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Cefradine molecule imprinted membrane preparation method and application

A molecularly imprinted membrane, cefradine technology, used in chemical instruments and methods, membrane technology, semi-permeable membrane separation and other directions

Inactive Publication Date: 2013-11-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the preparation of cephradine molecularly imprinted membrane and the method of using it to analyze and detect cephradine have not been reported

Method used

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  • Cefradine molecule imprinted membrane preparation method and application
  • Cefradine molecule imprinted membrane preparation method and application
  • Cefradine molecule imprinted membrane preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1. A method for preparing cefradine molecularly imprinted membrane, followed by the following steps:

[0069] 1) Preparation of polyacrylonitrile base film by phase inversion method:

[0070] Weigh 5g polyacrylonitrile and dry it in vacuum at 60°C for 24 hours, add 1g lithium chloride to 26.5ml N-methylpyrrolidone, and magnetically stir overnight (12 hours) at 60°C to make the polyacrylonitrile and chlorinated Lithium is fully dissolved.

[0071] The resulting solution was filtered and degassed, and allowed to stand at 60°C for 2 hours. Use a 200 μm spatula to scrape the film on the glass plate to control the thickness of the film to 200 μm. After staying in the air for a certain period of time (1 to 5 minutes), the glass plate Immerse in deionized water at a certain temperature (20~30 ℃). After the film is formed, it is soaked in deionized water for 36 h at room temperature, and the deionized water is changed once in between to obtain the soaked polyacrylonitrile-ba...

Embodiment 2

[0078] Example 2. A method for determining the concentration of cefradine in a liquid test product, the following steps are performed in sequence:

[0079] 1). Draw the standard curve and proceed with the following steps:

[0080] ①. The fluorescein is prepared with a pH 5.8 phosphate buffer to a concentration of 1×10 -6 mol / L fluorescein solution;

[0081] ②. Add a certain amount (or no addition) of cefradine solution (with deionized water as the solvent) into 7 volumetric flasks, add 2ml fluorescein solution to each volumetric flask, and then dilute to 10ml with deionized water ; So as to prepare a series of cefradine standard solutions with cefradine concentrations of 0, 0.5, 1, 2, 3, 4, and 5 mg / L; immediately perform the following step ③ after preparation.

[0082] ③. Detect the fluorescence value of the above series of cefradine standard solutions at the excitation wavelength of 484nm and emission wavelength of 512nm by using the fluorescence method. Use the concentration of cef...

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Abstract

The invention discloses a preparation method of a cefradine molecule imprinted membrane, which comprises the following steps: 1) using a phase inversion method to prepare a polyacrylonitrile basal film; 2) preparing the cefradine molecule imprinted membrane: taking cefradine as a template molecule, taking methacrylic acid as a functional monomer, taking ethylene glycol dimethacrylate as a cross-linking agent; taking azodiisobutyronitrile as an initiator; using cefradine, tetrabutylammonium hydroxide and methanol to prepare a filtrate; adding methacrylic acid, ethylene glycol dimethacrylate and azodiisobutyronitrile in the filtrate, uniformly stirring to obtain a membrane liquid; putting the obtained polyacrylonitrile basal film in the membrane liquid for immersing, then performing polymerization and elution in order to obtain the cefradine molecule imprinted membrane. The cefradine molecule imprinted membrane can used for determining the concentration of cefradine in a liquid state sample to be measured. The invention also provides a method for determining the concentration of the liquid state sample to be measured in cefradine.

Description

Technical field [0001] The invention relates to the preparation and application of a cefradine molecularly imprinted membrane. Background technique [0002] Cefrading (Cephrading, Velosef), also known as cephalosporin VI, cephalosporin VI, etc., is the first generation of semi-synthetic cephalosporin, with wide antibacterial range, good oral absorption, high blood concentration, and is characterized by β-lactamase resistance , It has a rapid and reliable sterilization effect on drug-resistant Staphylococcus aureus and other bacilli resistant to spectrum antibiotics. It is mainly excreted through urine in its original form, with a higher concentration in urine. Clinically, it is mainly used for infections of the respiratory tract, urinary tract, skin and soft tissues. The 2005 edition of the Chinese Pharmacopoeia uses HPLC to determine the content of cefradine capsules. The current literature reports include spectrophotometry, chemiluminescence, high-performance capillary electr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/26C08J7/16C08L33/20B01D71/78B01D67/00G01N21/64
Inventor 沈生荣于海宁曾思敏
Owner ZHEJIANG UNIV
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