Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing catalase by using bovine livers

A technology of catalase and bovine liver, which is applied in the direction of biochemical equipment and methods, enzymes, enzymes, etc., can solve the problems of no industrial production, and achieve the effects of high yield, short extraction time and simple operation

Inactive Publication Date: 2012-09-26
TIBET TIANCHENG AGRI ANIMAL HUSBANDRY IND
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of catalase by microbial fermentation in China is only in the laboratory research stage, and there is no industrial production, and the research on extracting catalase from animal raw materials has only attracted attention in recent years

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing catalase by using bovine livers

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] Weigh 5 kg of fresh beef liver, wash it with tap water, chop it up, mash it with a tissue mashing homogenizer, and make a homogenate for subsequent use. Take the bovine liver homogenate, use phosphate buffer (pH 7.2) as the extraction agent, the ratio of bovine liver and extraction agent is 1:3, the extraction temperature is 30°C, and the ultrasonic intensity is 30W. Hydrogenase extract. The above extract was passed through a DEAE-Sepharose ion-exchange chromatography column, equilibrated with phosphate buffer (0.05mol / L, pH 7.2) for 4 times the column volume, and loaded; pH 7.2 phosphate buffer) for gradient elution at a flow rate of 30 mL / h. The above-mentioned enzyme solution was subjected to gel filtration chromatography, using a Sephacryl S-200 gel column, washed with phosphate buffer (0.05mol / L, pH 7.2) after packing, loading, and washed with phosphate buffer (0.05mol / L , pH 7.2) for elution at a flow rate of 18 mL / h. Then, the enzyme solution was dialyzed at 4...

example 2

[0026] Weigh 10 kg of fresh beef liver, wash it with tap water, chop it up, mash it with a tissue mashing homogenizer, and make a homogenate for subsequent use. Take the bovine liver homogenate, use phosphate buffer (pH 7.2) as the extraction agent, the ratio of bovine liver and extraction agent is 1:4, the extraction temperature is 30°C, and the ultrasonic intensity is 40W and treated for 15 minutes to obtain peroxidized Hydrogenase extract. The above extract was passed through a DEAE-Sepharose ion-exchange chromatography column, equilibrated with phosphate buffer (0.05mol / L, pH 7.2) for 5 times the column volume, and loaded; with 0-1mol / L NaCl solution (containing 0.05mol / L, pH phosphate buffer in 7.2) for gradient elution at a flow rate of 30 mL / h. The above-mentioned enzyme solution was subjected to gel filtration chromatography, and a Sephacryl S-200 gel column was used. After loading the column, it was washed with phosphate buffer (0.05 mol / L, pH 7.2), and the sample wa...

example 3

[0028] Weigh 50 kg of fresh beef liver, wash it with tap water, chop it up, mash it with a tissue mashing homogenizer, and make a homogenate for subsequent use. Take the bovine liver homogenate, use phosphate buffer (pH 7.2) as the extraction agent, the ratio of bovine liver and extraction agent is 1:5, the extraction temperature is 30°C, and the ultrasonic intensity is 45W and treated for 20min to obtain peroxidized Hydrogenase extract. The above extract was passed through a DEAE-Sepharose ion-exchange chromatography column, equilibrated with phosphate buffer (0.05mol / L, pH 7.2) for 5 times the column volume, and loaded; with 0-1mol / L NaCl solution (containing 0.05mol / L, pH phosphate buffer in 7.2) for gradient elution at a flow rate of 35 mL / h. The above-mentioned enzyme solution was subjected to gel filtration chromatography, and a Sephacryl S-200 gel column was used. After loading the column, it was washed with phosphate buffer (0.05 mol / L, pH 7.2), and the sample was loa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for preparing catalase by using bovine livers, wherein fresh bovine livers are used as raw materials and subjected to chopping and homogenization, and a catalase product with high enzyme activity is obtained by means of processes such as ultrasonic extraction, ion exchange chromatography, gel filtration chromatography and freeze drying. The method of the invention solves the problems of complex production process, heavy pollution, high production cost and low product yield of the prior art in preparing the product. The catalase prepared by the method of the invention has an enzyme activity of 6000 U / mg and a colony count of less than or equal to 1000 cells / ml, and is suitable to be widely used in the fields of food, paper making and textile industry.

Description

technical field [0001] The invention relates to a preparation process of catalase. Specifically, the present invention uses fresh bovine liver as a raw material, chops it and homogenizes it, and adopts modern techniques such as ultrasonic extraction, ion exchange chromatography, gel filtration column chromatography and freeze-drying to prepare hydrogen peroxide with high enzymatic activity. An enzyme product belongs to the technical field of enzyme products. Background technique [0002] Catalase (CAT), also known as catalase, is a type of terminal oxidase that widely exists in animals, plants and microorganisms. It uses hydrogen peroxide as a substrate and catalyzes the transfer of a pair of electrons to eventually Break it down into water and oxygen. Catalase is a binding enzyme with iron porphyrin as the prosthetic group. One molecule contains 4 iron atoms. The active group is heme. The relative molecular weight is generally 200-340ku. It exists in almost all organisms....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08
Inventor 李少民
Owner TIBET TIANCHENG AGRI ANIMAL HUSBANDRY IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com