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Pseudomonas putida strain for bio-control of Tobacco mosaic virus

A technology of Pseudomonas putida and mosaic virus, which can be used in bacteria, biocides, microorganisms, etc.

Active Publication Date: 2013-10-02
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] There have been reports on the biological control of common tobacco mosaic virus disease in China, but most of them are limited to the screening of bio-control bacteria and pot experiments, and the extraction of antibacterial substances, physiological and biochemical characteristics and disease prevention mechanism of bio-control bacteria, etc. Many aspects have not been studied in depth in China

Method used

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  • Pseudomonas putida strain for bio-control of Tobacco mosaic virus
  • Pseudomonas putida strain for bio-control of Tobacco mosaic virus
  • Pseudomonas putida strain for bio-control of Tobacco mosaic virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0052] Observation of the effect of A3 strain active substances on TMV virions under fluoroscopic electron microscope

[0053] After the purified TMV was treated by negative staining, observed by JEM-100CX transmission electron microscope, the TMV granules in the field of view were typical rod-shaped, about 500 nm in length, straight and neatly arranged, with good integrity; After 2 min of mixed treatment, the electron microscope observation showed that the TMV mitochondrion in the visual field changed significantly and almost all broke into small rods, and the arrangement was disordered; The results of liquid treatment of TMV mitochondria were the same, and TMV mitochondria were also broken into small fragments and arranged disorderly.

[0054] It can be seen that the A3 strain fermentation broth and the active extract in the strain have a strong destruction effect on TMV granules, and the destruction methods are the same. It can be preliminarily speculated that the antagonis...

Embodiment 3

[0058] The A3 strain was inoculated into about 3L of NB liquid medium, 28°C, 180rpm, and cultivated for 60h-72h to obtain the A3 strain fermentation broth. Tween-20 was added to the fermentation broth as a leaf surface extender, and the concentration of Tween-20 was respectively prepared. 500 mL of each concentration was prepared for 3%, 2%, 1%, 0.5% and 0.25% agents.

[0059] The medicament adopts the foliar spray method. When the NC89 tobacco seedlings grow to the 4-5 leaf stage, the medicament of the corresponding concentration is evenly sprayed on the leaves with a sterile sprayer, and 12 plants are sprayed at each concentration, and then the 1d, 1d, After 2d, 3d, and 4d, inoculate TMV juice (3 plants per day), and use cotton swabs to keep the inoculation intensity as consistent as possible. Tween at the corresponding concentration was added to the NB liquid medium as a negative control for each concentration; the antiviral agent-Ningnamycin and soapy water diluted to 3%, ...

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Abstract

The invention discloses a Pseudomonas putida strain for bio-control of Tobacco mosaic virus, wherein the strain code is A3; the strain is preserved in the General microbial center of China Committee for Culture Collection of Microorganisms on 7 September 2011, and the preservation number of the strain is CGMCC No.5231. The strain can restrain invasion of the Tobacco mosaic virus. The bio-control experiment shows that the Tobacco mosaic virus can be prevented and treated. Simultaneously, the invention also provides a mixture of active small molecule substances obtained from the strain, and the mixture has high application value in the respect of prevention and treatment of the Tobacco mosaic virus.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a Tobacco common mosaic virus biocontrol Pseudomonas putida strain, and the application of the strain and a mixture of active small molecular substances obtained from the strain in the prevention and control of Tobacco Common Mosaic Virus. Background technique [0002] Tobacco virus disease is one of the main diseases in tobacco areas in the world, which causes huge economic losses every year. my country's tobacco virus diseases have been documented in the early days of the founding of the People's Republic of China, and since the 1970s, the harm of virus diseases has increased year by year. From 1989 to 1991, the National Tobacco Monopoly Administration-funded scientific research project "National Tobacco Infectious Disease Investigation and Research" identified 16 viruses that cause tobacco virus diseases in my country, of which 4 are widely distributed and seriously harmful: includin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00C12P1/04C12R1/40
Inventor 王凤龙孙惠青钱玉梅杨金广郭丛陈德鑫申莉莉王新伟战徊旭王秀芳陈丹
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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