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Method for preparing membrane materials through biomolecular self assembly under the condition of cold plasma

A technology of cold plasma and biomolecules, which is applied in the fields of chemistry, ion physics, biological science and material science, can solve the problems of complex operation, difficult control, cumbersome process, etc., and achieve uniform thickness, easy operation and less time-consuming Effect

Inactive Publication Date: 2012-09-19
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It can be seen that these methods are cumbersome in process, complicated in operation, harsh in conditions and difficult to control, and use organic solvents, some of which are highly toxic

Method used

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  • Method for preparing membrane materials through biomolecular self assembly under the condition of cold plasma
  • Method for preparing membrane materials through biomolecular self assembly under the condition of cold plasma
  • Method for preparing membrane materials through biomolecular self assembly under the condition of cold plasma

Examples

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Effect test

Embodiment 1

[0026] Dissolve the polypeptide molecules in three-distilled water to prepare solutions with concentrations of 20 μmol / L, 50 μmol / L, 100 μmol / L, 150 μmol / L and 200 μmol / L, and place the solutions between the two electrode plates of the discharge tube in the vacuum chamber , sealed, the vacuum chamber is evacuated, and then filled with argon or helium as a discharge gas, a DC voltage is applied to the electrode, and a cold plasma is used for treatment. In order to examine the effect of the pressure in the vacuum chamber on the results, we used 50Pa, 100Pa, 150Pa and 200Pa pressures for each concentration of the solution. In order to examine the effect of applying different DC voltages on the results, we applied five different voltages to each concentration solution: 100V, 500V, 1000V, 2000V and 3000V. In order to investigate the effect of plasma treatment time on the results, we performed plasma treatment for 1min, 3min, 5min, 8min, 10min, 12min and 15min on the solutions of ea...

Embodiment 2

[0036] Dissolve protein molecules in triple distilled water to make solutions with concentrations of 20μmol / L, 50μmol / L, 100μmol / L, 150μmol / L and 200μmol / L, and place the solution between the two electrode plates of the discharge tube in the vacuum chamber , sealed, the vacuum chamber is evacuated, and then filled with argon or helium as a discharge gas, a DC voltage is applied to the electrode, and a cold plasma is used for treatment. In order to examine the effect of the pressure in the vacuum chamber on the results, we used 50Pa, 100Pa, 150Pa and 200Pa pressures for each concentration of the solution. In order to examine the effect of applying different DC voltages on the results, we applied five different voltages to each concentration solution: 100V, 500V, 1000V, 2000V and 3000V. In order to investigate the effect of plasma treatment time on the results, we performed plasma treatment for 1min, 3min, 5min, 8min, 10min, 12min and 15min on the solutions of each concentration...

Embodiment 3

[0045] Dissolve trypsin molecules in three-distilled water to make solutions with concentrations of 20μmol / L, 50μmol / L, 100μmol / L, 150μmol / L and 200μmol / L, and place the solution between the two electrode plates of the discharge tube in the vacuum chamber The room is sealed, and the vacuum chamber is evacuated, and then filled with argon or helium as a discharge gas, and a DC voltage is applied to the electrodes, and treated with cold plasma. In order to examine the effect of the pressure in the vacuum chamber on the results, we used 50Pa, 100Pa, 150Pa and 200Pa pressures for each concentration of the solution. In order to examine the effect of applying different DC voltages on the results, we applied five different voltages to each concentration solution: 100V, 500V, 1000V, 2000V and 3000V. In order to investigate the effect of plasma treatment time on the results, we performed plasma treatment for 1min, 3min, 5min, 8min, 10min, 12min and 15min on the solutions of each concen...

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Abstract

The invention relates to a method for preparing membrane materials through biomolecular self assembly under the condition of cold plasma, which comprises the following steps of: dissolving biomolecules in triple distilled water to prepare a solution with the concentration of 20-200 mol / L; directly putting the biomolecular solution into a plasma discharger; vacuumizing the plasma discharger, and then leading plasma discharge gas with the pressure of 50-200 Pa; utilizing a high-voltage power supply to apply 100-3000 V direct current or alternating current to both ends of an electrode to make the discharge gas discharge and form plasma with the processing time of 1-15 minutes; and putting the solution into a shaking table at a constant temperature to be cultured for 5-240 hours after the plasma is processed. According to the method, cold plasma is integrated into the process of the biomolecular self assembly, and products of the biomolecular self assembly are changed from nanofibers, nanorods, nanotubes or nanoparticles into biological membranes. The method has the advantages of easiness and convenience for operation, less time consumption, high efficiency, low energy consumption and friendliness to environment.

Description

technical field [0001] The invention relates to the technical fields of plasma physics, chemistry, biological science, material science and the like. In particular, it is a method for preparing membrane materials by self-assembly of biomolecules under cold plasma conditions. Background technique [0002] Molecular self-assembly is the most common phenomenon in nature, especially in life systems, and one of the most essential contents of life sciences. The formation of polypeptides, DNA, proteins, cells and even life is all achieved through self-assembly. The so-called molecular self-assembly refers to the arrangement and assembly of molecules into nano- or micro-scale ordered structures through short-range forces, such as van der Waals forces, hydrogen bonds, electrostatic interactions, and hydrophobic interactions. Carrying out research on molecular self-assembly is of great significance. It helps people understand the formation and evolution of life in nature at the molec...

Claims

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Application Information

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IPC IPC(8): B05D7/24B82Y5/00B82Y40/00
Inventor 刘昌俊潘云翔于越
Owner TIANJIN UNIV
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