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Application of lophatherum gracile total flavonoids in preparation of medicine and functional food for preventing and treating myocardial ischemia disease

A technique for myocardial ischemia and pale bamboo leaves is applied in the directions of cardiovascular system diseases, drug combinations, medical preparations containing active ingredients, etc., and can solve the problems of the physiological and pharmacological activities of total flavonoids of pale bamboo leaves on myocardial ischemia diseases, etc. Achieve the effect of inhibiting platelet aggregation, many types, and good application prospects

Active Publication Date: 2014-06-04
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no research report on the physiological and pharmacological activity of total flavonoids from the leaves of dandelion on myocardial ischemia

Method used

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  • Application of lophatherum gracile total flavonoids in preparation of medicine and functional food for preventing and treating myocardial ischemia disease
  • Application of lophatherum gracile total flavonoids in preparation of medicine and functional food for preventing and treating myocardial ischemia disease
  • Application of lophatherum gracile total flavonoids in preparation of medicine and functional food for preventing and treating myocardial ischemia disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of total flavonoids of leaves of Pale bamboo, which is prepared by the following method:

[0033] (1) Take fresh Pachyphyllum leaves, wash and dry them, put them into an extraction tank, add 10 times the amount of water, heat and reflux 3 times, filter, combine the extracts, concentrate, and obtain the water extract of Pachyphyllum parenchyma leaves for subsequent use;

[0034] (2) Add ethanol to the water extract of leaves of Pachyphyllum obtained in step (1) to make the concentration of ethanol in the water extract of leaves of Pachyphylla reach 65% to 75%, leave it to stand, precipitate, filter out the solution, and concentrate to obtain the water extract of leaves of Pachyphylla The alcohol precipitation solution of the liquid is ready for use;

[0035] (3) take the ethanol precipitation liquid obtained in step (2) and put it on the macroporous resin column, first use water and a concentration of 10% ethanol to elute, and then use a concentration of 50% to 55...

Embodiment 2

[0036] Embodiment 2 Acute toxicity test and maximum dosage determination

[0037] The test results of the maximum dosage of the total flavonoids from the leaves of the bamboo leaves prepared in Example 1 by intragastric administration showed that 20 mice had no toxic reaction after administration, no obvious changes in major tissues and organs, and no death within 7 days. The maximum dosage of the total flavonoids of Danzhu leaves administered to mice was 14.14g·kg -1 , which is equivalent to 176.8 times the maximum pharmacodynamic dose. It shows that the total flavonoids of Pachyphyllum flavonoids provided by the invention are isolated from natural Pachyphyllum pachyrhiza leaves, have low toxicity and good safety.

Embodiment 3

[0038] Embodiment 3 Anti-inflammatory experiment

[0039] Fifty female mice weighing (20±3) g were provided by the Animal Experiment Center of Nanjing University of Traditional Chinese Medicine.

[0040] They were randomly divided into five groups including blank control group, xylene group, and the total flavonoids of Pachyphyllum flavonoids prepared in Example 1, high, medium, and low, with 10 animals in each group. The animals in the treatment group were administered intragastrically continuously for 7 days, and the blank control group was given an equal volume of normal saline. One hour after the last administration, 25 μl of xylene was applied to the front and back sides of the right ear of each mouse. After 2 hours, the animals were sacrificed, and round ears were punched at the same part of both ears with an 8 mm diameter punch, and the balance was analyzed. weighing. The swelling degree was determined by subtracting the weight of the left ear piece from the weight of...

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Abstract

The invention discloses application of lophatherum gracile total flavonoids in preparation of medicine and functional food for preventing and treating myocardial ischemia disease. Shown by the experiment results, the lophatherum gracile total flavonoids are safe without toxic or side effect, and have obvious inhibiting effect on ear swelling model caused by p-dimethylbenzene, can inhibit platelet aggregation and prolong the thrombosis time, and have better protection effect on rat myocardial ischemia and myocardial ischemia reperfusion injury caused by pituitrin. Furthermore, shown by screening results of a great amount of experiments, after being combined with antiplatelet aggregation medication, beta-receptor blocking pharmacon, calcium ion antagonist, statins, RAS system blocking agent, nitrite medication and thrombolysis medication or angiotensin-converting enzyme inhibitor, the the lophatherum gracile total flavonoids show better antiplatelet aggregation, is capable of prolonging the thrombosis time, have better protection effect on rat myocardial ischemia and myocardial ischemia reperfusion injury caused by pituitrin, and play a better synergistic effect.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract, in particular to the application of the total flavonoids of Pachyphyllum flavonoids in the preparation of medicines and functional foods for preventing and treating myocardial ischemia. Background technique [0002] In recent years, with the improvement of living standards, myocardial ischemia seriously endangers the health of middle-aged and elderly people. Coronary atherosclerosis presents a younger trend, and some young people also appear myocardial ischemia. [0003] Cardiovascular disease, also known as circulatory system disease, refers to the organs and tissues that transport blood in the human body, mainly including the heart and blood vessels, which can be subdivided into acute and chronic diseases, and are generally related to arteriosclerosis. [0004] Myocardial ischemia refers to a pathological state in which the blood perfusion of the heart is reduced, resulting in reduced o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/899A61K45/00A61P9/10
Inventor 吴启南周婧乐巍邵莹陈广云王新胜
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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