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Method for detecting type and content of organic acids in cured tobacco root secretion

A technology for root exudates and organic acids, applied in the field of agricultural production, can solve the problems such as the reports that have not yet been successfully detected, differences in secretion extraction methods and detection methods, differences in analysis methods, etc. Fast and reproducible results

Inactive Publication Date: 2012-07-18
YUNNAN AGRICULTURAL UNIVERSITY +1
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  • Abstract
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Problems solved by technology

The analysis of organic acids in root exudates by reversed-phase chromatography has been applied in crops such as rice, soybeans, and tomatoes, but there are differences in the analysis methods. The main reason is that the extraction methods and detection methods of root exudates are different due to the different types of crops.
At present, there is no successful report on the detection of low molecular weight organic acids in flue-cured tobacco root exudates

Method used

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  • Method for detecting type and content of organic acids in cured tobacco root secretion
  • Method for detecting type and content of organic acids in cured tobacco root secretion
  • Method for detecting type and content of organic acids in cured tobacco root secretion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The root exudate samples in the examples are all flue-cured tobacco root exudates.

[0023] 1. Accurately weigh 0.1000g each of oxalic acid, tartaric acid, malic acid, lactic acid, acetic acid, maleic acid, citric acid, succinic acid and fumaric acid, and dilute to 1000mL with ultrapure water to make a mass concentration of 100μg / mL organic acid mixed stock solution, the stock solution was diluted 2 times, 4 times, 8 times, 20 times, 40 times and 80 times respectively as the standard working solution.

[0024] 2. Regression was performed on the concentrations of various organic acid standard working solutions and their corresponding peak areas. See the table below for the regression equation, linear range and detection limit.

[0025] Table 1 Regression analysis and detection limits of different organic acids

[0026] Table 1 Regression analysis and detection limit of organic acid

[0027]

[0028] Note (Note): The detection limit is calculated by 3 times the sign...

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Abstract

The invention discloses a method for detecting the type and the content of organic acids in cured tobacco root secretion. The method comprises the following steps of: selecting the wavelength; selecting the chromatographic condition; preparing a standard working solution; drawing a working curve; pretreating a sample and measuring the sample; and determining the nature by using different organic acid retention time and quantifying by using a peak area external reference method to obtain the type and the content of the organic acids in the cured tobacco root secretion. By the method, the gap of detection of the type and the content of the organic acids in the cured tobacco root secretion is filled. By the method, nine organic acids can be simultaneously detected and analyzed within 10 minutes; and the method has the advantages of simplicity in operation, high detection speed, high reproducibility and high accuracy degree. The common detection equipment is used without a special instrument, and the method can be independently completed by one person.

Description

technical field [0001] The invention belongs to the technical field of agricultural production, and in particular relates to a method for detecting the type and content of organic acids in flue-cured tobacco root exudates. Background technique [0002] Organic acids are typical substances in plant root exudates, which have important modification and restriction effects on plant growth and development and root microecology. Common low-molecular-weight organic acids in root exudates include: citric acid, oxalic acid, succinic acid, tartaric acid, malic acid, acetic acid, and malonic acid. Organic acids have the functions of regulating plant cell metabolism, fixing plant nutrients, activating plant cells, and can reduce the pH value of rhizosphere soil. At the same time, they can form chelates with aluminum, iron, calcium and other metal elements to increase the solubility of phosphide. Effectively improve the utilization rate of phosphorus, thereby promoting plant growth. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 杨焕文王戈吴涛丁元明赵正雄李佛琳旦有明易建华曹红牛芸吕平刘永嘉
Owner YUNNAN AGRICULTURAL UNIVERSITY
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