidence rate of cleft lip and palate of different races in different regions of the world is quite different, with an average of 1 / 700. The incidence rate in my country is relatively high, at 1 / 500. Although the exact etiology of cleft lip and palate is still inconclusive, genetic and e...

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Problems solved by technology

(5) Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in folic acid metabolism, and the mutation of C677T encoding this enzyme will lead to heat intolerance and reduced activity of the enzyme

[0005]Based on the fact that the above-mentioned genes may be related to the occurrence of cleft lip and palate, there is no research involving the use of gene chip methods to detect cleft lip and palate susceptible population at home and abroad

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