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Method for measuring content of ascorbic acid in plants by utilizing ultraviolet spectroscopy

A spectrophotometry, ascorbic acid technology, applied in the field of plant physiological and biochemical analysis, can solve the problems of low accuracy, cumbersome, influence, etc., and achieve the effect of high removal efficiency and simple operation

Inactive Publication Date: 2012-07-25
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current rubophenanthroline chromogenic method is not accurate, and the operating conditions of high performance liquid chromatography are not easy to control and are more loaded down with trivial details
Moreover, the above-mentioned national standard method is determined by indirect method, which will be interfered by other substances in the sample. For example, the 2,6-dichloroindophenol titration method is interfered by other reducing substances in the sample, and the fast blue salt B chromogenic method is affected by protein.
[0003] The inventor considers that direct determination of ascorbic acid content by ultraviolet spectrophotometry is the fastest and cheapest method, but the accuracy is also easily interfered by other substances in the sample

Method used

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  • Method for measuring content of ascorbic acid in plants by utilizing ultraviolet spectroscopy
  • Method for measuring content of ascorbic acid in plants by utilizing ultraviolet spectroscopy
  • Method for measuring content of ascorbic acid in plants by utilizing ultraviolet spectroscopy

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Experimental program
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Embodiment

[0022] 1. Prepare metaphosphoric acid-acetic acid aqueous solution: mix 15g of metaphosphoric acid, 40mL of acetic acid and 250mL of water, heat and stir until the metaphosphoric acid is dissolved, and dilute to 500mL with water.

[0023] 2. Prepare ascorbic acid standard sample solution: prepare ascorbic acid solution with a concentration of 5g / L with deionized water, and dilute it with metaphosphoric acid-acetic acid solution to a concentration of 50mg / L, 40mg / L, 30mg / L, 20mg / L, and 10mg / L, 5mg / L standard solution.

[0024] 3. Obtaining the linear regression equation of the standard curve: the above-mentioned multiple standard sample solutions with different concentrations were detected by ultraviolet spectrophotometry, and the detection wavelength was 245nm, and the obtained absorbance was drawn into a standard curve, and calculated according to the Lambert-Beer law Obtain the standard curve linear regression equation (such as figure 1 shown).

[0025] 4. Preparation of ...

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Abstract

The invention discloses a method for measuring content of ascorbic acid in plants by utilizing ultraviolet spectroscopy. The method comprises the following steps of: measuring an ultraviolet absorption value of the ascorbic acid in sample extraction buffer, damaging the ascorbic acid so as to measure a background ultraviolet absorption value through an alkali treatment method, and calculating the content of the ascorbic acid through a difference. The method is characterized in that: a high-concentration alkali treatment method is adopted when a background sample is measured, so that the reduced form ascorbic acid is eliminated completely, and the influence of an alkaline environment on ultraviolet absorption of solution to be measured is avoided simultaneously. The method is simple to operate, the measurement result is accurate, and the reducing substances are not easy to get disturbed. The method is suitable for measuring sample ascorbic acid with higher numbers in botanical studies.

Description

technical field [0001] The invention belongs to the field of plant physiological and biochemical analysis, and in particular relates to a method for measuring ascorbic acid content in plants by means of ultraviolet spectrophotometry. Background technique [0002] Ascorbic acid, also known as vitamin C, is a substance necessary for maintaining life in animals and plants. As an important reducing substance in cells, ascorbic acid has important functions such as maintaining the redox potential of cells, scavenging active oxygen, and maintaining the reduced state of vitamin E. The glutathione-ascorbic acid redox system in higher animals and plants is one of the multiple electron transport chains in cells, which participates in intracellular energy metabolism, prevents the oxidation of sulfhydryl-containing proteins, and delays the process of cell aging. The research on ascorbic acid is in the ascendant, and the determination of ascorbic acid content in various biological tissue...

Claims

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Application Information

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IPC IPC(8): G01N21/33
Inventor 李英张硕侯喜林
Owner NANJING AGRICULTURAL UNIVERSITY
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