Preparation method for microarray biochip

Abstract

The invention relates to a preparation method for a microarray biochip. M print spray heads are used to constitute a spray head group, the spray heads are installed in the direction parallel to the X direction, and are arranged along the Y direction; a biochip substrate and the spray head group move step by step relatively along the Y direction, the action of a corresponding nozzle on the spray head corresponding to each step position is controlled by a driving signal, and a biomolecule solution of one index is sprayed onto the preset position of one or a plurality of microarray biochip substrates arranged along the X direction; and through the sequential action of the M spray heads, the biomolecule solutions of M indexes are sequentially sprayed onto the preset position of the biochip substrate to form a sample application array. The preparation for the microarray biochip used for multi-index and multi-user parallel detection can be realized. Compared with the existing product, the biochip prepared in the method disclosed by the invention has the characteristics of high speed, high precision, wide applicability, high efficiency, small cross contamination, easiness in maintenance and use, low manufacturing cost and the like, and the method is very suitable for the large-scale production of microarray biochips and is also suitable for scientific research and application.

Description

technical field [0001] The invention relates to a biochip preparation technology, in particular to a preparation method of a microarray biochip for microarray parallel detection. Background technique [0002] The preparation method of existing biochip can basically be divided into two classes: a class is in-situ synthesis (that is, the direct in-situ synthesis of oligonucleotide probes on the surface of the support); a class is directly sampled after pre-synthesis, A small amount of DNA solution is directly placed in the form of a microarray and solidified on the biochip substrate. Because the in situ synthesis method is very complicated, most of the DNA microarray chips use the synthetic spotting method. The preparation of the protein chip, due to the limitation of the method, is prepared by the method of first synthesis and then spotting. The synthetic spotting method is to accurately and quickly spot different probe samples quantitatively on positively charged nylon mem...

AI Technical Summary

Problems solved by technology

[0007] The present invention is aimed at the problems of low efficiency of biological preparation and the inability to print multiple samples at the same time, and proposes a method for preparing a microarray biochip, which uses an industrial inkjet printing nozzle to prepare a "jet-printing" microarray biochip. It can realize the rapid production of microarray biochips for multi-indicator and multi-person testing

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