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Poly(DL-lactic acid) material modified based on 24 peptides in E domain of mechano-growth factor (MGF), and preparation method and application thereof

A technology of polylactic acid materials and growth factors, applied in medical science, surgery, prostheses, etc., can solve problems not specified in detail, achieve good hydrophilicity and cell affinity, relieve inflammation, promote blood vessel and bone The effect of tissue regeneration

Inactive Publication Date: 2012-10-17
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application did not specify in detail how the material can be used as a medical biomaterial, and how to further introduce polypeptides

Method used

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  • Poly(DL-lactic acid) material modified based on 24 peptides in E domain of mechano-growth factor (MGF), and preparation method and application thereof
  • Poly(DL-lactic acid) material modified based on 24 peptides in E domain of mechano-growth factor (MGF), and preparation method and application thereof
  • Poly(DL-lactic acid) material modified based on 24 peptides in E domain of mechano-growth factor (MGF), and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 Cyclic anhydride modified polylactic acid

[0041]5.0 g of poly(D,L-lactic acid), 0.5 g of maleic anhydride, and 10 mg of tert-butyl peroxide were mixed uniformly, and vacuum melted and sealed in a 50 ml round bottom flask. React at 50°C for 24 hours to obtain brown-yellow maleic anhydride-modified polylactic acid. The product was dissolved in dichloromethane and dropped into excess ether, and the precipitate was collected. Repeat to dissolve the pellet three times. The precipitate was placed under vacuum for 48 hours at room temperature to obtain 3.8 g of brown maleic anhydride-modified polylactic acid, the molecular formula of which was as follows: Said A is D, L-lactic acid; through parallel tests: when A is D-lactic acid or L-lactic acid, it can all be realized.

[0042] Maleic acyl-modified polylactic acid is a kind of cyclic acid acyl-modified polylactic acid. For other cyclic acyl-modified polylactic acid and its preparation method, please refer...

Embodiment 2

[0043] Embodiment 2 Cyclic anhydride modified polylactic acid

[0044] 5.0 g of poly(D,L-lactic acid) (wherein n=100) and 0.5 g of maleic anhydride and 20 mg of tert-butyl peroxide were uniformly mixed, vacuum melted and sealed in a 50 ml round bottom flask. React at 50°C for 24 hours to obtain brown-yellow maleic anhydride-modified polylactic acid. The product was dissolved in dichloromethane and dropped into excess ether, and the precipitate was collected. Repeat to dissolve the pellet three times. The precipitate was irritated under vacuum at room temperature for 48 hours to obtain 3.5 grams of brown maleic anhydride-modified polylactic acid, the molecular formula is as follows: Said A is D, L-lactic acid; through parallel tests: when A is D-lactic acid or L-lactic acid, it can all be realized.

Embodiment 3

[0045] Embodiment 3 Cyclic anhydride modified polylactic acid

[0046] 5.0 g of poly(D,L-lactic acid), 0.5 g of maleic anhydride, and 30 mg of tert-butyl peroxide were evenly mixed, and vacuum melted and sealed in a 50 ml round bottom flask. React at 50°C for 24 hours to obtain brown-yellow maleic anhydride-modified polylactic acid. The product was dissolved in dichloromethane and dropped into excess ether, and the precipitate was collected. Repeat to dissolve the pellet three times. Put the precipitate under vacuum at room temperature for 48 hours to obtain 4.2 g of brown maleic anhydride-modified polylactic acid, the molecular formula is as follows The A is D, L-lactic acid. In addition, a parallel test is carried out, and it can be realized when A is D-lactic acid or L-lactic acid.

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PUM

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Abstract

The invention relates to a polypeptide modified poly(DL-lactic acid) material. The molecular formula of the poly(DL-lactic acid) material is represented by I or II; and in the formula, A is one selected from the group consisting of D-lactic acid, L-lactic acid and D,L-lactic acid, n is equal to 100 to 20000, and Ct24E-MGF is 24 peptides in the E domain of MGF and has an amino acid sequence as represented by SEQ ID NO. 1. A preparation method for the polypeptide modified poly(DL-lactic acid) material is as follows: maleic anhydride modified poly(DL-lactic acid) having a molecular formula as represented by III and the 24 peptides in the E domain of MGF represented by SEQ ID NO. 1 are used as raw materials, DCC, EDC and NHS are used as condensing agents, then the raw materials and the condensing agents are subjected to a reaction at a temperature of 0 to 50 DEG C for 8 to 48 h, an obtained reaction solution is added into an excess aqueous medium, a membrane-like precipitate is collected, and the membrane-like precipitate is the poly(DL-lactic acid) material modified based on the 24 peptides in the E domain of MGF. Compared to poly(DL-lactic acid) materials which are not modified by polypeptide, the poly(DL-lactic acid) material provided in the invention has better hydrophilicity and cellular affinity and can activate growth of osteoblasts and vascular endothelial cells and promote regeneration of blood vessels and bone tissue.

Description

technical field [0001] The invention belongs to the field of biological materials, in particular to polypeptide modified polylactic acid materials. Background technique [0002] Mesotropins are stress-sensitive growth factors. Produced by alternative splicing of the Igf-1 gene in tissues such as bones stimulated by stress, it has the function of stimulating cells to express extracellular matrix and strengthening the matrix around cells. Under stress-deficient conditions, it is expected to replace the role of stress in stimulating cells to express extracellular matrix. 24 peptides of its carboxy-terminal E domain (tyrosine-glutamine-proline-proline-serine-threonine-asparagine-lysine-asparagine-threonine-lysine acid-serine-glutamine-arginine-arginine-lysine-glycine-serine-threonine-phenylalanine-glutamic acid-glutamic acid-histidine-lysine) has Promote muscle hypertrophy, proliferation of osteoblasts and vascular endothelial cells, and repair damaged functions. Therefore, ...

Claims

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Application Information

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IPC IPC(8): C08G63/91A61L27/18A61L31/06
Inventor 王远亮李玉筱张兵兵潘君王品品
Owner CHONGQING UNIV
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