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Modified model for evaluating iron biological effectiveness in foods and establishment method thereof

A technology for bioavailability and establishment of methods, applied in the field of improved models for evaluating dietary iron bioavailability and its establishment, can solve problems such as iron bioavailability limitations, and achieve the effect of simulating transport and metabolic characteristics

Inactive Publication Date: 2012-09-19
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of human experiments to evaluate the bioavailability of dietary iron is limited due to concerns about ethics and human life safety

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Evaluation method of iron bioavailability in commercial iron enhancer A

[0022] (1) Caco-2 cell culture

[0023] Put the insertion slot into the six-well culture plate; set the cell density to 3×10 5 1.5mL of the Caco-2 cell mixture of serotonin was dripped onto the bottom membrane of the insertion slot, and at the same time, 2mL of DMEM containing 10% fetal bovine serum was added to the basal side;

[0024] (2) 293T cell culture

[0025] Culture human kidney embryo T (293T) cells on the basal side of another six-well plate with the same culture medium as Caco-2 cells;

[0026] (3) Model establishment

[0027] Move the insertion slot where Caco-2 cells are cultured into a six-well plate where 293T cells are cultured, and co-culture for 24 hours;

[0028] (4) Iron bioavailability test

[0029] Grind the commercially available iron enhancer A, use the traditional digestive system to simulate digestion, centrifuge to obtain the supernatant, mix the supernatant with the HBSS...

Embodiment 2

[0033] Example 2: Evaluation method of the bioavailability of rice 1 and rice 2 to human iron

[0034] (1) Caco-2 cell culture

[0035] Put the insertion slot into the six-well culture plate; set the cell density to 3×10 5 1.5mL of the Caco-2 cell mixture of serotonin was dripped onto the bottom membrane of the insertion slot, and at the same time, 2mL of DMEM containing 10% fetal bovine serum was added to the basal side;

[0036] (2) 293T cell culture

[0037] Culture human kidney embryo T (293T) cells on the basal side of another six-well plate with the same culture medium as Caco-2 cells;

[0038] (3) Model establishment

[0039] Move the insertion slot where Caco-2 cells are cultured into a six-well plate where 293T cells are cultured, and co-culture for 25 hours;

[0040] (4) Iron bioavailability test

[0041] The rice 1 and rice 2 are steamed, mashed, simulated digested with a traditional digestive system, centrifuged, and the supernatant is obtained. The supernatant is mixed with th...

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PUM

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Abstract

The invention relates to a modified model for evaluating iron biological effectiveness in foods and an establishment method thereof. The model comprises a model structure, cell types and evaluation indexes; an insertion slot is formed in the model structure, and a Caco-2 cell culture position is arranged on a basement membrane of the insertion slot; and the model structure is provided with a top side chamber and a substrate side chamber. The iron absorption and transportation model is similar to a human intestinal absorption structure on the model structure, can simulate the iron absorbing process of a human body and iron transportation and metabolism characteristics of the human body, and overcomes a defect that the conventional model is limited to iron concentration of a sample to be detected.

Description

Technical field [0001] The invention relates to a model suitable for evaluating the bioavailability of iron in cereal grains, iron fortifiers and animal foods, and also relates to a method for establishing the model. Background technique [0002] Iron deficiency is a world-wide micronutrient hunger problem. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO), more than 3.5 billion people in the world lack iron, especially adolescents, pregnant women, the elderly, and infants. , Iron deficiency anemia reaches 1.48 billion. Iron deficiency has severely affected the physical health, intellectual development and work ability of people all over the world. Eliminating human iron nutrient hunger has become one of the urgent problems that countries all over the world need to overcome. Dietary diversity, food iron fortification, and iron supplementation are important ways to solve human iron deficiency....

Claims

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Application Information

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IPC IPC(8): C12M1/00C12Q1/02G01N33/68G01N27/62G01N27/626
Inventor 何万领李晓丽王占彬李元晓张才李旺曹平华
Owner HENAN UNIV OF SCI & TECH
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