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Method for preparing livestock sex control frozen sperm by adding antioxidants of CAT (Catalase) and VE (Vitamin E)

An anti-oxidant and frozen semen technology, which is applied in the field of animal reproduction, can solve problems such as damage to sex-controlled sperm, low conception rate of sex-controlled frozen sperm, and influence on the vitality and survival time of sex-controlled frozen sperm, so as to improve sperm motility and prolong sperm production. Survival time, effect of reducing peroxidative damage

Active Publication Date: 2013-06-19
内蒙古赛科星繁育生物技术(集团)股份有限公司
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Problems solved by technology

However, in actual production, compared with the frozen semen of ordinary livestock, sex-controlled frozen semen has to undergo centrifugation, dyeing, and separation processes, causing damage to sex-controlled sperm, affecting the viability and survival time of sex-controlled frozen semen after thawing, And lead to the problem of low conception rate of sexually controlled frozen sperm

Method used

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  • Method for preparing livestock sex control frozen sperm by adding antioxidants of CAT (Catalase) and VE (Vitamin E)
  • Method for preparing livestock sex control frozen sperm by adding antioxidants of CAT (Catalase) and VE (Vitamin E)
  • Method for preparing livestock sex control frozen sperm by adding antioxidants of CAT (Catalase) and VE (Vitamin E)

Examples

Experimental program
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Effect test

Embodiment

[0035] Example: Production of 2 million / tube milk cow X sex-controlled frozen semen:

[0036] 1. CAT fluid and V E Liquid preparation:

[0037] Weigh 4.2mg of CAT at room temperature, add 1ml of distilled water to dissolve, and store at -20°C in the dark, with a shelf life of one week; weigh 4mg of V E Add 1ml of DMSO to dissolve, protect from light and store at 4°C, with a shelf life of one week;

[0038] 2. Antibiotic supplementation of dairy cow essence:

[0039] Collect 6ml of fresh dairy bull semen, add 36μl linco-spectromycin, 36μl tylomycin, 48μl dongtamycin;

[0040] 3. Sperm separation:

[0041] (1) After removing the above-mentioned cow's original essence with antibiotics, use a 50 μm semen filter to remove impurities in the original essence, filter it into a new centrifuge tube, use a density meter to detect that the actual concentration of the original essence is 1.58 billion / ml, and place Store in the refrigerator at 18-20°C;

[0042] (2) Select the SX-MoFlo...

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Abstract

The invention relates to a method for preparing livestock sex control frozen sperm by adding antioxidants of CAT (Catalase) and VE (Vitamin E), belonging to the technical field of animal propagation. The method comprises the following steps: adding CAT and VE in the semen separation and frozen processes by mainly using the characteristics that the antioxidants of CAT and VE can resist ROS (Reactive Oxygen Species) carrying free radicals so as to reduce peroxidative damage to the semen caused by the ROS, effectively protect the semen physiological function in the livestock sperm separation andfrozen processes, improve the unfrozen semen viability and prolong the semen survival time, therefore, the livestock sex control frozen sperm capable of fertilizing and conceiving are prepared.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction and mainly provides E ), which effectively protects the physiological function of sperm in the process of sperm separation and freezing in livestock, improves the vitality of sperm after thawing and prolongs the survival time of sperm, so as to prepare the method of sexually controlled frozen semen of livestock capable of normal fertilization and conception. Background technique [0002] Since the industrialization of livestock sperm separation-sex control technology in 2000, it has been promoted and applied in more than ten countries around the world. However, in actual production, compared with frozen semen of ordinary livestock, sex-controlled frozen semen has to go through centrifugation, dyeing, and separation processes, causing damage to sex-controlled sperm, affecting the viability and survival time of sex-controlled frozen semen after thawing, And lead to the problem of low co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 王丽霞苏杰孙伟郭继彤周文忠胡树香丁瑞李喜和
Owner 内蒙古赛科星繁育生物技术(集团)股份有限公司
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