Naturally-emulsified egg film fresh-keeping agent and its preparation method
A film-coating preservative and emulsifying technology, applied in the field of food processing, to prevent spoilage, good taste and appearance, and maintain internal quality
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Embodiment 1
[0031] Embodiment 1: the preferred test of preservative
[0032] 1.1 Materials and Instruments
[0033] 1.1.1 Source of bacteria and preparation of bacteria solution
[0034] Escherichia coli and Salmonella were provided by the Microbiology Laboratory of the School of Food Science and Technology, Huazhong Agricultural University.
[0035] Firstly, the strains to be tested need to be activated for 1-2 times, then pick 1-2 rings of activated bacteria to be tested in 10.00mL sterile normal saline, and mix well to make a bacterial suspension. In a sterile environment, use a sterile pipette to take 1.00mL of bacterial suspension into the next 9.00mL of sterile normal saline, and make a series of bacterial suspensions in sequence according to this method. Finally, the concentration of the bacterial suspension was adjusted to contain bacterium 10 6 ~10 7 pc / mL for later use.
[0036] 1.1.2 Preparation of reagents, drugs and media
[0037] Eucalyptus leaf essential oil, perilla ...
Embodiment 2
[0069] Embodiment 2: the preferred test of film-forming agent
[0070] 2.1 Materials and Instruments
[0071] 2.1.1 Materials and reagents
[0072] Fresh eggs: Fresh eggs laid within 2 days were purchased from the market of Huazhong Agricultural University.
[0073] Sodium alginate, pullulan, xanthan gum, Tween-80, Span-80, sucrose fatty acid ester, chemically pure, Sinopharm Chemical Reagent Co., Ltd.; corn oil, food grade, Xinyuan Grain and Oil (Wuhan) Ltd.
[0074] 2.1.2 Main instruments
[0075] Constant temperature and humidity chamber, Ningbo Haishu Saifu Experimental Instrument Factory; electric heating constant temperature water bath, Tianjin Test Instrument Co., Ltd.; egg washing machine, Shenzhen Zhenye Membrane Steel Industry Co., Ltd.; magnetic stirrer, Shanghai Sile Instrument Co., Ltd. Electronic balance, Yutao City Jinnuo Balance Instrument Co., Ltd.; Huff unit measuring instrument, Jiangsu Poultry Science Research Institute; acidity meter, Shanghai Precisio...
Embodiment 3
[0113] A natural emulsified poultry egg coating preservative, consisting of the following ingredients in mass percentage:
[0114]
[0115] The film-forming agent is xanthan gum;
[0116] Described emulsifier is sucrose fatty acid ester, Tween-80, Span-80, and its mass ratio is successively 10.5: 6.2: 18.3;
[0117] The preservatives are perilla leaf essential oil, cinnamon essential oil and wintergreen essential oil, the mass ratio of which is 3:6:4 in turn, and the perilla leaf essential oil, cinnamon essential oil and wintergreen essential oil are extracted by distillation.
[0118] The preparation method is as follows: weighing xanthan gum, sucrose fatty acid ester, Tween-80, and Span-80 respectively in proportion, adding them into water, stirring continuously at 60-80°C to fully dissolve them, and then cooling to room temperature; Then take corn oil, perilla leaf essential oil, cinnamon essential oil, and wintergreen essential oil, add them to the prepared solution, emu...
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