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Applications of Lactobacillus plantarum in complete-strain Triticumspp silage

A technology of Lactobacillus plantarum and wheat, applied in the direction of application, microbial-based methods, animal feed raw material preservation methods, etc., can solve the problems of less research and application of wheat silage, and achieve the effect of low cost and high fermentation efficiency

Inactive Publication Date: 2012-08-22
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The raw materials of silage in my country are mainly pasture (ryegrass, alfalfa, clover) and corn, while the research and application of wheat silage are less

Method used

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  • Applications of Lactobacillus plantarum in complete-strain Triticumspp silage
  • Applications of Lactobacillus plantarum in complete-strain Triticumspp silage
  • Applications of Lactobacillus plantarum in complete-strain Triticumspp silage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Isolation, purification and identification of Lactobacillus plantarum Z3-1CGMCC No.5027

[0033] Take 10 g of silage corn stalks in Zhengzhou, Henan, add 90 mL of distilled water, shake with a shaker for 5 min, and dilute in a gradient of 10-1-10-5. Take 20 μL from each gradient dilution solution and spread it on the MRS solid medium, let it stand for anaerobic culture for 48 hours, pick a single colony with obvious differences in colony shape, size, color and gloss, and repeat the streaking until the obtained Pure colonies. The strains were inoculated in MRS liquid medium and cultured at 30°C for 24 h, centrifuged at 10,000 rpm for 10 min, and 20 μL of the supernatant was taken to inoculate Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis ), Salmonella enterica, Pseudomonas aeruginosa and Escherichia coli were respectively used as indicator bacteria, and the bacteriostasis test was carried out by the Oxford cup double plate method, as figure 1 ...

Embodiment 2

[0041] Embodiment 2, the growth of Lactobacillus plantarum (Lactobacillus plantarum) Z3-1CGMCC No.5027 under different pH, temperature and salt concentration environmental conditions

[0042] Adjust the initial pH of the MRS medium to 2.0, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 7.0, 8.0, 9.0, and 30°C with 1 mol / L hydrochloric acid and 1 mol / L sodium hydroxide, respectively Cultivate Lactobacillus plantarum (Lactobacillus plantarum) Z3-1CGMCC No.502724h at a constant temperature, without adjusting the acid during the cultivation process, and measure the light absorption value at 600nm with a spectrophotometer (OD 600 ). The results are shown in Table 2, the Z3-1 strain can grow under the conditions of initial pH2.5-pH9.0.

[0043] Inoculate the activated Lactobacillus plantarum (Lactobacillus plantarum) Z3-1CGMCC No.5027 strain into MRS liquid medium and culture them at 5, 10, 15, 20, 25, 30, 35, 40, 42, 50°C and other temperatures 24h, the light absorption value (OD) at 600nm w...

Embodiment 3

[0048] Embodiment 3, Lactobacillus plantarum (Lactobacillus plantarum) Z3-1CGMCC No.5027 silage whole plant wheat

[0049] Whole plant wheat: stems, leaves and seeds of wheat (Triticum spp.) developed to the stage of milk maturity are cut into 1-2 cm lengths, and the water content is 54%.

[0050] Method I

[0051] 1. Preparation of bacterial suspension

[0052] Under sterile conditions, Lactobacillus plantarum (Lactobacillus plantarum) Z3-1CGMCC No.5027 was inoculated in MRS liquid medium and cultured at 30°C to obtain a Z3-1 content of 10 8 ~10 9 cfu / mL bacterial suspension.

[0053] 2. Set up two treatments (each treatment is repeated 3 times, and the results are expressed in the form of average value)

[0054] Treatment 1 (inoculated with Z3-1 silage): take 100g whole plant wheat, add 10 per gram of silage raw material 5 cfu Z3-1 Take the Z3-1 bacterial suspension prepared in step 1, put it into a silage bag, mix it evenly, and store it anaerobically at 15°C to 25°C (...

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Abstract

The invention discloses applications of Lactobacillus plantarum in the complete-strain Triticumspp silage, wherein Lactobacillus plantarum is Lactobacillus plantarum Z3-1, and the accession number in China General Microbiological Culture Collection Center is CGMCC (China General Microbiological Culture Collection Center) No.5027. The bacterial strain can improve the nutritional value of stem, leaf and seed silage of Triticumspp in high milk ripe stage; escherichia coli is lower than the detection line after silage for 15 days; and butyrate which is easy to cause stink is not contained. Compared with the natural silage, inoculation Z3-1 silage is fermented for 30 days; pH is lower; the content of lactic acid in organic acid is improved by 196%; the content of acetic acid is improved by 159%; the content of crude protein is improved by 8.32%; and the content of coarse fiber is lowered by 12.6%. By applying the Lactobacillus plantarum Z3-1 CGMCC No.5027 provided by the invention, the inoculation silage complete-strain Triticumspp has the advantages of good effects and low cost and the like, and can be applied to the production of the environment-friendly biological feed.

Description

technical field [0001] The invention relates to the application of a plant lactobacillus in whole wheat silage. Background technique [0002] Studies have shown that the stems, leaves and seeds of wheat (Triticum spp.) at the milking stage can provide digestible fiber and a certain amount of energy (9.0MJ / kg, DM) for livestock, and the nutritional value is 10% to 20% higher than that of hay. Compared with corn, its crude protein content is relatively high. Whole plant silage wheat is a good feed for lactating dairy and beef cattle. Because the stems, leaves and seeds of wheat in the silage stage have high feed value, some countries and regions have begun to pay attention to the feed value of whole wheat silage. The raw materials of silage in my country are mainly grass (ryegrass, alfalfa, clover) and corn, while the research and application of wheat silage are less. Seeking suitable silage additives can improve silage fermentation, reduce silage loss, and ultimately impro...

Claims

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Application Information

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IPC IPC(8): A23K3/03C12N1/20C12R1/25
Inventor 焦浈王雁萍庞会利倪奎奎李东霞
Owner ZHENGZHOU UNIV
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