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Breeding method for bilinear ultra-small embryo hybrid rice

A hybrid rice and rice technology, which is applied in the field of breeding of two-line extra-small embryo hybrid rice, can solve the problems of low yield and the like, and achieve the effects of increasing thousand-grain weight, improving rice milling rate and increasing yield.

Inactive Publication Date: 2012-08-22
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to increase the rate of rice polishing, people start from all aspects of raw materials to processed products to improve the rate of rice polishing, but the effect is not great

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) The immature embryos of the two lines of sterile line FueS1 and restorer line 9311 were induced to form callus by in vitro tissue culture ( references : 1 Zhao Chengzhang; Zheng Kangle; Qi Xiufang. Effects of Paclobutrazol on Callus Induction, Differentiation and Strong Seedling Culture of Immature Rice Embryos. Acta Botanica (English Edition), 1990, Issue 05; 2 Chen Zhang; The relationship between the change of free amino acid pool and redifferentiation in tissue subculture. Acta Crops, 1993, 04; 3 Gaosanji. Research on high-frequency plant regeneration system of mature indica rice embryos. Master's degree thesis of Fujian Agriculture and Forestry University, 2003); With 11Gy dose of 60 Co-γ ray irradiated the callus, planted the irradiated callus and cultured M 1 R 1 Seedlings (Fu eS1 planted in an artificial climate box), mixed harvest at maturity 1 R 1 Seeds on plants (M 2 R 2 );

[0018] 2) M of Fu eS1 and restorer line 9311 2 R 2 After the seeds were d...

Embodiment 2

[0021] 1) The immature embryos of two lines of sterile line Pei'ai 64S and restorer line E32 were induced to form callus by in vitro tissue culture, and a dose of 15Gy 60 Co-γ ray irradiated the callus, planted the irradiated callus and cultured M 1 R 1 Seedlings (Fu eS1 planted in an artificial climate box), mixed harvest at maturity 1 R 1 Seeds on plants (M 2 R 2 );

[0022] 2) Pei'ai 64S and E32 M 2 R 2 After the seeds are dried, they are hulled into brown rice, and extra-small-embryo mutant brown rice grains are visually selected from the brown rice; the extra-small-embryo mutant brown rice grains of Pei'ai 64S and E32 are planted respectively (Pei'ai 64S mutant brown rice grains are planted in an artificial climate box), Using the original parents Pei'ai 64S and E32 as controls, the agronomic traits and flowering and pollination traits of all mutants were investigated at the heading and flowering stage, and the yield traits of all mutants were investigated at the m...

Embodiment 3

[0025] 1) The immature embryos of two lines of sterile line Guangzhan 63S and restorer line R2469 were taken to induce callus formation by in vitro tissue culture, and a dose of 13Gy 60 Co-γ ray irradiated the callus, planted the irradiated callus and cultured M 1 R 1 Seedlings (Fu eS1 planted in an artificial climate box), mixed harvest at maturity 1 R 1 Seeds on plants (M 2 R 2 );

[0026] 2) Widely occupy the M of 63S and R2469 2 R 2 After the seeds are dried, they are hulled into brown rice, and the extra-small-embryo mutant brown rice grains are visually selected from the brown rice; the extra-small-embryo mutant brown rice grains of Guangzhan 63S and R2469 are planted respectively (Guangzhan 63S mutant brown rice grains are planted in an artificial climate box), Using the original parents Guangzhan 63S and R2469 as controls, the agronomic traits and flowering and pollination traits of all mutants were investigated at the heading and flowering stage, and the yield ...

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Abstract

The invention discloses a breeding method for bilinear ultra-small embryo hybrid rice and belongs to the technical field of special rice seedling. Bilinear sterile line and restoring line immature embryos to be mutagenized are subjected to in vitro tissue culture induction to form callus, 60 Co-gamma rays with the dosage being 11 to 15Gy are utilized for irradiation treatment, ultra-small embryo property mutants are obtained, then, the ultra-small embryo bilinear sterile line and restoring line are breaded through selecting properties of agriculture, yield, flowering and the like, and further, the bilinear ultra-small embryo hybrid rice combination is formed through matching. The content of the embryo is reduced through the breeding method, and the endosperm specific gravity improvement is reached, so the goals of improving the grain thousand seed weight, the yield and the polished rice rate are reached. The breeding method provided by the invention has the advantages that the ultra-small embryo property mutation frequency reaches 0.01 to 0.02 percent, the embryo weight of the breaded bilinear ultra-small embryo hybrid rice only accounts for 1 to 1.2 percent of the coarse rice grain weight, the size is only 1 / 3 to 1 / 2 of that of the ordinary rice embryo, and the polished rice rate is improved by 1.5 to 2.5 percent through being compared with that of the ordinary rice.

Description

technical field [0001] The invention specifically relates to a method for breeding two-line ultra-small embryo hybrid rice, and belongs to the technical field of special rice breeding. Background technique [0002] The brown rice of rice is mainly composed of endosperm and embryo. The main component of endosperm is starch, and the embryo contains more nutrients such as protein, fat and amino acid. Therefore, some breeders increase the nutrient content of brown rice by increasing the size of the embryo, such as giant embryo rice. However, to eat the nutrients in the germ, you must eat brown rice, because the germ and seed coat have been removed (remaining in the bran) during the process of milling brown rice into polished rice, and the polished rice we eat does not contain germ. Brown rice is difficult to boil because of poor taste, and people do not like to eat it. The polished rice we eat is removed during the rice milling process, because the husk, seed coat and embryo a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 章清杞陈幼玉
Owner FUJIAN AGRI & FORESTRY UNIV
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