Primer used for performing pyrophosphoric acid detection on PvuII and XbaI polymorphism of intron 1 of estrogen receptor gene alpha

Abstract

The invention discloses a primer used for performing pyrophosphoric acid detection on PvuII and XbaI polymorphism of an intron 1 of an estrogen receptor gene alpha. The primer comprises a specific primer and a primer, wherein the specific primer is used for amplifying the PvuII and XbaI nucleic acid fragment of the estrogen receptor gene alpha from a sample nucleic acid, and the primer is used for performing pyrophosphoric acid sequencing on the obtained nucleic acid fragment. By adopting the pyrophosphoric acid sequencing technology, through the specific amplification primer and the pyrophosphoric acid sequencing primer disclosed by the invention, the PvuII and XbaI polymorphism of the intron 1 of the estrogen receptor gene alpha, which is related with hormone-dependent diseases, can be detected. The primer used for performing the pyrophosphoric acid detection on the PvuII and XbaI polymorphism of the intron 1 of the estrogen receptor gene alpha is high in specificity and accuracy, and can be clinically used as an auxiliary index and screening of early prevention and early diagnosis on agnogenic hypomenorrhea and other certain hormone-dependent related diseases.

Description

technical field [0001] The invention belongs to the field of life science and biotechnology, and in particular relates to a primer sequence for the detection of polymorphic pyrophosphate in intron 1 P vuII and X baI of estrogen receptor alpha gene. Human estrogen receptor α gene polymorphism is detected with good specificity and high accuracy. Background technique [0002] Hypomenorrhea in young women is a common symptom in gynecology. Its hazard is to affect the implantation of fertile eggs in the endometrium, leading to repeated miscarriages and infertility, which are the prodromal symptoms of amenorrhea. Estrogen is the main hormone that regulates menstruation, but it is found clinically that a group of patients have normal estrogen levels and no medical, surgical or congenital diseases. We call it unexplained hypomenorrhea. At the same time, we performed hysteroscopy on a large number of patients with hypomenorrhea for unknown reasons, and found that many of the patien...

AI Technical Summary

Problems solved by technology

This technology is the first generation of DNA molecular marker technology, using restriction endonuclease to recognize the specific sequence of DNA molecule, and cut the DNA molecule at the specific sequence, that is, to generate the characteristic of restriction fragment, the defect of this method is the detection sensitivity Low and can only detect the approximate type, not specific to the mutation ratio

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