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Method for ultrasonic extraction and content determination for total gambogic acid in gamboge medicinal materials

A technology of extraction method and determination method, which is applied in the direction of medical formula, drug combination, antineoplastic drugs, etc., can solve the problems of not reflecting the total acid content of garcinia cambogia, unsatisfactory reproducibility, not easy to popularize, etc., and achieve high yield , Short extraction time, easy operation

Inactive Publication Date: 2012-09-26
彭代银
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"Chinese Pharmacist" 2002, 5 (12): 731-732 reported the use of zirconium oxychloride (ZrOCl2) colorimetric method to determine the total acid content of gamboge, but when using this method, the color reaction is easily affected by temperature and light and many other factors, resulting in unsatisfactory reproducibility (Pharmaceutical Advances, 2011, 35 (8): 337-344)
Thin-layer chromatography and high-performance liquid chromatography often can only quantify one or several index components when measuring gamboge total acid, and cannot reflect the content of gamboge total acid. Corresponding reference substances must be available, and the equipment is expensive. Not easy to popularize

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Ultrasonic Extraction of Gambogia Total Acid Components in Gamboge Medicinal Materials

[0043] Weigh 50g of 40-mesh gamboge dried medicinal material powder, load it in a 2000ml flask, add 250ml of 50% ethanol, and extract at 30°C for 10min in an ultrasonic wave with a frequency of 20 kHz. Filtrate, recover the solvent under reduced pressure to a paste (control the concentration temperature not to exceed 50°C), and dry under reduced pressure (control the concentration temperature to not exceed 50°C), to obtain 30.7g of the total acid fraction of Gamboge, the total acid content of Gamboge is 79.5% .

Embodiment 2

[0044] Example 2 Ultrasonic Extraction of Gambogia Total Acid Components in Gamboge Medicinal Materials

[0045] Weigh 50g of 40-mesh gamboge dried medicinal material powder, load it into a 2000ml flask, add 500ml of 70% ethanol, extract in an ultrasonic wave with a frequency of 30 kHz at 40°C for 15min, and after the extraction is completed twice, combine the filtrate and filter the filtrate , recover the solvent under reduced pressure to a paste (control the concentration temperature not to exceed 50°C), and dry under reduced pressure (control the concentration temperature to not exceed 50°C), to obtain 33.9g of the total acid fraction of Gamboge, and the total acid content of Gamboge is 81.1%.

Embodiment 3

[0046] Example 3 Ultrasonic Extraction of Gambogia Total Acid Components in Gamboge Medicinal Materials

[0047] Weigh 50g of 40-mesh gamboge dried medicinal material powder, load it in a 2000ml flask, add 750ml of 90% ethanol, and extract it at 50°C for 15min in an ultrasonic wave with a frequency of 50 kHz. Filtrate, recover the solvent under reduced pressure to a paste (control the concentration temperature not to exceed 50°C), and dry under reduced pressure (control the concentration temperature to not exceed 50°C), to obtain 36.5g of the total acid fraction of Gamboge, the total acid content of Gamboge is 82.9% .

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Abstract

The invention provides a method for ultrasonic extraction and content determination for total gambogic acid in gamboge medicinal materials, comprising the following steps of: drying the gamboge medicinal materials, crushing and then passing through a 40-mesh sieve, weighing crude powders, mixing with 50-100% ethanol solution in a solution-material ratio of 5:(1-20):1, extracting for 5-20 minutes at a temperature of 20-60 DEG C and an extraction frequency of 20-70 kHz to obtain a total gambogic acid extract, filtering, decompressing the filtrate to recover a solvent, concentrating into an extractum shape, and drying under a reduced pressure to obtain a total gambogic acid powder; and detecting the content of total gambogic acid in the gamboge medicinal materials and the extractive by ultraviolet spectrophotometry. The method provided by the invention is simple in extraction process flow, easy to control operation, and effectively shortens in extraction time; and the ultraviolet spectrophotometry is good in detection repeatability, simple in operation, high in accuracy, and capable of being used for quality control for the gamboge medicinal materials or the extractive.

Description

technical field [0001] The invention relates to the extraction and content detection of medicinal substances in traditional Chinese medicine, in particular to a method for ultrasonically assisted extraction of gamboge total acid from gamboge, and a content determination method for the gamboge total acid. Background technique [0002] The dry resin secreted by Garcinia hanburyiHook·f· of Garcinia genus (Gittiferae) plants, in the form of cylindrical or irregular lumps, yellow-brown, brittle and brittle. According to ancient Chinese medical records, gamboge tastes sour and astringent, poisonous, and has the effects of attacking toxins, eroding sores, breaking blood and dispelling stagnation, etc. It is mainly used to treat carbuncles, swollen toxins, stubborn ringworm, and malignant sores. The acidic component is the main active component in gamboge resin. The Chinese medicine Garcinia cambogia extract has significant inhibitory effects on mouse ascites liver cancer, Ehrlich a...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P35/00G01N21/33
Inventor 彭代银周安王效山李庆林李翔胡海霞
Owner 彭代银
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