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Atomic force microscope probe and probe tip thereof and method for detecting capability of adhesion between helicobacter pylori and gastric mucus

An atomic force microscope, Helicobacter pylori technology, applied in the detection of Helicobacter pylori and gastric mucus adhesion ability, in the field of the needle tip of the atomic force microscope probe, to achieve the effect of convenient research

Inactive Publication Date: 2012-08-01
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently no assays available to measure the ability of H. pylori to adhere to gastric surfaces

Method used

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  • Atomic force microscope probe and probe tip thereof and method for detecting capability of adhesion between helicobacter pylori and gastric mucus
  • Atomic force microscope probe and probe tip thereof and method for detecting capability of adhesion between helicobacter pylori and gastric mucus
  • Atomic force microscope probe and probe tip thereof and method for detecting capability of adhesion between helicobacter pylori and gastric mucus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Express the purified adhesin BabA at a concentration of 0.5 mg / mL;

[0031] (2) Synthesis of gold particles: synthesized with reference to the method of Langmuir, 1993, 9, 2301-2309, the diameter of the obtained gold particles is 1-3 nanometers;

[0032] (3) Evenly cover the surface of Helicobacter pylori with one layer of gold particles (method with reference to CancerLetters, 2004, 209 (2), 171-176), the thickness of the covered gold particle layer is 8-10 nanometers, and the number is 10 -5 pmol / helicobacter pylori;

[0033] (4) Adhesin BabA was modified on the surface of the gold particle layer by electrostatic adsorption (refer to J.Aam.Chem.Soc.2006, 128, 2115-2120 for the modification method), and the amount of modified adhesin was 5×10 -7 pmol / helicobacter pylori;

[0034] (5) Adhere it to the distal end of the tipless atomic force microscope probe cantilever 1 with UV-curable glue. (The adhesion method refers to Front Biosci.2010, 2, 1028-41), the volume...

Embodiment 2

[0048] (1) Express the purified adhesin SabA at a concentration of 1 mg / mL;

[0049] (2) Synthesis of gold particles (method with reference to Langmuir, 1993, 9, 2301-2309), the diameter of the obtained gold particles is 1-3 nanometers;

[0050] (3) Evenly cover the surface of Helicobacter pylori with one layer of gold particles (method with reference to CancerLetters, 2004, 209 (2), 171-176), the thickness of the covered gold particle layer is 8-10 nanometers, and the number is 10 -6 pmol / helicobacter pylori;

[0051] (4) Adhesin SabA is modified on the surface of the gold particle layer by electrostatic adsorption (refer to J.Aam.Chem.Soc.2006, 128, 2115-2120 for the modification method), and the number of modified adhesin is 10 -7 pmol / helicobacter pylori;

[0052] (5) Adhere it to the distal end of the tip-free atomic force microscope probe cantilever 1 with UV-curable adhesive (refer to Front Biosci.2010, 2, 1028-41 for the adhesive method), and the thickness of the adh...

Embodiment 3

[0056] (1) Express the purified adhesin SabA at a concentration of 1 mg / mL;

[0057] (2) Synthesis of gold particles (method with reference to Langmuir, 1993, 9, 2301-2309), the diameter of the obtained gold particles is 1-3 nanometers;

[0058] (3) Evenly cover the surface of Helicobacter pylori with one layer of gold particles (method with reference to CancerLetters, 2004, 209 (2), 171-176), the thickness of the covered gold particle layer is 8-10 nanometers, and the number is 10 -4 pmol / helicobacter pylori;

[0059] (4) Adhesin SabA is modified on the surface of the gold particle layer by electrostatic adsorption (refer to J.Aam.Chem.Soc.2006, 128, 2115-2120 for the modification method), and the number of modified adhesin is 10 -6 pmol / helicobacter pylori;

[0060] (5) Adhere it to the distal end of the tipless atomic force microscope probe cantilever 1 with UV-curable glue. (For the adhesion method, refer to Front Biosci.2010, 2, 1028-41), the thickness of the glue is 0...

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Abstract

Provided in the invention is a tip of an atomic force microscope probe. The probe tip is characterized in that: the tip comprises: a helicobacter pylori; a gold particle layer covered on the outer surface of the helicobacter pylori; and a helicobacter pylori adhesion factor modified on the outer surface of the gold particle layer. Besides, the invention also provides an atomic force microscope probe including the tip as well as a method for detecting capability of adhesion between helicobacter pylori and gastric mucus. According to the invention, on the basis of atomic force microscopy (AFM), a condition of contact between helicobacter pylori and gastric mucus in a natural state is substantially simulated by utilizing characteristics of high precision and real-time observation of the AFM, so that it becomes possible to carry out on-site research on a submicron acting force between an adhesion factor and the gastric mucus in the scale of micron meters; and mechanical information that is obtained by utilizing a force curve is converted into adhesive work according to a formula, thereby carrying out accurate quantification on the capability of adhesion between the adhesion factor on the surface of the helicobacter pylori and the gastric mucus.

Description

technical field [0001] The invention relates to a needle tip of an atomic force microscope probe, an atomic force microscope probe and a detection method for the adhesion ability of Helicobacter pylori and gastric mucus. Background technique [0002] Current research shows that Helicobacter pylori (H. pylori) is the primary cause of gastric ulcer, gastric cancer and mucosa-associated lymphoid tissue malignant lymphoma. According to incomplete statistics, about 50% of the world's people are infected with Helicobacter pylori. The detection of Helicobacter pylori in clinical examination and scientific research experiments is divided into two categories: invasive and non-invasive. The former refers to in situ or in vitro testing methods based on gastroscopy techniques or obtained tissue samples, mainly including: bacterial culture and staining, staining of tissue sections, rapid urokinase test and polymerase chain reaction. The latter refers to testing methods that are not bas...

Claims

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Application Information

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IPC IPC(8): G01Q60/38
Inventor 韩东金麟陈立
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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