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Application of lipase SMG1 in preparation of fatty acid monoglyceride

The technology of monoglyceride and lipase is applied in the application field of lipase SMG1 in the preparation of monoglyceride, which can solve the problems affecting product separation and purity, and achieve the effects of easy separation, easy realization and mild conditions.

Inactive Publication Date: 2012-09-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most lipases catalyze the synthesis of unsaturated fatty acid monoglycerides, which are accompanied by the production of a large number of diglycerides and triglycerides, which affect the separation and purity of the product.

Method used

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  • Application of lipase SMG1 in preparation of fatty acid monoglyceride
  • Application of lipase SMG1 in preparation of fatty acid monoglyceride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Put conjugated linoleic acid (CLA) and glycerol with a molar ratio of 1:5, and 1% water based on the total mass of the reaction substrate, into a stoppered Erlenmeyer flask, mix evenly, and place a magnetic stirrer at a speed of 400rpm For the reaction on a container, 320 U / g of lipase SMG1 based on the total mass of the reaction substrate was added, and the reaction was controlled at 5° C. by an incubator for 24 hours. After the reaction is finished, the reaction product is subjected to molecular distillation to separate and remove the fatty acid and diglyceride therein to obtain a product with monoglyceride as the main component. The reaction product composition, MAG purity and recovery rate after molecular distillation are shown in Table 1 and Table 2.

Embodiment 2

[0038] The amount of enzyme added is 500U / g, and all the other operating conditions are the same as in Example 1. The reaction product composition, MAG purity and recovery rate after molecular distillation are shown in Table 1 and Table 2.

Embodiment 3

[0040] Conjugated linoleic acid (CLA) and glycerin with a molar ratio of 1:3 were used, and the amount of enzyme added was 50 U / g, and the rest of the operating conditions were the same as in Example 1. The reaction product composition, MAG purity and recovery rate after molecular distillation are shown in Table 1 and Table 2.

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Abstract

The invention relates to application of lipase SMG1 in preparation of fatty acid monoglyceride, which comprises the following steps of: (1) preparing glycerol and fatty acid donor, adopting partial glyceride hydrolase SMG1 from Malasseziaglobosa as catalyst, and adding 1%-5% of water to catalyze reaction of the glycerol and fatty acid donor at 5-15 DEG C; and (2) separating, and removing lipase and glycerol from the product obtained from the step (1), separating and removing diglyceride and fatty acid to obtain the fatty acid monoglyceride. The glycerol and fatty acid donor can be used as a raw material in the invention, and esterification of the glycerol and fatty acid donor is catalyzed through lipase SMG1 at low temperature so as to generate the fatty acid monoglyceride. The prepared product has few by-products, light color and high yield.

Description

technical field [0001] The invention relates to the application of lipase SMG1 in the preparation of monoglycerides. Background technique [0002] Monoglycerides of fatty acids (abbreviated as monoglycerides), the English name monoglyceride, is an important class of nonionic surfactants. One lipophilic long-chain alkyl group and two hydrophilic hydroxyl groups make monoglycerides have a good surface active. Monoglycerides are widely used in the food and dairy industries as emulsifiers, in household chemicals such as cosmetics, toothpaste and hair conditioners as tissue modifiers, and in bread making as antifreeze agents. In recent years, it has been found that monounsaturated fatty acid glycerides are expected to enter the ranks of chemical preservatives. In addition, monoglycerides also have important applications in industries such as medicine, cosmetics, plastics, textiles, and polymer processing. For example, monoglycerides containing polyunsaturated fatty acids (CLA, ...

Claims

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Application Information

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IPC IPC(8): C12P7/62C12N9/20C12N15/55
Inventor 王永华徐达杨博孙丽娟侯淑琳陈华勇
Owner SOUTH CHINA UNIV OF TECH
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