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Liver cancer serum nucleic acid aptamers

A serum nucleic acid and aptamer technology, applied in the field of biomedical detection and analysis, can solve problems such as unsatisfactory sensitivity and specificity, and achieve the effect of good application prospects

Inactive Publication Date: 2014-04-09
NANCHANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Alpha-fetoprotein (AFP) is currently the most widely used serum marker for liver cancer in the world. It has been used for decades, but its sensitivity (40%-65%) and specificity (76%-96%) are not ideal

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  • Liver cancer serum nucleic acid aptamers
  • Liver cancer serum nucleic acid aptamers
  • Liver cancer serum nucleic acid aptamers

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Embodiment Construction

[0039] The following specific examples will further illustrate the present invention, that is, the use of neutral polyacrylamide gel electrophoresis and grayscale analysis methods to apply the liver cancer serum nucleic acid aptamer AP-HCS-9-74 to the detection of liver cancer and non-liver cancer serum samples , and evaluate its diagnostic value for liver cancer.

[0040] 1. Collection and processing of blood samples: collect peripheral venous blood from healthy people who come to the First Affiliated Hospital of Nanchang University with a clear diagnosis of primary liver cancer, liver cirrhosis, and viral hepatitis B and who have participated in physical examinations and all examinations are normal. For non-anticoagulated specimens, after natural coagulation, the serum was routinely centrifuged and stored at -80°C for later use. 50 μl of serum samples from 20 cases of liver cancer were randomly collected and mixed well to prepare mixed serum of liver cancer, which was stored...

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Abstract

The invention discloses liver cancer serum nucleic acid aptamers, belonging to the technical field of biomedical detection and analysis. The invention discloses nine nucleic acid sequences of the liver cancer serum nucleic acid aptamers screened by using the liver cancer serum as a target, and the combination between the nucleic acid sequences and liver cancer and non-liver cancer serum samples is analyzed by polyacrylamide gel electrophoresis and gray level determination. According to the invention, each nucleic acid aptamer has good diagnostic value for liver cancer, the area under a receiver operating characteristic curve is 0.894-0.949, and the accuracy for identifying liver cancer patients and non-liver cancer patients is 84.4-94.4%. The nucleic acid aptamers can be directly applied in the analysis of the liver cancer serum sample or applied after modification, provides experimental basis for clinic diagnosis, treatment and prognosis of liver cancer, and can be applied in establishing new technology of liver cancer detection based on the nucleic acid aptamers. The invention has good application value.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection and analysis. Background technique [0002] Nucleic acid aptamer (aptamer) is an artificial single-stranded oligonucleotide ligand of a biological target molecule, which is screened from a random single-stranded oligonucleotide library by SELEX (Systematic Evolution of Ligands by Exponential Enrichment) technology. The basic steps of nucleic acid aptamer screening are as follows: design and synthesize a random single-stranded oligonucleotide library; co-incubate the library with the target, so that the oligonucleotide in the library that can specifically bind to the target forms a complex with the target, and then separates the complex And elute the oligonucleotides, use the eluted oligonucleotides as templates for PCR amplification, prepare a new single-stranded random oligonucleotide library, and start the next round of screening; repeat several rounds of incubation-elution-amplific...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/115C12Q1/68
Inventor 张焜和陈文学徐国峰王婷魏淑琴李璇吕农华张吉翔
Owner NANCHANG UNIV
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