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Moringa extract as well as preparation method of moringa extract and moringa feed additive

A technology of moringa extract and moringa leaf, applied in animal feed, animal feed, application, etc., can solve the problems of low yield, low content, stimulating animal growth, etc., and achieve the effect of high quality and high yield

Inactive Publication Date: 2014-07-09
HAINAN STAND BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the general method of adding Moringa leaves to feed is to crush them into powder and mix them into the feed, but this method is relatively rough, not only the animal absorbs slowly but also consumes a lot of Moringa leaves, and the active ingredients are not clear, so it cannot be effective. Improves feed utilization and stimulates animal growth
There are reports of water extraction and alcohol extraction of Moringa leaves in the prior art, and these two technologies are used to make Moringa extracts and add them to feed, so that the active ingredients of Moringa leaves are directly utilized by animals. The yield of these two kinds of methods is lower, and in the extracted Moringa oleifera extract, the active ingredients beneficial to animal growth, such as the content of substances such as protein, flavonoids, polysaccharides and polyphenols are not high, which is helpful for improving feed utilization and Stimulation of animal growth still has some degree of limitation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: prepare Moringa oleifera extract with preparation method of the present invention

[0024] Wash, dry, and crush the leaves of Moringa oleifera into powder, put them into an extraction tank, add water 8 times the mass of leaves of Moringa oleifera, then adjust the pH to 5.5-6.3, and the temperature to 55-60°C, add papain (papaya The mass ratio of protease and Moringa leaves is 1:200) enzymatic hydrolysis for 2 hours, then adjust the pH value to 4.5-5.0, and the temperature is about 50°C, add cellulase and pectinase (the mass of cellulase and Moringa leaves The ratio is 1:125, the mass ratio of pectinase and Moringa leaf is 1:200) enzymolysis for 2h;

[0025] After the enzymolysis is completed, heat and boil for 1 hour, filter to obtain the first filtrate and filter residue, add 6 times the mass of water to the filter residue, continue heating and boiling for 1 hour, filter to obtain the second filtrate, and combine the two filtrates to obtain the water extr...

Embodiment 2

[0027] Embodiment 2: Yield and active substance content comparative test

[0028] Get Moringa oleifera leaves with the same source and equal quality as in Example 1 to prepare the Moringa oleifera extract by water extraction and alcohol extraction respectively, the preparation environment and conditions are consistent with Example 1, and the final statistics of the Moringa oleifera extract of the three methods The yield and the content of protein, flavonoids, polysaccharides and polyphenols are shown in Table 1.

[0029] Table 1 Active substance content test results

[0030]

[0031] As can be seen from the above table, no matter aspect the yield or aspect the content of active substances that are beneficial to animal growth, the Moringa oleifera extract prepared by the present invention is higher than the Moringa oleifera extract prepared by the other two methods, showing that the present invention The preparation method can increase the yield of Moringa oleifera e...

Embodiment 3

[0032] Embodiment 3: piglet growth test

[0033] Add three kinds of Moringa oleifera extracts in Example 2 respectively in common feed (outside common feed and Moringa oleifera extract, do not contain other substances), the addition amount is 2‰ of common feed quality, to 28 days old piglets Carry out feeding for 40 days, set up control group, observe and count the feeding results, see Table 2 for the addition.

[0034] Table 2 Piglet growth test results

[0035]

[0036] As can be seen from the results in the above table, using the feed added with Moringa extract of the present invention to feed piglets, the feed consumed by each pig every day is the least, but the daily gain is the highest, so the feed-to-meat ratio and cost It was also the least in several other groups, indicating that the present invention can improve feed utilization and stimulate animal growth.

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PUM

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Abstract

The invention relates to the technical field of biology, and discloses a moringa extract as well as a preparation method of the moringa extract and a moringa feed additive. According to the preparation method, water is added into moringa leaves, the pH value and the temperature are reduced, then, biological enzymes are adopted for enzymolysis, next, heating digestion is adopted to obtain water extraction liquid, ethanol precipitation is carried out after water extraction liquid concentration, obtained precipitates are the moringa extract, the biological enzymes are protease, cellulose and pectinase, and the protease is papain, pepsase, trypsin or serrapeptass. The enzymolysis effects of the specific protease, cellulose and pectinase is utilized for hydrolyzing large blocks of complicated-molecular-structure ingredients of the moringa leaves per se into smaller water-soluble high-bioavailability small molecular active substances, and then, high-yield and high-quality moringa extracts are obtained through a water extraction and ethanol precipitation method.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a moringa extract, a preparation method thereof and a moringa feed additive. Background technique [0002] Moringa, the Moringa family of the cauliflower order, native to India, also known as the drumstick tree, is a perennial tropical deciduous tree. The whole plant of Moringa oleifera can be used, and there are many and complete nutrients, especially the leaves of Moringa oleifera are the most widely used. Moringa leaves are pungent, slightly warm, and non-toxic. Its ingredients are rich in nutrients, including flavonoids, polyphenols, polysaccharides, proteins, vitamins and minerals, and essential amino acids that cannot be synthesized by the human body. Relevant research results show that as long as three tablespoons of Moringa leaf powder, it contains 27% of vitamin A, 42% of protein, 125% of calcium, 70% of iron, and 22% of vitamin C that children need daily. In terms of effi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/14A23K1/165
Inventor 韩金光王海生伍增利
Owner HAINAN STAND BIO TECH
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