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Pseudomonas putida strain for bio-control of Tobacco mosaic virus

A technology of Pseudomonas putida and mosaic virus, applied in the direction of bacteria, biocides, microorganisms, etc., can solve the problems that the mechanism of disease prevention of physiological and biochemical characteristics has not been studied in depth

Active Publication Date: 2012-09-19
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Description
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AI Technical Summary

Problems solved by technology

[0014] There have been reports on the biological control of common tobacco mosaic virus disease in China, but most of them are limited to the screening of bio-control bacteria and pot experiments, and the extraction of antibacterial substances, physiological and biochemical characteristics and disease prevention mechanism of bio-control bacteria, etc. Many aspects have not been studied in depth in China

Method used

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  • Pseudomonas putida strain for bio-control of Tobacco mosaic virus
  • Pseudomonas putida strain for bio-control of Tobacco mosaic virus
  • Pseudomonas putida strain for bio-control of Tobacco mosaic virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0052] Observation of Effect of Active Substances of A3 Strain on TMV Virus Particles under a Perspective Electron Microscope

[0053] After the purified TMV was treated by negative staining, the TMV granules in the field of view were typical rod-shaped, about 500nm in length, straight and neatly arranged, and well-integrated under the JEM-100CX transmission electron microscope; After 2 minutes of mixed treatment, the electron microscope observation showed that the TMV granules in the field of vision had significant changes, and almost all of them were broken into small rods, and the arrangement was messy and disorderly. The result of liquid treatment of TMV granules was the same. TMV granules were also broken into small fragments and arranged disorderly.

[0054] It can be seen that both the fermentation broth of the A3 strain and the active extract in the strain have a strong destructive effect on TMV mitochondria, and the destruction methods are the same. It can be prelimin...

Embodiment 3

[0058] Inoculate A3 strain into about 3L of NB liquid medium, 28°C, 180rpm, culture for 60h-72h, obtain A3 strain fermentation broth, add Tween-20 into the fermentation broth as a foliage extender, and prepare Tween-20 concentrations respectively Prepare 500 mL of each concentration for 3%, 2%, 1%, 0.5% and 0.25% agent.

[0059] The medicament adopts the method of foliar spraying. When the NC89 tobacco seedlings grow to the 4-5 leaf stage, use a sterile sprayer to evenly spray the corresponding concentration of the medicament on the leaves. After 2d, 3d, and 4d, inoculate TMV juice (inoculate 3 strains per day), and use cotton swabs to keep the inoculation intensity as consistent as possible. NB liquid culture medium was added Tween of corresponding concentration as the negative control of each concentration; antiviral agent-Ningnanmycin, soapy water diluted to 3%, 2%, 1%, 0.5%, and 0.25% concentrations were respectively regarded as positive control. The observation results ...

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Abstract

The invention discloses a Pseudomonas putida strain for bio-control of Tobacco mosaic virus, wherein the strain code is A3; the strain is preserved in the General microbial center of China Committee for Culture Collection of Microorganisms on 7 September 2011, and the preservation number of the strain is CGMCC No.5231. The strain can restrain invasion of the Tobacco mosaic virus. The bio-control experiment shows that the Tobacco mosaic virus can be prevented and treated. Simultaneously, the invention also provides a mixture of active small molecule substances obtained from the strain, and themixture has high application value in the respect of prevention and treatment of the Tobacco mosaic virus.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a strain of Pseudomonas putida for the biocontrol of tobacco common mosaic virus, and the application of the strain and a mixture of active small molecular substances obtained from the strain in the prevention and control of tobacco common mosaic virus. Background technique [0002] Tobacco viral diseases are one of the main diseases in various smoking areas in the world, causing huge economic losses every year. Tobacco virus diseases in China have been documented in the early days of the founding of the People's Republic of my country. After entering the 1970s, the harm of tobacco virus diseases has increased year by year. From 1989 to 1991, the State Tobacco Monopoly Administration funded the scientific research project "National Tobacco Infectious Disease Investigation and Research", and found out that there are 16 kinds of viruses that cause tobacco viral diseases in my country, of...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00C12P1/04C12R1/40
Inventor 王凤龙孙惠青钱玉梅杨金广郭丛陈德鑫申莉莉王新伟战徊旭王秀芳陈丹
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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