Preparation method of cloxacillin molecular imprinting polymer membrane

A technology of cloxacillin and molecular imprinting, applied in chemical instruments and methods, other chemical processes, etc., can solve the problems that the preparation of cloxacillin molecularly imprinted membranes has not been reported yet, and achieve easy handling and application, low testing cost, and high long lasting effect

Inactive Publication Date: 2015-07-01
XI AN JIAOTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the preparation of cloxacillin molecularly imprinted membrane has not been reported

Method used

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  • Preparation method of cloxacillin molecular imprinting polymer membrane
  • Preparation method of cloxacillin molecular imprinting polymer membrane
  • Preparation method of cloxacillin molecular imprinting polymer membrane

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preparation example Construction

[0026] The preparation method of the cloxacillin molecularly imprinted polymer membrane of the present invention comprises the following steps:

[0027] (1) Preparation of Cloxacillin Polymer Solution

[0028] Weigh cloxacillin and dissolve it in the porogen, ultrasonically, until the cloxacillin is completely dissolved to obtain 20-100 mg / ml cloxacillin organic solution;

[0029] In the above-mentioned cloxacillin organic solution, add the functional monomer, wherein the molar ratio of the template molecule (cloxacillin) to the functional monomer is 1:2-1:12; then vortex for 5-20min to make the cloxacillin Sufficient pre-polymerization of penicillin and functional monomers; then add cross-linking agent (the degree of cross-linking is 60-90%) and initiator (the ratio of initiator to functional monomer is 1:10-1:20) in the solution After being mixed uniformly, the required cloxacillin polymerization solution is obtained; the porogen is dimethylacetamide DMAC, N-methylpyrrolido...

Embodiment 1

[0034] (1), preparation of cloxacillin polymer solution:

[0035] Weigh 170mg of cloxacillin into 7ml of DMF, ultrasonically dissolve at 60°C, then add 0.25μl MAA and vortex for 20min to fully pre-polymerize cloxacillin and functional monomers, then add 1.5ml of EDMA, 66mg of TPO and vortex Make it evenly mixed, and then pass nitrogen gas for 10 minutes to remove oxygen to obtain cloxacillin polymerization solution.

[0036] (2) Preparation of cloxacillin molecularly imprinted polymer membrane:

[0037] Immerse the commercialized PVDF in the above polymerization solution, continue to pass nitrogen gas in the solution for 10 minutes to remove oxygen, and then seal it; after standing for 20 minutes, transfer the soaked PVDF to a closed container that has been deoxygenated; The reaction was irradiated for 4 hours; then step (2) was repeated three times.

[0038] The membrane obtained from the above polymerization was eluted with a mixture of methanol and glacial acetic acid (me...

Embodiment 2

[0041] (1), preparation of cloxacillin pre-polymerization solution:

[0042] Weigh 170.4mg cloxacillin into 7.5ml DMSO, dissolve it by ultrasonic at 50℃, then add 0.35ml MAA and vortex for 10min to fully pre-polymerize cloxacillin and functional monomer, then add 1.8ml TRIM, 100mg AIBN and vortex Rotate to mix well, and then pass nitrogen gas for 5 minutes to remove oxygen to obtain cloxacillin polymer solution.

[0043] (2) Preparation of cloxacillin molecularly imprinted polymer membrane:

[0044] Immerse the commercialized PVDF membrane in the above-mentioned cloxacillin polymerization solution, continue to pass nitrogen in the solution for 5 minutes to remove oxygen, and then seal it; Irradiate and react under the cross-linking instrument for 12 hours; then repeat step (2) once more.

[0045] The membrane obtained from the above polymerization was eluted with a mixture of methanol and acetic acid (methanol / glacial acetic acid = 1 / 1, v / v) under Soxhlet reflux until no tem...

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Abstract

The invention discloses a preparation method of a cloxacillin molecular imprinting polymer membrane. The preparation method comprises the following steps: (1) preparing a cloxacillin polymerization solution; and (2) preparing a cloxacillin molecular imprinting polymer membrane: introducing nitrogen into the cloxacillin polymerization solution to remove oxygen, soaking a polyvinylidene fluoride membrane into the cloxacillin polymerization solution, introducing nitrogen to remove oxygen, standing and soaking; taking out the completely soaked polyvinylidene fluoride membrane, placing the completely soaked polyvinylidene fluoride membrane into a vessel which is subjected to oxygen removal, and reacting under an ultraviolet crosslinking instrument; taking out the membrane obtained after complete reaction, eluting the membrane by using a mixed liquid of methanol and glacial acetic acid until template molecules cannot be detected in eluent, and washing the membrane by using an acetonitrile aqueous solution until the pH value is neutral to obtain the cloxacillin molecular imprinting polymer membrane. The preparation method of the cloxacillin molecular imprinting polymer membrane has important significance in cloxacillin residual detection on animal-derived food. The cloxacillin molecular imprinting polymer membrane prepared by the preparation method has the characteristics of simplicity and convenience in operation, low energy consumption, short reaction time, cleanness, no pollution and the like.

Description

technical field [0001] The invention belongs to the technical field of drug testing, and relates to a polymeric film for testing cloxacillin, in particular to a molecularly imprinted polymeric film for cloxacillin. Background technique [0002] Cloxacillin (CLOX), also known as cloxacillin, is a broad-spectrum semi-synthetic penicillin with the characteristics of acid resistance and penicillinase resistance. The antibacterial mechanism is to inhibit the synthesis of cell walls, and it has antibacterial activity against Gram-positive cocci and Neisseria . Clinically, it is mainly used to treat various serious infections caused by penicillinase-producing staphylococci, such as sepsis, osteomyelitis, respiratory tract infection, endocarditis and septic arthritis. [0003] However, in recent years, with the wide application of antibiotics, cloxacillin has been widely used in aquaculture to treat diseases caused by bacteria in aquatic products such as fish, shrimp, and sea cucum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/12C08J9/28C08L27/16C08F220/06C08F226/06C08F222/14B01J20/26B01J20/30
Inventor 傅强孙敏杜玮杜康丽蔡刚常春
Owner XI AN JIAOTONG UNIV
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