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Siderophilic endophyte with antagonism on banana fusarium wilt germs and application of siderophilic endophyte

A banana fusarium wilt and banana technology, applied in the direction of application, chemicals for biological control, bacteria, etc., can solve the problems of large manpower and funds, high cost, long breeding cycle, etc., and achieve good antagonism and good growth promotion The effect of action

Inactive Publication Date: 2013-06-05
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although agricultural measures such as crop rotation are effective, they are costly and time-consuming, making it difficult to popularize them on a large scale
Chemical pesticides have problems such as poor control effect, polluting the environment, inhibiting beneficial microorganisms and inducing drug resistance of pathogens
The use of disease-resistant varieties has a long breeding cycle, requires a lot of manpower and funds, and is greatly restricted in production.

Method used

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  • Siderophilic endophyte with antagonism on banana fusarium wilt germs and application of siderophilic endophyte
  • Siderophilic endophyte with antagonism on banana fusarium wilt germs and application of siderophilic endophyte
  • Siderophilic endophyte with antagonism on banana fusarium wilt germs and application of siderophilic endophyte

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, screening and identification of antagonistic bacteria of Fusarium wilt of banana

[0023] 1. Screening of antagonistic bacteria against Fusarium wilt of banana

[0024] This strain was isolated from healthy Brazilian banana (Musa AAA, CV. BAXI) plants in the Banana Germplasm Garden of the Institute of Tropical Crops Variety Resources, Chinese Academy of Tropical Agricultural Sciences. The specific method is: weigh healthy banana plants (planted for 8 months) 1g of tissue, after surface disinfection, put it into a sterilized mortar with 9mL of sterile water, add a little sterilized quartz sand to grind evenly, and let it stand for 15min. Take 0.1mL and dilute to 10 -3 、10 -4 、10 -5 Concentration gradient, take 0.1mL from each concentration gradient suspension, spread it on NA medium (beef extract 3g, bacteriological peptone 10g, NaCl 5g, agar 15g, distilled water 1000mL, pH 7.0-7.2) plate with a sterile applicator Above, use the sterile water that washe...

Embodiment 2

[0033] Embodiment 2, the determination of the endogenous property of Ralstonia sp. BEB99 CGMCC NO.6024

[0034] 1. Acquisition of rifampin resistance in Ralstonia sp. BEB99 CGMCC NO.6024

[0035] Inoculate Ralstonia sp. BEB99 into the NB culture solution containing 5 μg / mL rifampicin, shake the bacteria until the bacteria solution becomes cloudy, take a small amount of cultured bacteria solution and inoculate it into the NB culture solution containing 10 μg / mL rifampicin. In Fuping's NB culture medium, shake the bacteria until the bacteria become cloudy. According to this method, gradually increase the concentration of rifampicin for cultivation, and finally obtain the NB culture solution containing 400 μg / mL rifampicin (beef extract 3g, bacteriological peptone 10g, NaCl 5g, distilled water 1000mL, pH 7.0-7.2) Ralstonia sp. (Ralstonia sp.) BEB99 thallus of normal growth in the medium, confirmed the biological characteristic of this thalline and antibacterial activity and Rals...

Embodiment 3

[0038] Example 3, detection of siderophores produced by Ralstonia sp. BEB99 CGMCC NO.6024

[0039] Some microorganisms are able to produce a special kind of organic compound that can bind iron, called siderophore. Its main group is a derivative of hydroxamic acid or catechol, which can firmly bind to ferric iron, dissolve ferric iron and transport iron into cells.

[0040] Detect the siderophore ability of Ralstonia sp. BEB99 CGMCC NO.6024 of the present invention by following experiment:

[0041] 1. Preparation of siderophore medium plates (containers and utensils used in the configuration process must be soaked in 6mol / L HCl solution and rinsed with double distilled water):

[0042] 1) First prepare 10×MM9 medium (formula: 3g of potassium dihydrogen phosphate, 5g of sodium chloride, 10g of ammonium chloride, 1000mL of double distilled water), and autoclave at 121°C for 20min.

[0043] 2) Add 6mL of 10mmol / L hexadecyltrimethylammonium bromide (HDTMA) solution into a 100mL v...

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Abstract

The invention discloses an antagonist for plant pathogenic fungi and application of the antagonist to prevention and treatment of plant diseases. The antagonist for the plant pathogenic fungi is Ralstonia sp. BEB99 CGMCC NO.6024. The antagonist Ralstonia sp. BEB99 can prevent and treat plant diseases caused by banana fusarium wilt germs, particularly, can be symbiotic with the banana, stably lives in the banana, can obviously promote growth of the banana plants, and can prevent and treat banana fusarium wilt.

Description

technical field [0001] The invention relates to a siderophilic endophyte with antagonistic effect on banana wilt and its application, in particular to a siderophilic endophytic with antagonistic effect on banana wilt and its application in preventing and treating banana wilt. Background technique [0002] Plant endophytic bacteria refer to bacteria that can enter living plant tissues during their lifetime or at least during a certain period of their lifetime, and their existence does not significantly change the phenotypic characteristics and functions of plant tissues. Unlike pathogenic bacteria, there is a symbiotic relationship between endophytic bacteria and host plants. In 1926, Perotti discovered endophytic bacteria in the root tissues of many healthy plants, and then the research in this area has made great progress. At present, it has been found that there are a large number of endophytic bacteria in almost all healthy plants. These endophytic bacteria can play some...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/01
Inventor 黄贵修戴英葵刘先宝时涛蔡吉苗林春花李超萍
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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