Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Intraspecific gene improvement method for common tobacco mosaic virus resistance

A mosaic virus and tobacco technology, applied in the biological field, can solve the problems of difficulty in cultivating germplasm materials, long period of hybrid breeding, etc.

Inactive Publication Date: 2012-09-26
GUIZHOU TOBACCO SCI INST
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The breeding of TMV-resistant tobacco germplasm is mainly through traditional cross breeding, but it is difficult to effectively break the linkage between resistance genes and unfavorable trait genes. In addition, the long cycle of cross breeding makes it difficult to cultivate high-quality and usable germplasm materials. Therefore, tobacco Intraspecific gene transformation for TMV resistance combined with marker-free gene selection to improve tobacco will have broad application potential

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Intraspecific gene improvement method for common tobacco mosaic virus resistance
  • Intraspecific gene improvement method for common tobacco mosaic virus resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0039] A method for improving the resistance of tobacco common mosaic virus in-species, comprising the following steps:

[0040] (1) Clone Tobacco Ubi.u4 Constitutive expression of the gene promoter Pubi.u4:

[0041] Genomic DNA was extracted from the leaves of common tobacco variety K326. According to the characteristics of the Pubi.u4 promoter sequence provided in Genbank Accession (Genbank Accession): X77456, the amplification primers were designed and synthesized, and the upstream primer Ubi-1 was introduced Hind III enzyme cleavage site, introduction of downstream primer Ubi-2 Xba I restriction site. Tobacco cloned by PCR method Ubi.u4 Gene 796 bp of Pubi.u4 promoter.

[0042] The amplification primers for the Pubi.u4 promoter are:

[0043] Ubi-1: 5'-CCC AAG CTT GGA GGC TAA CTA CGT TAG AGC-3';

[0044] Ubi-2: 5'-TGC TCT AGA TCT GTA TAT ACA GAA AAG GTT-3';

[0045] (2) Synthetic terminator Tubi.u4 and LB-MCS-RB sequence:

[0046] According to the Tubi.u4 termina...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an intraspecific gene improvement method for common tobacco mosaic virus resistance, belonging to the technical field of biology. The method comprises the following steps of: (1) building a Pubi.u4 promoter from the common tobacco to drive the TMV-N (tobacco mosaic virus) gene expression and the tobacco intraspecific gene expression vector pSH-P-N-T of which expression is stopped by Tubi.u4; (2) carrying out co-transformation on the tobacco explant by the intraspecific gene expression vector pSH-P-N-T and the expression vector pSh737 which only contains the marker gene through sonication-assisted agrobacterium-mediated transformation, and screening to obtain T0 generation transformed tobacco plant; and (3) carrying out T1 generation genetic segregation and screening test to obtain the intraspecific gene improvement plant of the marker gene-free common tobacco mosaic virus resistance (TMW). According to the method, the maker-free transformation of the common tobacco can be realized, and the influence of the non-tobacco source gene sequence can be eliminated, so that the transgenosis safety can be furthest realized.

Description

Technical field [0001] The present invention is a biotechnology field. Specifically, it is a method that involves a way to improve the general tobacco species using gene expression regulatory sequences and functional genes in ordinary tobacco species. [0002] Background technique [0003] GM technology breaks the boundaries of the species, so that genes between different species can perform unprecedented new combinations. However, in genetically modified crop breeding, the functional genes, promoters, and terminate sub -expression of other species are often introduced.Β-glucosaconase (GUS) gene, green fluorescent protein (GFP) gene, antibiotics or herbicide resistance genes and other selection marks.While showing the excellent characteristics of these genetically modified crops, they also bring environmental safety and food safety such as environmental safety and food safety. In particular, genetically modified plants are used to produce functional foods, medicines and industria...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/84A01H5/00C12Q1/68C12Q1/34
Inventor 赵杰宏王轶韩洁赵丹
Owner GUIZHOU TOBACCO SCI INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com