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Bio-enzyme and microorganism containing compound feed additive and preparation process thereof

A technology of compound feed and preparation process, which is applied in the direction of animal feed, animal feed, application, etc., and can solve the problems of reduced effect of additives, affecting feeding effect, and low activity.

Inactive Publication Date: 2013-11-20
LIAONING SHENNONG BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The activity of the enzyme contained in the beneficial bacteria of this invention is small, and the contained microorganisms also contain Lactobacillus acidophilus (Lactobacillus acidophilus) belonging to anaerobic bacteria. Similarly, in the production process of feed additives containing microorganisms and biological enzymes , especially the anaerobic culture process will lead to an increase in cost, and the actual feeding of Lactobacillus acidophilus will also reduce the effect of additives due to its strict living conditions, thereby affecting the effect of feeding; Lactobacillus acidophilus is a kind of live bacteria preparation strain. Because Lactobacillus acidophilus reproduces slowly, its seed cultivation time is as long as 92 to 96 hours. In the drying process of live bacteria preparation, whey powder is used as a protective agent, and the cost is relatively low. High; Bacillus subtilis is used as the enzyme preparation strain, and the fermentation liquid is spray-dried during the preparation process, which not only has high cost, but also easily reduces the number of viable Bacillus subtilis bacteria, which is not enough to meet the needs of animals for digestion and absorption of feed. The digestive effect of feed additives entering the animal body is weakened, so that it cannot effectively balance the beneficial bacteria in the animal's digestive tract and improve the digestion and utilization rate of feed

Method used

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  • Bio-enzyme and microorganism containing compound feed additive and preparation process thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0072]The liquid seed after described Bacillus subtilis and Saccharomyces cerevisiae activation is that Bacillus subtilis is inoculated in composition and is 1% peptone, 0.4% beef extract, in the seed culture medium of 0.5%NaCl, 250ml triangular bottle seed culture medium 50ml, take inclined plane The strain 1 loop was inserted into the medium, and cultured on a rotary shaker at 28-32°C, 180-200rpm for 24 hours; inoculated Saccharomyces cerevisiae into the medium composed of Saccharomyces cerevisiae, 50ml of seed medium in a 250ml triangular bottle, and took slant bacteria The first ring of seed is inserted into the culture medium, cultivated for 30 hours at 28-32°C, 180-200rpm on a rotary shaker;

[0073] The activated liquid seeds of Trichoderma reesei and Aspergillus niger were inoculated with Trichoderma reesei and Aspergillus niger in 0.5%CMC, 0.2%NaNO 3 , 0.2%K 2 HPO 4 , 0.05%KCl, 0.05%MgSO 4 ·7H 2 O, 0.0001%FeSO 4 In the culture medium, 50ml of seed medium in 250ml...

specific Embodiment approach 2

[0088] 1.1. Strain preparation

[0089] Bacillus subtilis ( Bacillus subtilis ) and Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) as live bacteria preparation strains;

[0090] 1.2. Fermentation medium preparation

[0091] Choose crushed corn and soybean meal, the ratio of corn and soybean meal is 10:1, add warm water at 40-50°C, the ratio of material to water is 1:2.5-1:3.5, mix well and heat to 75-85°C, The time is 40-50 minutes; cook at 130-135°C, 0.10-0.15MPa for 80-90 minutes, cool to 60-70°C, add high-temperature liquefaction enzyme and glucoamylase for 3-4 hours, and obtain the glucose value (DE value) above 65% and sugar content of 12-14°BX corn and soybean meal enzymatic hydrolysis saccharification solution, adjust the pH value to 4-5, then heat to 90°C for 20-30 minutes, and use it as a fermentation medium;

[0092] 1.3. Fermentation broth preparation

[0093] 1.3.1. Preparation of Bacillus subtilis fermentation broth

[0094] A. Medium

[0095] Seed...

specific Embodiment approach 3

[0142] All experimental groups were reared separately in closed piggery on concrete floor. Put the piglet feed into the feeding trough according to the wet mix (no water in the hand), and feed it 4 times a day at 8:00 in the morning, 12:00 in the afternoon, 16:00 in the afternoon and 20:00 in the evening. Provide sufficient clean drinking water with duckbill drinking fountains, keep the house temperature at 17°C to 25°C, clear the feces twice a day, keep the house clean, and use the same methods for disinfection. During the experiment, piglets were immunized according to routine immunization procedures.

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Abstract

The invention discloses a bio-enzyme and microorganism containing compound feed additive and a preparation process thereof, which solve the problems of high production cost and less viable count of beneficial microorganisms in the prior art. The bio-enzyme and microorganism containing compound feed additive is characterized in that the additive is prepared by compounding a viable organism preparation (prepared by carrying out liquid fermentation and low-temperature drying on bacillus subtilis and brewer's yeast) with an enzyme preparation (prepared by carrying out solid fermentation and drying on trichoderma reesei and Aspergillus niger); and the viable count of beneficial microorganisms in each gram of the product is greater than 8*10<9> cfu / g, the cellulase activity (refer to the quantity of cellulose degraded by each gram of the product per minute) is greater than 500 mg, the alpha-amylase activity (refer to the quantity of starch hydrolyzed by each gram of the product per minute) is greater than 3000 mg, and the protease activity (refer to the mount of an amino acid generated by caseins hydrolyzed by each gram of the product per minute) is greater than 400 mg. The bio-enzyme and microorganism containing compound feed additive disclosed by the invention has the advantages that: the feed utilization rate of livestock can be improved in a short term, the intestinal digestive flora of livestock is improved and balanced, the breeding cost is reduced, and the feed conversion rate and the animal production performance are improved; and meanwhile, the additive is simple in preparation process, low in production cost, and suitable for industrialized production.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation and feed additives, in particular to a compound feed additive containing biological enzymes and microorganisms and a preparation process thereof. Background technique [0002] With the development and improvement of microbiology and microecology, microbial feed additives are more and more widely used in the feed industry. Microbial feed additive is a preparation that utilizes active microorganisms, their metabolites, and growth-regulating substances that are beneficial to the host. It is non-toxic, non-residual, and non-polluting, and is of great significance to environmental protection and food safety. At present, the research and development of microbial feed additives at home and abroad are mainly concentrated in three categories: one is live bacterial preparations represented by Bacillus subtilis and yeast cultures, which contain active substances and live bacteria that regulate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/16A23K1/165
Inventor 宋超
Owner LIAONING SHENNONG BIOLOGICAL ENG
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