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Bacillus amyloliquefaciens B-1619 strain and application in preventing and controlling soil-borne disease of nightshade vegetables thereof

A technology of Bacillus amyloliquefaciens and B-1619, which is applied to Bacillus amyloliquefaciens strain B-1619 and its application field in preventing and controlling soil-borne diseases of Solanaceae vegetables, so as to achieve non-environmental pollution, human and animal safety, and good control effects. Effect

Inactive Publication Date: 2013-10-09
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the use of Bacillus amyloliquefaciens to control soil-borne diseases of Solanaceae vegetables in facilities

Method used

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  • Bacillus amyloliquefaciens B-1619 strain and application in preventing and controlling soil-borne disease of nightshade vegetables thereof

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Experimental program
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Effect test

Embodiment 1

[0026] Isolation and preservation of Bacillus amyloliquefaciens B-1619 strain

[0027] In December 2008, the B-1619 strain was isolated by gradient dilution method in the tomato greenhouse in Shuyang facility in Suqian area. (Gradient dilution separation method: collect soil from the tomato greenhouse in Shuyang facility, weigh 10g and add it to a triangular flask filled with 90mL sterilized water, shake and cultivate for 30min (150r / min), and make a 1:10 (soil: water) suspension, and then take 1mL of the suspension and place it in 9mL sterile water to make 10 -2 Dilute the suspension, and so on, to make 10 -5 , 10 -6 Dilute the suspension; draw 200 μL each, add it to the YPGA medium plate, spread it evenly with a coating bar, and repeat each treatment 3 times. Place the above petri dishes in a constant temperature incubator at 28°C for 1-2 days. Select a single colony and transfer it to the YPGA plate for culture. After the colony grows, use an inoculation loop to streak ...

Embodiment 2

[0031] Preparation of Bacillus amyloliquefaciens B-1619 Biocontrol Bacteria Liquid

[0032] 1. Preparation of Bacillus amyloliquefaciens B-1619 biocontrol strain

[0033] Strain activation: transplant the freeze-dried and preserved B-1619 strain to YPGA medium, 28 o C for 48 hours.

[0034] Preparation of YPGA medium: Weigh 5.0g of yeast extract, 5.0g of tryptone; 5.0g of glucose, 17.0g of agar; put it into 1000ml of water, heat and mix well, then adjust the pH to 6.8-7.2, and then package it in In the test tube, the filling capacity is 1 / 5-1 / 6 of the test tube, and the test tube mouth is plugged with a stopper, 121 o C. Wet heat sterilization for 30 minutes, take out the test tube while it is hot and set it on a slope until it cools down for later use.

[0035] Preparation of fermented strains: Take a ring of activated B-1619 bacteria and transfer them into 300ml YPG culture solution packed in a 1000ml Erlenmeyer flask, at 150rpm, 28 o After cultivating at C for 48 ho...

Embodiment 3

[0046] Preparation of Bacillus amyloliquefaciens B-1619 biocontrol bacteria powder

[0047] The bacterial content that embodiment 2 obtains through fermentation is 10 10 Cfu / mL biocontrol bacteria solution was centrifuged, the supernatant was discarded, the precipitate was taken out and calcium bicarbonate was adsorbed, fully stirred and mixed, and passed through a jet mill to make the biocontrol bacteria powder uniform and form Bacillus amyloliquefaciens B-1619 bio-control bacteria powder; wherein, the centrifugation conditions are: 7000r / min, 10 minutes, the weight ratio of bio-control bacteria liquid and calcium bicarbonate is 1:2.

[0048] The bacterial content of the biocontrol bacteria powder is at least 4×10 9 cfu / mL.

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Abstract

The invention relates to a bacillus amyloliquefaciens B-1619 strain and application in preventing and controlling soil-borne disease of nightshade vegetables thereof. The preservation number of the strain is CGMCC No. 4671. When the strain is applied, biocontrol strain powder is mixed with soil before seeding, and soil with biocontrol strain is covered on seeds after seeding; the biocontrol strain powder is firstly planted on the surface of the soil before seed separation; diluted biocontrol strain liquid is aligned to seeds of nightshade vegetables to be planted before planting, and the diluted biocontrol strain liquid is irrigated to the roots of the plants of nightshade vegetables during the planting; the diluted biocontrol strain liquid is irrigated to the roots of the plants of nightshade vegetables periodically after the planting; or, the application ways in each period from the seeding to the harvesting period of the nightshade vegetables are combined.

Description

technical field [0001] The present invention relates to a strain of Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) B-1619 strain, and the biocontrol agent prepared by using the strain and the application of the agent. The invention belongs to the field of biological control of facility solanaceous vegetables by using beneficial microorganisms. Background technique [0002] The status and role of protected vegetables in my country's agricultural and rural economic development is becoming more and more important, and it has become a pillar industry in modern agricultural production and the main way for farmers to increase their income. The high-yield and continuous cropping facility vegetable planting layout leads to frequent outbreaks of soil-borne diseases of facility vegetables. In addition, most of the current vegetable bases are improved from traditional rice and wheat land, and the soil is severely efflorescent, which aggravates the prevalence of soil-borne d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01G1/00A01G7/06A01G13/00A01N63/00A01P1/00C12R1/07
Inventor 陈志谊刘邮洲乔俊卿聂亚峰
Owner JIANGSU ACAD OF AGRI SCI
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