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Water culture method for banana tissue-culture seedlings

A technology for tissue culture of seedlings and bananas, applied in the agricultural field, can solve the problems of impregnation, difficulty in thorough disinfection, etc., and achieve the effects of no three-waste problem, small footprint and high production efficiency.

Inactive Publication Date: 2013-05-01
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the sand bed artificial planting stage of banana tissue culture seedlings, because sand and coconut peat are easy to carry viruses and pests, it is difficult to thoroughly disinfect, resulting in the tissue culture seedlings being easily infected by viruses, pathogenic bacteria and pests during the false planting period

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 Hydroponic banana tissue culture seedling of the present invention

[0027] Use PVC boards and water tanks as cultivation containers. Use clear water to wash off banana tissue culture seedlings (Brazilian banana, purchased from Chinese Academy of Tropical Agricultural Sciences Seedling Tissue Culture Center), the banana tissue culture seedlings cleaned up are inserted on the PVC plate, the seedling spacing is 1cm, the banana tissue culture seedlings The base of the pseudostem is higher than the liquid level of the water-based rooting medium. Water-based rooting culture solution was added to the water tank, and rooting culture was carried out at 22° C. for 15 days. The formula of the water-based rooting culture solution is: 1L of water contains: potassium nitrate 500mg, ammonium nitrate 150mg, potassium dihydrogen phosphate 110mg, magnesium sulfate 250mg, calcium chloride 330mg, EDTA disodium iron 20mg, boric acid 1.50mg, four Manganese sulfate hydrate 2.0...

Embodiment 2

[0029] Embodiment 2 Hydroponic banana tissue culture seedling of the present invention

[0030] Use PVC boards and water tanks as cultivation containers. Use clear water to wash off banana tissue culture seedlings (Brazilian banana, purchased from Chinese Academy of Tropical Agricultural Sciences Seedling Tissue Culture Center), the banana tissue culture seedlings cleaned up are inserted on the PVC plate, the seedling spacing is 1cm, the banana tissue culture seedlings The base of the pseudostem is higher than the liquid level of the water-based rooting medium. Water-based rooting culture solution was added to the water tank, and rooting culture was carried out at 35° C. for 10 days. The formula of the water-based rooting culture solution is: 1L of water contains: potassium nitrate 520mg, ammonium nitrate 100mg, potassium dihydrogen phosphate 150mg, magnesium sulfate 230mg, calcium chloride 350mg, EDTA disodium iron 40mg, boric acid 1.00mg, four Manganese sulfate hydrate 2.5...

Embodiment 3

[0032] Embodiment 3 Hydroponic banana tissue culture seedling of the present invention

[0033] Use PVC boards and water tanks as cultivation containers. Use clear water to wash off banana tissue culture seedlings (Brazilian banana, purchased from Chinese Academy of Tropical Agricultural Sciences Seedling Tissue Culture Center), the banana tissue culture seedlings cleaned up are inserted on the PVC plate, the seedling spacing is 1cm, the banana tissue culture seedlings The base of the pseudostem is higher than the liquid level of the water-based rooting medium. Water-based rooting culture solution was added to the water tank, and rooting culture was carried out at 30° C. for 12 days. The formula of the water-based rooting culture solution is: 1L of water contains: potassium nitrate 505mg, ammonium nitrate 120mg, potassium dihydrogen phosphate 136mg, magnesium sulfate 246mg, calcium chloride 333mg, EDTA disodium iron 24mg, boric acid 1.24mg, four Manganese sulfate hydrate 2.2...

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Abstract

The invention relates to a water culture method for banana tissue-culture seedlings. The method comprises the following steps of: 1) performing rooting culture on the banana tissue-culture seedlings at the temperature of between 22 and 35DEG C for 10 to 15 days in a water-based rooting culture solution; and 2) replacing the water-based rooting culture solution by using a water-based nutrient solution, performing nutrient culture at the temperature of between 20 and 35DEG C for 20 to 30 days, cupping for raising the seedlings in a greenhouse, and producing in field. The water culture method for the banana tissue-culture seedlings is easy and convenient, high in production efficiency, small in floor area, and suitable for intensive pollution-free facility cultivation or farmer scattered cultivation, and avoids a three-waste problem. By the method, the banana tissue-culture seedlings are hardly infected by viruses, pathogenic bacteria and insect pests in the temporary planting period, and can be used for scientific research, such as research on the process that blight infects bananas.

Description

technical field [0001] The invention relates to the field of agriculture, in particular, the invention relates to a method for hydroponic banana tissue culture seedlings. Background technique [0002] Banana (Musa cavendishii) belongs to the evergreen perennial large herbaceous plant of Musaceae, and is one of the main tropical fruits. At present, industrialized banana seedlings have been widely used in production in many countries. In my country, since the mid-1980s, Guangdong, Guangxi, Hainan and many other regions have successively established commercial production systems for industrialized seedlings. In recent years, more than 90% of the banana seedlings used in production are tissue culture seedlings, which can provide technical support for the industrial production of bananas. [0003] At present, the cultivation of banana tissue culture seedlings mainly includes three stages: sand bed artificial planting, greenhouse seedling cultivation, and nursery emergence. In t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00
Inventor 黄俊生刘磊梁昌聪杨腊英黄小娟
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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