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Microecological preparation for aquaculture and its preparation method

A micro-ecological preparation, aquaculture technology, applied in the direction of animal feed, fungi, animal feed, etc., can solve the problems such as the inability to play the synergistic effect of different strains, hypoxia, and single strain.

Active Publication Date: 2010-07-28
无锡中顺生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing microbial products are aerobic products, which must be used in conjunction with the use of oxygen-enhancing products, otherwise it will cause hypoxia.
Moreover, the strains in the existing microbial products are generally relatively single, and the synergistic effect between different strains cannot be well exerted.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment one: among the present invention, liquid culture medium formula ratio is by weight percentage (liquid culture medium W / W):

[0030] 1. Preparation of Acetobacter preparation

[0031] Take 5% glucose, 2% yeast extract, 93% water, and sterilize at 121°C for 30 minutes to form a sterilized medium; on the above-mentioned sterilized medium, inoculate with 1% V / V inoculum to activate Acetobacter acetobacter was fermented in a 1-ton fermenter at 30°C for 48 hours to obtain an Acetobacter preparation.

[0032] 2. Preparation of Bacillus subtilis preparation

[0033] Take 2% glucose, 1% peptone, 0.2% yeast extract, 0.2% KH 2 PO 4 , 0.2% NaNO 3 , 0.2% NaCl, 0.02% MgSO 4 , 96.18% water, sterilized at 121°C for 30 minutes as a sterilized medium; on the above-mentioned sterilized medium, insert activated Bacillus subtilis with 1% V / V inoculum, and ferment in 1 ton Ferment in a tank at 35°C for 24 hours to obtain a Bacillus subtilis preparation.

[0034] 3. Preparat...

Embodiment 2

[0039] Embodiment two: among the present invention, liquid culture medium formula ratio is by weight percentage (liquid culture medium W / W):

[0040] 1. Preparation of Acetobacter preparation

[0041] Take 7% glucose, 3% yeast extract, and 90% water, and sterilize at 121°C for 30 minutes to form a sterilized medium; on the above-mentioned sterilized medium, insert the activated medium with a 3% V / V inoculum Acetobacter acetobacter was fermented in a 1-ton fermenter at 32°C for 60 hours to obtain an Acetobacter preparation.

[0042] 2. Preparation of Bacillus subtilis preparation

[0043] Take 1% glucose, 2% peptone, 0.3% yeast extract, 0.3% KH 2 PO 4 , 0.3% NaNO 3 , 0.3% NaCl, 0.03% Mg5O 4 , 95.77% water, sterilized at 121°C for 30 minutes; on the above-mentioned sterilized medium, insert activated Bacillus subtilis with 3% V / V inoculum, and in a 1 ton fermenter at 38°C Ferment for 30 hours to obtain a Bacillus subtilis preparation.

[0044] 3. Preparation of Bacillus thu...

Embodiment 3

[0049] Embodiment three: among the present invention, liquid culture medium prescription ratio is by weight percentage (liquid culture medium W / W):

[0050] 1. Preparation of Acetobacter preparation

[0051] Take 10% glucose, 5% yeast extract, and 85% water, and sterilize at 121°C for 30 minutes; on the above-mentioned sterilized medium, insert activated Acetobacter at a 5% V / V inoculum, and in 1 Ferment at 35°C for 72 hours in a 1-ton fermenter to obtain the Acetobacter preparation.

[0052] 2. Preparation of Bacillus subtilis preparation

[0053] Take 5% glucose, 5% peptone, 0.6% yeast extract, 0.6% KH 2 PO 4 , 0.6% NaNO 3 , 0.6% NaCl, 0.06% MgSO 4 , 87.54% water, sterilized at 121°C for 30 minutes; on the above-mentioned sterilized medium, insert activated Bacillus subtilis with a 5% V / V inoculum, and in a 1 ton fermenter at 40°C Ferment for 36 hours to obtain a Bacillus subtilis preparation.

[0054] 3. Preparation of Bacillus thuringiensis preparation

[0055] Tak...

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Abstract

The present invention relates to a microecological preparation for aquaculture and its preparation method. It is characterized by that said preparation method includes the following steps: firstly, respectively preparing acetobacter preparation, bacillus subtilis preparation, bacillus thuringiensis preparation and clostridium preparation, then uniformly mixing them according to a certain mixing ratio and using acetic acid to regulate pH value so as to obtain the invented microecological preparation for aquaculture.

Description

technical field [0001] The invention relates to a microecological preparation for aquaculture and a preparation method thereof, belonging to the technical field of biological bacteria preparations. Background technique [0002] In recent years, with the continuous expansion of the scale of artificial breeding, the breeding environment has become increasingly deteriorating, and disease outbreaks have become more frequent, which has greatly inhibited the growth of aquaculture production and the development of aquatic trade. The application of antibiotics as feed additives began in the 1940s, and has played a positive role in inhibiting the reproduction of pathogenic microorganisms and reducing the toxic effects of their metabolic toxins on animals. However, the long-term use of antibiotics, especially the abuse of antibiotics, has gradually revealed its various disadvantages. For example, it leads to the imbalance of normal flora in the gastrointestinal tract of animals, adve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/16A23K1/18C12N1/14A23K10/18A23K50/80
Inventor 黄忠平
Owner 无锡中顺生物技术有限公司
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