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Atomic force microscope probe and probe tip thereof and method for detecting capability of adhesion between helicobacter pylori and gastric mucus

A technology of atomic force microscope and Helicobacter pylori is applied in the field of atomic force microscope probe to achieve the effect of convenient research

Inactive Publication Date: 2015-01-07
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently no assays available to measure the ability of H. pylori to adhere to gastric surfaces

Method used

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  • Atomic force microscope probe and probe tip thereof and method for detecting capability of adhesion between helicobacter pylori and gastric mucus
  • Atomic force microscope probe and probe tip thereof and method for detecting capability of adhesion between helicobacter pylori and gastric mucus
  • Atomic force microscope probe and probe tip thereof and method for detecting capability of adhesion between helicobacter pylori and gastric mucus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Express the purified adhesin BabA at a concentration of 0.5 mg / mL;

[0031] (2) Synthesis of gold particles: synthesized according to the method of Langmuir, 1993, 9, 2301-2309, and the diameter of the obtained gold particles is 1-3 nanometers;

[0032] (3) Evenly cover the surface of Helicobacter pylori with a layer of gold particles (method refers to Cancer Letters, 2004, 209(2), 171-176), the thickness of the covered gold particle layer is 8-10 nanometers, and the number is 10 -5 pmol / helicobacter pylori;

[0033] (4) Adhesin BabA was modified on the surface of the gold particle layer by electrostatic adsorption (refer to J.Aam.Chem.Soc.2006, 128, 2115-2120 for the modification method), and the amount of modified adhesin was 5×10 -7 pmol / helicobacter pylori;

[0034] (5) Adhere it to the distal end of the tipless atomic force microscope probe cantilever 1 with UV-curable glue. (Refer to Front Biosci.2010, 2, 1028-41 for the adhesion method), the volume of the ...

Embodiment 2

[0048] (1) Express the purified adhesin SabA at a concentration of 1 mg / mL;

[0049] (2) Synthesis of gold particles (method refer to Langmuir, 1993, 9, 2301-2309), the diameter of the obtained gold particles is 1-3 nanometers;

[0050] (3) Evenly cover the surface of Helicobacter pylori with a layer of gold particles (method refers to Cancer Letters, 2004, 209(2), 171-176), the thickness of the covered gold particle layer is 8-10 nanometers, and the number is 10 -6 pmol / helicobacter pylori;

[0051] (4) Adhesin SabA was modified on the surface of the gold particle layer by electrostatic adsorption (refer to J.Aam.Chem.Soc.2006, 128, 2115-2120 for the modification method), and the number of modified adhesin was 10 -7 pmol / helicobacter pylori;

[0052] (5) Adhere it to the distal end of the tipless atomic force microscope probe cantilever 1 with UV-curable adhesive (refer to Front Biosci.2010, 2, 1028-41 for the adhesive method), and the thickness of the adhesive is 0.1 μm; ...

Embodiment 3

[0056] (1) Express the purified adhesin SabA at a concentration of 1 mg / mL;

[0057] (2) Synthesis of gold particles (method refer to Langmuir, 1993, 9, 2301-2309), the diameter of the obtained gold particles is 1-3 nanometers;

[0058] (3) Evenly cover the surface of Helicobacter pylori with a layer of gold particles (method refers to Cancer Letters, 2004, 209(2), 171-176), the thickness of the covered gold particle layer is 8-10 nanometers, and the number is 10 -4 pmol / helicobacter pylori;

[0059] (4) Adhesin SabA was modified on the surface of the gold particle layer by electrostatic adsorption (refer to J.Aam.Chem.Soc.2006, 128, 2115-2120 for the modification method), and the number of modified adhesin was 10 -6 pmol / helicobacter pylori;

[0060] (5) Adhere it to the distal end of the tipless atomic force microscope probe cantilever 1 with UV-curable glue. (For the adhesion method, refer to Front Biosci.2010, 2, 1028-41), the thickness of the glue is 0.5 μm;

[0061] ...

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Abstract

Provided in the invention is a tip of an atomic force microscope probe. The probe tip is characterized in that: the tip comprises: a helicobacter pylori; a gold particle layer covered on the outer surface of the helicobacter pylori; and a helicobacter pylori adhesion factor modified on the outer surface of the gold particle layer. Besides, the invention also provides an atomic force microscope probe including the tip as well as a method for detecting capability of adhesion between helicobacter pylori and gastric mucus. According to the invention, on the basis of atomic force microscopy (AFM), a condition of contact between helicobacter pylori and gastric mucus in a natural state is substantially simulated by utilizing characteristics of high precision and real-time observation of the AFM, so that it becomes possible to carry out on-site research on a submicron acting force between an adhesion factor and the gastric mucus in the scale of micron meters; and mechanical information that is obtained by utilizing a force curve is converted into adhesive work according to a formula, thereby carrying out accurate quantification on the capability of adhesion between the adhesion factor on the surface of the helicobacter pylori and the gastric mucus.

Description

technical field [0001] The invention relates to a needle tip of an atomic force microscope probe, an atomic force microscope probe and a detection method for the adhesion ability of Helicobacter pylori and gastric mucus. Background technique [0002] Current research shows that Helicobacter pylori (H. pylori) is the primary cause of gastric ulcer, gastric cancer and mucosa-associated lymphoid tissue lymphoma. According to incomplete statistics, about 50% of the world's people are infected with Helicobacter pylori. The detection of Helicobacter pylori in clinical examination and scientific research experiments is divided into two categories: invasive and non-invasive. The former refers to in situ or in vitro testing methods based on gastroscopy techniques or obtained tissue samples, mainly including: bacterial culture and staining, staining of tissue sections, rapid urokinase test and polymerase chain reaction. The latter refers to testing methods that are not based on gast...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01Q60/38
Inventor 韩东金麟陈立
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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